Histology/Immunohistology

组织学/免疫组织学

基本信息

批准号：
7728855
负责人：
DAVID V GOLD
金额：
$ 6.58万
依托单位：
CTR FOR MOLECULAR MEDICINE/IMMUNOLOGY
依托单位国家：
美国
项目类别：
财政年份：
2008
资助国家：
美国
起止时间：
2008-08-01 至 2009-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7728855
关键词：
Acetic Acid Acetic Acids Address Aftercare Aliquot Animals Antibodies Antigen-Presenting Cells Antigens Apoptosis Applications Grants Avidin B lymphoid malignancy B-Cell Neoplasm B-Lymphocytes Binding Biological Assay Biological Markers Biological Preservation Biotin Bispecific Antibodies Blood Blood Platelets Blood specimen Budgets Buffers CD19 gene CD22 antigen CD22 gene Carbon Dioxide Cell Fraction Cell Line Cell Lineage Cells Centrifugation Characteristics Class Clinical Clinical Pathology Collection Colon Carcinoma Color Computer Assisted Computer software Cooperative Human Tissue Network Core Facility Cultured Cells Cytolysis Data Dendritic Cells Densitometry Digestion Disruption Drug or chemical Tissue Distribution Emulsions Epithelial Cells Epitopes Epratuzumab Equipment Equus caballus Erythrocytes Ethanol Exhibits Ficoll Fixatives Flow Cytometry Fluorescein-5-isothiocyanate Formalin Fox Chase Cancer Center Freezing Fresh Water Frozen Sections Genus Capra Glass Glycerol Goat Gold Growth HLA-DR Antigens Hairy Cell Leukemia Haptens Heating Hematoxylin Hematoxylin and Eosin Staining Method Heterogeneity High temperature of physical object Histologic Histology Hospitals Housing Hydrogen Peroxide Image Immunoassay Immunoglobulin G Immunoperoxidase Technics Incubated Incubators Individual Institution Label Laboratories Light Light Microscope Link Liquid substance Lung Lymphocyte Lymphoid Lymphoma MS4A1 gene Malignant - descriptor Malignant Neoplasms Manufacturer Name Marrow Mature B-Lymphocyte Measures Mechanics Methanol Methods Microscopy Microtome - medical device Modification Molecular Monoclonal Antibodies Morphology Mucin-1 Staining Method Multiple Myeloma Mus Myelogenous N-hydroxysuccinimide Normal Horse Serum Normal tissue morphology Numbers Operative Surgical Procedures Osmotic Shocks Paraffin Embedding Paraffin Tissue Patients Pattern Performance Peroxidase Peroxidases Population Preparation Procedures Program Research Project Grants Program Reviews Protocols documentation Purpose Radioimmunoconjugate Radioimmunotherapy Radiolabeled Range Reagent Relative (related person)Reporting Research Personnel Retrieval Rhodamine Rhodamines Roswell Park Cancer Institute SCID Mice Serum Services Slide Sodium Chloride Solid Neoplasm Solutions Source Specimen Staining method Stains Stem cells Structure of thyroid parafollicular cell Study Section Sum Surveys Suspension substance Suspensions Techniques Technology Temperature Testing Time Tissue Banks Tissue Embedding Tissue Procurements Tissue Stains Tissues Tracer Transcriptional Activation Tube Tumor Markers Up-Regulation Water Week Work Xenograft procedure animal tissue cancer type charge coupled device camera collagenase cryogenics cryostat digital fluorescence microscope follow-up granulocyte instrument interest invariant chain kidney cell light microscopy macrophage member microautoradiography monoclonal antibody LL2 monocyte neoplastic cell paraform peripheral blood plasma cell differentiation precursor cell pressure programs radiotracer rituximab sarcoma sodium phosphate tissue fixing tositumomab tumor vector

项目摘要

CMMI currently maintains an active Histology Laboratory that has provided various histologic staining imtnunoassay procedures as a service for in-house investigators. This Core Facility will be responsible for tissue procurement, preparation, sectioning, and the performance of immunoenzyme and immunofluorescent assays, microautoradiography, and specialized histochemical procedures. Examination of stained specimens can be carried out by light microscopy, fluorescent microscopy or video densitometer. For the purposes of this grant proposal Dr. Gold, (Core Leader) will review all slides. Additional assistance in the review of stained slides is available from our collaborators in this Program Project, Drs. Ely and Knowles from NY Hospital (NYC, NY). A majority of the specimens for histology services will be provided by members of the Program Project, either from experimental animal and cell culture studies or clinical pathology material to be obtained from Drs. Ely and Knowles of the NY Hospital (NYC, NY). As a yearly average we expect 1250 specimens a year for histology services.

CMMI 目前拥有一个活跃的组织学实验室，提供各种组织学染色免疫分析程序作为内部调查员的服务。该核心设施将负责组织采购、免疫酶和免疫荧光测定、显微放射自显影的制备、切片和执行，和专门的组织化学程序。染色样本的检查可以通过光学显微镜进行，荧光显微镜或视频密度计。为了本拨款提案的目的，Gold 博士（核心领导者）将检查所有幻灯片。我们在此计划中的合作者可提供对染色载玻片审查的额外帮助项目，博士。来自纽约医院（纽约州纽约市）的 Ely 和 Knowles。大多数用于组织学服务的标本将由计划项目成员提供，来自实验动物和细胞培养研究或临床病理材料可从 Drs 获得。纽约医院的伊利和诺尔斯（纽约州纽约市）。作为年平均水平，我们预计每年提供 1250 个标本用于组织学服务。