Novel delivery of phytochemicals for sustainable crop protection

用于可持续作物保护的新型植物化学物质输送

• 批准号: BB/S018948/1
• 负责人: Toby Bruce
• 金额: $ 31.16万
• 依托单位: Keele University
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FS018948%2F1
• 关键词: Novel; delivery; phytochemicals; sustainable; crop

New solutions for managing insect pests are urgently needed due to evolution of resistance to current insecticides. Insect pests cause annual losses of US$17.7 billion to the Brazilian economy. Our project focuses on development and bioactivity testing of nanotech and metallic coordination complexes as novel delivery mechanisms for bioactive phytochemcials, to provide new options for crop protection. By using defensive non-host phytochemicals, outside the metabolome of inbred crops, we can deliver phytochemcials that crop pests have not evolved with. UK-Brazil collaboration provides an ideal opportunity to share expertise and strengthen this new and promising area of research.The Stage 1 (pump-priming) project has successfully shown that nanoformulation and complexing of phytochemicals can solve low solubility issues, improve longevity of release and alleviate phytotoxicity; three major issues that have impeded the development of botanical insecticides to date. Furthermore, our early (proof of concept) phytochemical treatments have good efficacy, causing > 80% mortality of four major insecticide resistant pest species that are plaguing Brazilian agriculture (whitefly, Bemisia tabaci; fall armyworm, Spodoptera frugiperda; Western flower thrips, Frankliniella occidentalis, and peach-potato aphid, Myzus persicae). Treatments were also shown to strongly and significantly inhibit aphid reproduction (near complete shutdown) and had highly significant repellent activity. Stage 1 established a new collaboration between Biologists at Keele University (UK) and Chemists at the Federal University of São Carlos (UFSCar, Brazil), which was further strengthened by inclusion of crop protection expertise at São Paulo State University (UNESP, Brazil). The project has benefited from Keele Nanoceutics expertise, allowing low cost nanotech technologies from medical research to be translated into the agricultural field. Investment in Stage 1 has thus provided a solid foundation for future research.The Stage 2 project will use the collaboration established in Stage 1 to move towards generating new commercially viable phytochemical treatments. Workshops and exchange visits will be held to allow research groups to share expertise and work together. We have three workshops planned: UNESP (June 2019), Keele (Jan 2020) and UFSCar (Jan 2021). Experimental work will focus on three main areas: 1) Development and optimisation of phytochemical delivery using nanoformulation and complexing techniques. Treatments will be scaled up from leaf discs to whole plants with detailed investigation of two promising routes for application: sprayable formulations and systemic uptake via root drench treatments. 2) Testing bioactivity of formulations against economically important Brazilian crop pest targets. Antibiotic, antifeedant and repellent activity will be tested for using established methods we have developed for the four species. 3) Environmental and target specificity assessment. Most of the phytochemicals and formulations we are testing are already widely used as food ingredients, in medicine or in aromatherapy. However, we will obtain evidence in order to make the case for subsequent commercialisation of treatments. Breakdown of new biodegradable formulations will be measured. Non-target effects will be assessed with mammalian cell lines, an egg parasitoid wasp Trichogramma pretiosum (a beneficial natural enemy), and the honeybee, Apis mellifera.We have shown cross-fertilisation between the disciplines involved in the project, allowing Agriculture to advance by using approaches already developed in Chemistry and Medicine. Our hypothesis is that our novel formulations could provide a means to "outsource", for crop protection, defensive phytochemicals from plants that crop pests have not evolved with and therefore are susceptible to. The Stage 2 project has considerable potential to generate much needed new tools for managing crop pests.

由于对现有杀虫剂的抗药性的进化，迫切需要新的害虫防治解决方案。害虫每年给巴西经济造成 177 亿美元的损失，我们的项目重点是纳米技术和金属配位复合物作为新型递送机制的开发和生物活性测试。对于生物活性植物化学物质，为作物保护提供新的选择通过使用防御性非宿主植物化学物质，在自交系作物的代谢组之外，我们可以为作物提供植物化学物质。英国与巴西的合作提供了一个理想的机会来分享专业知识并加强这一新的、有前途的研究领域。第一阶段（泵启动）项目已成功证明纳米制剂和植物化学物质的复合可以解决低水平的问题。溶解度问题、提高释放寿命和减轻植物毒性；迄今为止阻碍植物杀虫剂发展的三个主要问题此外，我们早期的（概念验证）植物化学处理具有良好的功效，导致>严重困扰巴西农业的四种主要抗药性害虫物种（烟粉虱、草地贪夜蛾、西花蓟马和桃蚜）的死亡率高达 80%。并显着抑制蚜虫繁殖（几乎完全停止），并具有高度显着的驱避活性，第一阶段建立了生物学家之间的新合作。基尔大学（英国）和圣卡洛斯联邦大学（UFSCar，巴西）的化学家，通过纳入圣保罗州立大学（UNESP，巴西）的作物保护专业知识进一步加强了该项目受益于基尔 Nanoceutics 的专业知识。 ，使来自医学研究的低成本纳米技术能够转化为农业领域，从而为未来的研究提供了坚实的基础。第二阶段项目将利用第一阶段建立的合作来迈向发电。我们计划举办三个研讨会：UNESP（2019 年 6 月）、Keele（2020 年 1 月）和 UFSCar（2021 年 1 月）。将重点关注三个主要领域：1）使用纳米制剂和复合技术开发和优化植物化学物质的输送，处理范围将从叶盘扩大到整个叶盘。植物，详细研究了两种有前景的应用途径：喷雾制剂和通过根部浸泡处理的全身吸收。 2) 将使用我们现有的方法测试制剂对经济上重要的巴西作物害虫目标的生物活性。 3) 我们正在测试的大多数植物化学物质和配方已广泛用作食品成分、药物或芳香疗法。为了为后续治疗的商业化提供依据，将使用哺乳动物细胞系、卵寄生蜂赤眼蜂（一种有益的天敌）和蜜蜂来评估非目标效应。我们已经展示了该项目所涉及学科之间的交叉融合，通过使用化学和医学中已经开发的方法来促进农业发展。我们的假设是，我们的新配方可以提供一种新的方法。意味着“外包”用于作物保护的防御性植物化学物质，这些植物化学物质尚未与作物害虫一起进化，因此容易受到影响。第二阶段项目具有巨大的潜力，可以产生急需的新工具来管理作物害虫。

项目成果

Editorial

社论

• DOI: 10.1017/s135577181400003x
• 发表时间: 2014
• 期刊: Organised Sound
• 影响因子: 0.6
• 作者: Blackburn M

Effects of cis-Jasmone Treatment of Brassicas on Interactions With Myzus persicae Aphids and Their Parasitoid Diaeretiella rapae.

• DOI: 10.3389/fpls.2021.711896
• 发表时间: 2021
• 期刊: Frontiers in plant science
• 影响因子: 5.6
• 作者: Ali J;Covaci AD;Roberts JM;Sobhy IS;Kirk WDJ;Bruce TJA
• 通讯作者: Bruce TJA

Valorization of Hesperidin from Citrus Residues: Evaluation of Microwave-As

柑橘渣中橙皮苷的增值：微波 As 的评价

• DOI: 10.21577/0103-5053.20220050
• 发表时间: 2022
• 期刊: Journal of the Brazilian Chemical Society
• 影响因子: 1.4
• 作者: Da Silva D