RENAL AMMONIA GENESIS: 15NH3 STUDIES WITH GC-MS

肾氨成因：使用 GC-MS 研究 15NH3

• 批准号: 3233007
• 负责人: ITZHAK NISSIM
• 金额: $ 14.64万
• 依托单位: CHILDREN'S HOSP OF PHILADELPHIA
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-12-01 至 1987-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3233007
• 关键词: acidity /alkalinity; alpha ketoglutarate; amides; aminoacid; ammonia; gas chromatography; glutamate dehydrogenase; glutamine; kidney metabolism; mass spectrometry; mitochondria; purine nucleotides; radiotracer; renal cortex; renal tubule

This project proposes to investigate in vitro the sources of urinary ammonia nitrogen. Of particular interest is the possibility that amino acids other than glutamine are important nitrogen donors to renal ammonia, depending upon acid-base status. The influence of pH on ammonia formation from either the amide or alpha nitrogens of glutamine is also a target of study. Emphasis is placed on the respective roles of the purine nucleotide cycle and the glutamate dehydrogenase reactions as metabolic pathways of renal ammoniagenesis. The relationship between ammoniagenesis and the metabolism of alpha-ketoglutarate, particularly the role this organic acid plays in stimulating ammonia formation from glutamate, is another investigative focus. Experiments will be performed in rat kidney cortex slices, isolated renal tubules, kidney mitochondria, and the isolated perfused kidney. A unique feature of the project is the utilization of 15N as a tracer for renal nitrogen metabolism and the utilization of gas chromatography-mass spectrometry to quantitate 15N levels in this tissue. The data so generated will be of scientific value in improving our understanding of renal nitrogen metabolism.

该项目建议在体外调查尿液来源 氨氮。 特别有趣的是氨基的可能性 谷氨酰胺以外的其他酸是对肾氨的重要氮供体， 取决于酸碱状态。 pH对氨组的影响 来自谷氨酰胺的酰胺或α硝基元也是 学习。 重点放在嘌呤核苷酸的各个作用上 循环和谷氨酸脱氢酶反应作为代谢途径 肾脏氨基发电。 氨化与 α-酮戊二酸的代谢，特别是该有机酸的作用 从谷氨酸刺激氨组中发挥作用，是另一种 调查重点。 实验将在大鼠肾脏皮层切片中进行，孤立的肾脏 小管，肾脏线粒体和孤立的灌注肾脏。 该项目的独特功能是使用15N作为示踪剂 肾氮代谢和气相色谱质量的利用 在该组织中定量15N水平的光谱法。 数据如此 生成的将具有科学价值，以提高我们对 肾氮代谢。