[16-FAPESP-BE] Lignin valorization in cellulosic ethanol plants: biocatalytic conversion via ferulic acid to high value chemicals

[16-FAPESP-BE] 纤维素乙醇工厂中的木质素增值：通过阿魏酸生物催化转化为高价值化学品

基本信息

批准号：
BB/P01738X/1
负责人：
Timothy Bugg
金额：
$ 265.34万
依托单位：
University of Warwick
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2017
资助国家：
英国
起止时间：
2017 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FP01738X%2F1
关键词：
16 FAPESP Lignin valorization cellulosic

项目摘要

Lignin is a polymer found in plant cell walls, and is the most abundant source of renewable aromatic material on Earth. Lignin therefore represents a valuable raw material for generation of renewable chemicals, which will help society to reduce its dependance on crude oil for production of chemicals and materials such as plastics. Converting lignin into renewable chemicals is a very difficult challenge, because it is very hard to break down, and it is very heterogeneous (mixture of different structural units). Researchers at Warwick University have recently discovered several soil bacteria that can break down lignin, and specific enzyme biocatalysts that can oxidise lignin, and through a BBSRC/FAPESP FAPPA award have collaborated with CTBE in Brazil to identify new lignin-degrading enzymes through genome sequencing, and to develop new "biosensors" that could be used to engineer recombinant lignin-degrading micro-organisms that could break down lignin to high-value chemicals.The proposal brings together expertise in cellulosic ethanol production and metagenomic DNA sequencing (CTBE) with expertise in biocatalytic lignin valorisation (Warwick) and biocatalysis for high value chemicals production (Manchester, UCL). The overall aim is to use synthetic biology to break down lignin to intermediate ferulic acid, which has been generated from lignin via bacterial fermentation in previous work, and then to convert ferulic acid via biocatalysis into high-value chemicals. The project will : 1) optimise a lignin stream for the project from cellulosic bioethanol production at the CTBE pilot plant; 2) convert lignin into ferulic acid from lignin using synthetic biology; 3) enzymatically convert ferulic acid into a high-value pharmaceutical chemical, L-Dopa; 4) generate high value fragrance chemicals (coniferyl acetate, isoeugenol) from ferulic acid; 5) scale up chemicals production from renewable feedstocks; 6) assess the technical and sustainability impact of the methods developed in the project.

木质素是一种存在于植物细胞壁中的聚合物，是地球上最丰富的可再生芳香材料来源。因此，木质素是生产可再生化学品的宝贵原材料，这将有助于社会减少生产化学品和塑料等材料对原油的依赖。将木质素转化为可再生化学品是一项非常艰巨的挑战，因为它很难分解，而且非常不均匀（不同结构单元的混合物）。华威大学的研究人员最近发现了几种可以分解木质素的土壤细菌，以及可以氧化木质素的特定酶生物催化剂，并通过 BBSRC/FAPESP FAPPA 奖项与巴西的 CTBE 合作，通过基因组测序识别新的木质素降解酶，并开发新的“生物传感器”，可用于设计重组木质素降解微生物，将木质素分解为高价值化学品。该提案汇集了纤维素乙醇生产和宏基因组DNA测序（CTBE）方面的专业知识以及生物催化木质素增值（沃里克）和高价值化学品生产生物催化（曼彻斯特，伦敦大学学院）方面的专业知识。总体目标是利用合成生物学将木质素分解为中间体阿魏酸，该中间体阿魏酸是在之前的工作中通过细菌发酵从木质素中产生的，然后通过生物催化将阿魏酸转化为高价值化学品。该项目将： 1) 优化 CTBE 试点工厂纤维素生物乙醇生产项目的木质素流； 2)利用合成生物学将木质素从木质素转化为阿魏酸； 3）将阿魏酸酶促转化为高价值的医药化学品L-多巴； 4）由阿魏酸生成高价值香料化学品（醋酸松柏酯、异丁子香酚）； 5) 扩大可再生原料的化学品生产规模； 6) 评估项目中开发的方法的技术和可持续性影响。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Microbial hosts for metabolic engineering of lignin bioconversion to renewable chemicals

DOI：
10.1016/j.rser.2021.111674
发表时间：
2021-10-06
期刊：
RENEWABLE & SUSTAINABLE ENERGY REVIEWS
影响因子：
15.9
作者：
Bugg, Timothy D. H.;Williamson, James J.;Alberti, Fabrizio
通讯作者：
Alberti, Fabrizio

A biocatalytic cascade for the conversion of fatty acids to fatty amines

DOI：
10.1039/c9gc02260k
发表时间：
2019-09-21
期刊：
GREEN CHEMISTRY
影响因子：
9.8
作者：
Citoler, Joan;Derrington, Sasha R.;Turner, Nicholas J.
通讯作者：
Turner, Nicholas J.

Engineered co-culture for consolidated production of phenylpropanoids directly from aromatic-rich biomass.

DOI：
10.1016/j.biortech.2023.129935
发表时间：
2023-11
期刊：
Bioresource technology
影响因子：
11.4
作者：
Micaela Chacόn;Ellen Percival;T. Bugg;N. Dixon
通讯作者：
Micaela Chacόn;Ellen Percival;T. Bugg;N. Dixon

Elucidation of microbial lignin degradation pathways using synthetic isotope-labelled lignin.

DOI：
10.1039/d2cb00173j
发表时间：
2023-01-04
期刊：
RSC CHEMICAL BIOLOGY
影响因子：
4.1
作者：
Alruwaili, Awatif;Rashid, Goran M. M.;Sodre, Victoria;Mason, James;Rehman, Zainab;Menakath, Anjali K.;Cheung, David;Brown, Steven P.;Bugg, Timothy D. H.
通讯作者：
Bugg, Timothy D. H.