CELL BIOLOGY OF DEVELOPMENTAL ANOMALIES

发育异常的细胞生物学

• 批准号: 3096482
• 负责人: THEODORE T PUCK
• 金额: $ 19.21万
• 依托单位: ELEANOR ROOSEVELT INST FOR CANCER RES
• 依托单位国家: 美国
• 财政年份: 1978
• 资助国家: 美国
• 起止时间: 1978-06-01 至 1989-09-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3096482
• 关键词: cytogenetics; developmental genetics; inborn metabolism disorder; molecular pathology

This long-term research program seeks elucidation of genetic-biochemical parameters controlling differentiation and developmental processes in mammalian cells. A series of cell mutants, and hybrids is being prepared involving the standard Chinese hamster ovary culture; and cells from human and animal tissues taken at different stages of development and from various developmental disease situations. Studies will be conducted on the growth, morphological, and biochemical effects produced by cyclic AMP derivatives and selected hormones. The methodologies include somatic cell genetic analysis; study of biochemical pathways; immunogenetics; electrospin resonance and other membrane studies, scanning and transmission electron microscopy, two dimensional gel electrophoresis and molecular genetic analysis using recombinant DNA methods.

这项长期研究计划旨在阐明遗传生化 控制哺乳动物分化和发育过程的参数 细胞。 正在制备一系列细胞突变体和杂交体，涉及 标准中国仓鼠卵巢培养；以及来自人类和动物组织的细胞 取自不同发育阶段和各种发育疾病 情况。 将对生长、形态和 环AMP衍生物和选定的激素产生的生化作用。 这些方法包括体细胞遗传分析；生化研究 途径；免疫遗传学；静电纺丝共振和其他膜研究， 扫描和透射电子显微镜、二维凝胶 使用重组 DNA 方法进行电泳和分子遗传分析。

项目成果

Specificity of the cAMP-induced gene exposure reaction in CHO cells.

CHO 细胞中 cAMP 诱导的基因暴露反应的特异性。

• DOI: 10.1073/pnas.85.11.3908
• 发表时间: 1988
• 期刊: Proceedings of the National Academy of Sciences of the United States of America
• 影响因子: 11.1
• 作者: Ashall,F;Sullivan,N;Puck,TT
• 通讯作者: Puck,TT

The gene coding for a sphingolipid activator protein, SAP-1, is on human chromosome 10.

编码鞘脂激活蛋白 SAP-1 的基因位于人类 10 号染色体上。

• DOI: 10.1007/bf00293023
• 发表时间: 1985
• 期刊: Human genetics
• 影响因子: 5.3
• 作者: Inui,K;Kao,FT;Fujibayashi,S;Jones,C;Morse,HG;Law,ML;Wenger,DA
• 通讯作者: Wenger,DA

Analysis of regulation of 3-hydroxy-3-methylglutaryl coenzyme A reductase in a somatic cell mutant auxotrophic for mevalonate.

甲羟戊酸营养缺陷体细胞突变体中 3-羟基-3-甲基戊二酰辅酶 A 还原酶的调节分析。

• 发表时间: 1982
• 期刊: The Journal of biological chemistry
• 作者: Sinensky,M;Torget,R;Schnitzer-Polokoff,R;Edwards,PA
• 通讯作者: Edwards,PA

Organization of a multifunctional protein in pyrimidine biosynthesis. A domain hypersensitive to proteolysis.

嘧啶生物合成中多功能蛋白质的组织。

• DOI: 10.1042/bj2170435
• 发表时间: 1984
• 期刊: The Biochemical journal
• 作者: Rumsby,PC;Campbell,PC;Niswander,LA;Davidson,JN
• 通讯作者: Davidson,JN

Giemsa-11 technique. Applications in the chromosomal characterization of hematologic specimens.

Giemsa-11 技术。

• DOI: 10.1007/bf00274204
• 发表时间: 1982
• 期刊: Human genetics
• 影响因子: 5.3
• 作者: Morse,HG;Hays,T;Patterson,D;Robinson,A
• 通讯作者: Robinson,A