ANALYSIS AND QUANTITATION OF MUTAGENESIS

诱变的分析和定量

• 批准号: 3251373
• 负责人: THEODORE T PUCK
• 金额: $ 13.53万
• 依托单位: ELEANOR ROOSEVELT INST FOR CANCER RES
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-06-15 至 1988-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3251373
• 关键词: chromosome translocation; environment related neoplasm /cancer; gene mutation; genetic mapping; genetic markers; hybrid cells; mutagens; nucleic acid sequence

New methodologies are proposed utilizing a) hybrid Chinese hamster ovary cells containing particular human chromosomes providing human cell surface antigenic markers; b) selection methods for isolating specific mutants which have lost particular markers; c) recombinant DNA techniques for identifying specific, mapped human DNA sequences that may be lost as a result of mutation; and d) a combination of these various methodologies to detect and quantitate the frequency of translocations. These various operations will be combined in a single procedure which promises to analyze the results of exposure of these cells to physical, chemical and biological agents in terms of each of the principal mutagenic actions known to be important in human diseases: localized mutations, small deletions, large deletions, chromosome loss and translocations. In addition, this approach promises to yield quantitative measurement of the dose response of each mutagen for each type of lesion with a sensitivity many times greater than previously achieved. It is also proposed to apply these methodologies for detection of situations representing human health hazards and for study of aspects of the mutagenic process.

提出了利用 a) 杂交中国仓鼠卵巢的新方法 含有特定人类染色体的细胞提供人类细胞表面 抗原标记； b) 分离特定突变体的选择方法 失去了特定标记； c) 重组DNA技术 识别特定的、映射的人类 DNA 序列，这些序列可能会作为 突变的结果； d) 这些不同方法的组合 检测并定量易位频率。 这些各种 操作将被组合在一个单一的程序中，该程序承诺分析 这些细胞暴露于物理、化学和生物的结果 就已知的每种主要诱变作用而言， 对人类疾病很重要：局部突变、小缺失、大缺失 缺失、染色体丢失和易位。 另外，这种方法 有望对每种物质的剂量反应进行定量测量 每种类型病变的诱变剂的敏感性比 先前实现的。 还建议将这些方法应用于 检测代表人类健康危害的情况并进行研究 诱变过程的各个方面。