CHEM. NAT. CA-P MIN. & 3-D TO COLLAGEN-CEMENTUM

化学。

• 批准号: 3223614
• 负责人: DOSUK D LEE
• 金额: $ 17.01万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-07-01 至 1995-05-07
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3223614
• 关键词: X ray crystallography; adolescence (12-20); aging; apatites; calcium phosphate; cementum; chemical structure function; collagen; computer program /software; extracellular matrix; high voltage electron microscopy; human middle age (35-64); human old age (65+); human tissue; periodontium disorder; phase change; tooth; young adult human (21-34)

The research project proposed here is designed to determine the precise chemical nature of the mineral calcium phosphate phase(s) and its three-dimensional structural relationship with the collagen fibrils in cementum. The proposed study will characterize these properties and devote considerable effort to understanding the chemical and structural changes as a function of tissue maturation and aging. Two primary extracellular components of cementum are inorganic. mineral solids and organic collagen fibrils, making up over 80 percent of the tissue mass. Chemical and structural changes of these components, which take place at the molecular level during tissue maturation, influence the quality of the tissue function (i.e., structural-mechanical support). Currently, there is very little information in the literature on the mineral composition and its relations to collagen in cementum as a function of aging. The delineation of these properties, and changes during aging and under pathological conditions, will provide critical information that will support the development of new clinical treatments and materials for periodontal diseases - an increasing health problem in the field of dentistry, particularly for the growing segment of the older population. The proposed study will make extensive use of (1) high-voltage electron microdiffraction/probe microanalysis for chemical analysis; (2) high-voltage stereomicroscopy and 3-D computer serial reconstruction for ultrastructural characterization: (3) for specimen preparation, anhydrous techniques and ultracryomicrotomy will be employed in order to circumvent mineral artifacts during preparation and analyses. These techniques represent the most powerful tools available today, and in combination should yield the most intimate knowledge of cementum minerals and collagen yet obtained.

这里提出的研究项目旨在确定精确的 矿物磷酸钙相的化学性质及其 与胶原纤维的三维结构关系 牙骨质。 拟议的研究将描述这些特性并致力于 付出相当大的努力来理解化学和结构变化 组织成熟和衰老的函数。 牙骨质的两种主要细胞外成分是无机的。矿物 固体和有机胶原纤维，占 80% 以上 组织块。 这些成分的化学和结构变化， 在组织成熟过程中发生在分子水平，影响 组织功能的质量（即结构机械支持）。 目前，文献中关于这方面的信息还很少 矿物质成分及其与牙骨质中胶原蛋白的关系 的老化。 这些特性的描述以及老化过程中的变化 在病理条件下，将提供关键信息 将支持新的临床治疗方法和材料的开发 牙周病 - 牙周病领域日益严重的健康问题 牙科，特别是针对日益增长的老年人口。 拟议的研究将广泛使用（1）高压电子 用于化学分析的微衍射/探针微量分析； (2) 高压立体显微镜和 3D 计算机串行重建 超微结构表征：（3）用于样品制备，无水 将采用技术和超低温切片术来规避 制备和分析过程中的矿物伪影。 这些技巧 代表当今最强大的工具，并将其结合起来 应该产生关于牙骨质矿物质和胶原蛋白的最深入的知识 尚未获得。