SYNAPTIC ORGANIZATION OF THE AUDITORY SYSTEM

听觉系统的突触组织

• 批准号: 3216044
• 负责人: DOUGLAS L OLIVER
• 金额: $ 18.42万
• 依托单位: UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-04-01 至 1994-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3216044
• 关键词: afferent nerve; auditory nuclei; auditory pathways; autoradiography; axon; binaural hearing; brain stem; cats; electron microscopy; gamma aminobutyrate; glycine; inferior colliculus; medial geniculate body; microscopy; neural information processing; neuroanatomy; neurochemistry; neuronal transport; neurotransmitters; sound; synapses; tritium

We will investigate the synaptic bases for processing monaural and binaural information in the inferior colliculus (IC). In order to define the anatomical substrates of neural processing in the IC, we will combine axonal transport, intracellular staining, quantitative methods, and immunocytochemistry at the light (LM) and electron microscopic (EM) levels. Each experiment will address the general hypothesis that neural responses in the IC are a product of functional zones defined by banded inputs and specific cell types. To determine whether the inputs to the IC converge to create specific functional zones, two banded inputs will be labeled in the same experiment. We postulate that bands from different sources overlap to form synaptic domains which are functionally distinct zones of neuropil in the IC. To characterize the synaptic organization of the binaural inputs from the superior olivary complex (SOC), we will use EM autoradiography and test the hypothesis that inputs from the SOC can be identified by their fine structure. We predict these inputs will provide the largest number of excitatory synapses (type 1) to the central nucleus. To identify the inhibitory inputs to the synaptic domains and determine the sources of these synapses, experiments will combine axonal transport methods to determine the inputs and immunocytochemistry at the EM level to detect GABA or glycine. Quantitative analyses will test the hypothesis that the synaptic fine structure depends on the neurotransmitter. If multiple sources of GABA or glycine-containing ending are found, the structure of each inhibitory input to the IC can be investigated. Finally, to define the functional pathways through the IC, retrograde labeling an intracellular staining methods will be used to identify specific cell types in the IC; simultaneously, an anterograde marker will be used to identify a specific ascending input to these cells. We postulate that the content of synaptic domains in relation to specific cell types will create distinct output pathways to process different aspects of auditory information.

我们将调查用于处理单膜和的突触基础 下丘（IC）中的双耳信息。 为了 定义IC中神经加工的解剖基质， 我们将结合轴突运输，细胞内染色， 定量方法和光（LM）的免疫细胞化学和免疫细胞化学 电子显微镜（EM）水平。 每个实验将解决 一般假设，即IC中的神经反应是产品 由带状输入定义的功能区域和特定单元 类型。 确定IC的输入是否收敛到创建 特定的功能区域，将在 同一实验。 我们假设来自不同来源的乐队 重叠以形成功能上不同的突触域 IC中的Neuropil区域。 表征双耳输入的突触组织 从上级橄榄综合体（SOC）中，我们将使用EM 放射自显影和检验SOC输入的假设 可以通过其精细结构来识别。 我们预测这些输入 将提供最多数量的兴奋性突触（类型1） 中央核。 识别突触域的抑制输入和 确定这些突触的来源，实验将结合 轴突运输方法确定输入和 EM水平的免疫细胞化学检测GABA或甘氨酸。 定量分析将检验突触的假设 精细的结构取决于神经递质。 如果多个 发现了GABA或含甘氨酸的结局的来源， 可以研究对IC的每个抑制输入的结构。 最后，通过IC定义功能途径， 逆行标记将使用细胞内染色方法 识别IC中的特定细胞类型；同时，一个 顺行标记将用于识别特定的上升 输入这些细胞。 我们假设突触的内容 与特定单元格类型有关的域将创建独特的 输出途径来处理听觉的不同方面 信息。