China/UK/Thailand Program on Poultry Biosafety for Salmonella, E. coli and Campylobacter (CUT-SEC)

中国/英国/泰国家禽沙门氏菌、大肠杆菌和弯曲杆菌生物安全项目 (CUT-SEC)

• 批准号: BB/R012776/1
• 负责人: Timothy Walsh
• 金额: $ 197.82万
• 依托单位: CARDIFF UNIVERSITY
• 依托单位国家: 英国
• 项目类别: Research Grant
• 财政年份: 2018
• 资助国家: 英国
• 起止时间: 2018 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=BB%2FR012776%2F1
• 关键词: China; UK; Thailand; Program; Poultry

The world's population is increasing, particularly in low-middle income countries and by 2050 as a global community we will have to feed an additional 2 billion people. This population explosion will place greater emphasis on meat production, particularly poultry. It is estimated by the world bank that by 2030, China, India and SE Asia will supply 80% of the world meat trade which will include national consumption and international export. The question is - how will this be met? Poultry farming will need to intensify to meet these global needs with the inevitable increase in antibiotic consumption used in animal feeds despite reservations from world authorities (WHO, FAO etc.). The AMR prevalence in chicken is considerably higher than other animal sectors due to the use of uncontrolled animal feeds and the potential for cross-contamination, either during growth or at slaughter. We use antibiotics to fatten and increase poultry growth yield yet this too in causing serious antibiotic resistance in human pathogens. Antimicrobial resistance (AMR) is now deemed to be the biggest global threat facing humanity in the 21C. AMR has taken center stage as a global health issue yet most non-specialists are unaware the impact AMR will have on global populations and the potential it has of taking the treatment of infections back to the "dark ages". Hitherto, most studies around the world studying the impact of AMR have been small, one dimensional and often biased - too focused on AMR bacteria as oppose to studying the whole bacterial population. To understand the impact of AMR in the poultry sector we have established a network in Nth Thailand and mid-Eastern China (Shandong) and will undertake a comprehensive sampling strategy to examine key human food pathogens (Campylobacter, Salmonella and E. coli) in farms, the environment (water, flies, wild-birds) and community (human normal flora) and infections (urinary tract infections and gastroenteritis). Our Study (called CUT-SEC) has a comprehensive sampling platform: work package (WP) 1, chicken and duck sampling from hatcheries to slaughter; WP2, flies and wild birds; WP3, water samples and seepage; WP4, domestic animals on or near farms; WP5 Normal human rectal flora; WP6 community infections. We will analyze at least 70 samples every 3 months to examine seasonal variation and sample from Nth Thailand and Shandong. Bacteria will be analyzed by basic microbiology techniques and selected to be whole genome sequenced where we can interrogate the bacteria's whole DNA and compare it to other bacteria to see if they have spread from one sector (e.g. flies) to another (e.g. humans). This study will also sequence all the bacteria in the human gut to understand the dynamics of AMR bacterial populations. We will also undertake controlled experiments in chicken farms to monitor the spread on SEC and use mathematical models to understand how AMR pathogens spread in animals and their impact on human infections. The impact of this CUT-SEC will have immense consequences for the animal, human and economic sectors in Thailand and China. Our network is well established, has a proven track record of working together in Thailand and China and expertise to undertake this exciting and challenging proposal.

世界的人口正在增加，尤其是在中低水平的国家，到2050年，作为一个全球社区，我们将不得不养活20亿人。这种人口爆炸将更加重视肉类生产，尤其是家禽。世界银行估计，到2030年，中国，印度和东南亚将提供世界肉类贸易的80％，其中包括国家消费和国际出口。问题是 - 如何满足？尽管世界当局（WHO WHO，FAO等）保留了动物饲料，但由于不可避免地增加了动物饲料中使用的抗生素消费量，因此需要加强养殖以满足这些全球需求。由于使用了不受控制的动物饲料，鸡肉中的AMR患病率要比其他动物部门高得多，并且在生长期间或屠宰过程中具有交叉污染的潜力。我们使用抗生素来肥和增加家禽生长产量，但在人类病原体中引起严重的抗生素耐药性也是如此。现在，抗菌抗性（AMR）现在被视为21c人类面临的最大全球威胁。作为全球健康问题，AMR已成为中心阶段，但大多数非专家们并没有意识到AMR对全球人口的影响及其将感染治疗的潜力带回“黑暗时代”。迄今为止，世界各地研究AMR影响的大多数研究都很小，一维且经常有偏见 - 过于专注于AMR细菌，而不是研究整个细菌种群。为了了解AMR在家禽部门的影响，我们已经在泰国和中国中部（山东）建立了一个网络，并将采取全面的取样策略，以检查农场，环境，水，蝇，蝇，野生和人类（人类群体）和Infctions和Infctions（水，蝇，鸟类，鸟类，野生型）和腹部（弯曲菌，沙门氏菌和大肠杆菌）的关键饮食。我们的研究（称为CUT-SEC）具有一个全面的抽样平台：工作包（WP）1，从孵化场到屠宰的鸡肉和鸭子采样； WP2，苍蝇和野鸟； WP3，水样和渗水； WP4，农场或附近的家畜； WP5正常直肠菌群； WP6社区感染。我们将每3个月至少分析70个样本，以检查泰国和山东的季节性变化和样本。细菌将通过基本的微生物学技术进行分析，并被选为整个基因组测序，我们可以在其中询问细菌的整个DNA，并将其与其他细菌进行比较，以查看它们是否已经从一个部门（例如果蝇）与另一个部门（例如人类）进行了比较。这项研究还将对人类肠道中的所有细菌进行测序，以了解AMR细菌种群的动力学。我们还将在鸡场中进行受控的实验，以监视SEC上的扩散，并使用数学模型了解AMR病原体如何在动物中传播及其对人类感染的影响。这种裁员的影响将对泰国和中国的动物，人类和经济部门产生巨大后果。我们的网络已经建立了良好，在泰国和中国共同合作，并具有良好的往绩，并提供了这一令人兴奋且充满挑战的建议。

项目成果

Pyrazolones Potentiate Colistin Activity against MCR-1-Producing Resistant Bacteria: Computational and Microbiological Study.

• DOI: 10.1021/acsomega.2c07165
• 发表时间: 2023-03-07
• 期刊: ACS OMEGA
• 影响因子: 4.1
• 作者: Hanpaibool, Chonnikan;Ngamwongsatit, Natharin;Ounjai, Puey;Yotphan, Sirilata;Wolschann, Peter;Mulholland, Adrian J.;Spencer, James;Rungrotmongkol, Thanyada
• 通讯作者: Rungrotmongkol, Thanyada

Strain and lineage-level methylome heterogeneity in the multi-drug resistant pathogenic Escherichia coli ST101 clone

多重耐药致病性大肠杆菌 ST101 克隆的菌株和谱系水平甲基化组异质性

• DOI: 10.1101/2020.06.07.138552
• 发表时间: 2020
• 作者: Ashcroft M

An Emerging Clone, Klebsiellapneumoniae Carbapenemase 2-Producing K. pneumoniae Sequence Type 16, Associated With High Mortality Rates in a CC258-Endemic Setting.

• DOI: 10.1093/cid/ciz1095
• 发表时间: 2020-10-23
• 期刊: Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
• 作者: Andrey DO;Pereira Dantas P;Martins WBS;Marques De Carvalho F;Almeida LGP;Sands K;Portal E;Sauser J;Cayô R;Nicolas MF;Vasconcelos ATR;Medeiros EA;Walsh TR;Gales AC
• 通讯作者: Gales AC

Global burden of bacterial antimicrobial resistance in 2019: a systematic analysis.

• DOI: 10.1016/s0140-6736(21)02724-0
• 发表时间: 2022-02-12
• 期刊: Lancet (London, England)
• 作者: Antimicrobial Resistance Collaborators

Antibiotic resistance, stewardship, and consumption - Authors' reply.

抗生素耐药性、管理和消费——作者的回复。

• DOI: 10.1016/s2542-5196(18)30263-8
• 发表时间: 2019
• 期刊: The Lancet. Planetary health
• 作者: Collignon P