China/UK/Thailand Program on Poultry Biosafety for Salmonella, E. coli and Campylobacter (CUT-SEC)

中国/英国/泰国家禽沙门氏菌、大肠杆菌和弯曲杆菌生物安全项目 (CUT-SEC)

• 批准号: BB/R012776/1
• 负责人: Timothy Walsh
• 金额: $ 197.82万
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR012776%2F1
• 关键词: China; UK; Thailand; Program; Poultry

The world's population is increasing, particularly in low-middle income countries and by 2050 as a global community we will have to feed an additional 2 billion people. This population explosion will place greater emphasis on meat production, particularly poultry. It is estimated by the world bank that by 2030, China, India and SE Asia will supply 80% of the world meat trade which will include national consumption and international export. The question is - how will this be met? Poultry farming will need to intensify to meet these global needs with the inevitable increase in antibiotic consumption used in animal feeds despite reservations from world authorities (WHO, FAO etc.). The AMR prevalence in chicken is considerably higher than other animal sectors due to the use of uncontrolled animal feeds and the potential for cross-contamination, either during growth or at slaughter. We use antibiotics to fatten and increase poultry growth yield yet this too in causing serious antibiotic resistance in human pathogens. Antimicrobial resistance (AMR) is now deemed to be the biggest global threat facing humanity in the 21C. AMR has taken center stage as a global health issue yet most non-specialists are unaware the impact AMR will have on global populations and the potential it has of taking the treatment of infections back to the "dark ages". Hitherto, most studies around the world studying the impact of AMR have been small, one dimensional and often biased - too focused on AMR bacteria as oppose to studying the whole bacterial population. To understand the impact of AMR in the poultry sector we have established a network in Nth Thailand and mid-Eastern China (Shandong) and will undertake a comprehensive sampling strategy to examine key human food pathogens (Campylobacter, Salmonella and E. coli) in farms, the environment (water, flies, wild-birds) and community (human normal flora) and infections (urinary tract infections and gastroenteritis). Our Study (called CUT-SEC) has a comprehensive sampling platform: work package (WP) 1, chicken and duck sampling from hatcheries to slaughter; WP2, flies and wild birds; WP3, water samples and seepage; WP4, domestic animals on or near farms; WP5 Normal human rectal flora; WP6 community infections. We will analyze at least 70 samples every 3 months to examine seasonal variation and sample from Nth Thailand and Shandong. Bacteria will be analyzed by basic microbiology techniques and selected to be whole genome sequenced where we can interrogate the bacteria's whole DNA and compare it to other bacteria to see if they have spread from one sector (e.g. flies) to another (e.g. humans). This study will also sequence all the bacteria in the human gut to understand the dynamics of AMR bacterial populations. We will also undertake controlled experiments in chicken farms to monitor the spread on SEC and use mathematical models to understand how AMR pathogens spread in animals and their impact on human infections. The impact of this CUT-SEC will have immense consequences for the animal, human and economic sectors in Thailand and China. Our network is well established, has a proven track record of working together in Thailand and China and expertise to undertake this exciting and challenging proposal.

世界人口正在增加，特别是在中低收入国家，到 2050 年，作为一个全球社会，我们将不得不养活另外 20 亿人。人口爆炸将更加重视肉类生产，特别是家禽。世界银行预计，到2030年，中国、印度和东南亚将供应世界肉类贸易的80%，其中包括国内消费和国际出口。问题是——如何实现这一目标？尽管世界当局（世卫组织、粮农组织等）有所保留，但动物饲料中使用的抗生素消耗量不可避免地会增加，因此家禽养殖将需要集约化以满足这些全球需求。由于使用不受控制的动物饲料以及在生长或屠宰过程中潜在的交叉污染，鸡肉的抗菌素耐药性患病率远高于其他动物部门。我们使用抗生素来育肥并提高家禽的生长产量，但这也会导致人类病原体产生严重的抗生素耐药性。抗菌素耐药性 (AMR) 现在被认为是 21 世纪人类面临的最大全球威胁。抗菌素耐药性已成为全球健康问题的焦点，但大多数非专家并不知道抗菌素耐药性将对全球人口产生影响，也不知道它有可能使感染治疗回到“黑暗时代”。迄今为止，世界各地大多数研究 AMR 影响的研究都是小规模、一维的，而且往往存在偏见——过于关注 AMR 细菌，而不是研究整个细菌群体。为了了解抗生素耐药性对家禽业的影响，我们在泰国北部和中国东部地区（山东）建立了一个网络，并将采取全面的采样策略来检查农场中的主要人类食品病原体（弯曲杆菌、沙门氏菌和大肠杆菌） 、环境（水、苍蝇、野鸟）和社区（人类正常菌群）和感染（尿路感染和胃肠炎）。我们的研究（称为CUT-SEC）有一个全面的采样平台：工作包（WP）1，从孵化场到屠宰的鸡和鸭采样； WP2、苍蝇和野鸟； WP3、水样及渗漏； WP4，农场内或农场附近的家畜； WP5 正常人直肠菌群； WP6 社区感染。我们将每 3 个月分析至少 70 个样本，以检查季节变化，样本来自泰国北部和山东。细菌将通过基本微生物学技术进行分析，并选择进行全基因组测序，我们可以询问细菌的整个 DNA 并将其与其他细菌进行比较，看看它们是否已经从一个部门（例如苍蝇）传播到另一个部门（例如人类）。这项研究还将对人类肠道中的所有细菌进行测序，以了解 AMR 细菌种群的动态。我们还将在养鸡场进行对照实验，监测 SEC 的传播情况，并利用数学模型了解 AMR 病原体如何在动物中传播及其对人类感染的影响。此次 CUT-SEC 的影响将对泰国和中国的动物、人类和经济部门产生巨大影响。我们的网络非常完善，在泰国和中国有着良好的合作记录，并且拥有承接这一令人兴奋且具有挑战性的提案的专业知识。

项目成果

Pyrazolones Potentiate Colistin Activity against MCR-1-Producing Resistant Bacteria: Computational and Microbiological Study.

• DOI: 10.1021/acsomega.2c07165
• 发表时间: 2023-03-07
• 期刊: ACS OMEGA
• 影响因子: 4.1
• 作者: Hanpaibool, Chonnikan;Ngamwongsatit, Natharin;Ounjai, Puey;Yotphan, Sirilata;Wolschann, Peter;Mulholland, Adrian J.;Spencer, James;Rungrotmongkol, Thanyada
• 通讯作者: Rungrotmongkol, Thanyada

Strain and lineage-level methylome heterogeneity in the multi-drug resistant pathogenic Escherichia coli ST101 clone

多重耐药致病性大肠杆菌 ST101 克隆的菌株和谱系水平甲基化组异质性

• DOI: 10.1101/2020.06.07.138552
• 发表时间: 2020
• 作者: Ashcroft M

An Emerging Clone, Klebsiellapneumoniae Carbapenemase 2-Producing K. pneumoniae Sequence Type 16, Associated With High Mortality Rates in a CC258-Endemic Setting.

• DOI: 10.1093/cid/ciz1095
• 发表时间: 2020-10-23
• 期刊: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
• 作者: Andrey DO;Pereira Dantas P;Martins WBS;Marques De Carvalho F;Almeida LGP;Sands K;Portal E;Sauser J;Cayô R;Nicolas MF;Vasconcelos ATR;Medeiros EA;Walsh TR;Gales AC
• 通讯作者: Gales AC

Antibiotic resistance, stewardship, and consumption - Authors' reply.

抗生素耐药性、管理和消费——作者的回复。

• DOI: 10.1016/s2542-5196(18)30263-8
• 发表时间: 2019
• 期刊: The Lancet. Planetary health
• 作者: Collignon P

Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis.

• DOI: 10.1016/s0140-6736(21)02724-0
• 发表时间: 2022-02-12
• 期刊: Lancet (London, England)
• 作者: Antimicrobial Resistance Collaborators