SALIVARY IMMUNE RESPONSE TO COMMENSAL ORAL BACTERIA

对口腔共生细菌的唾液免疫反应

• 批准号: 3221956
• 负责人: MICHAEL F. COLE
• 金额: $ 13.98万
• 依托单位: GEORGETOWN UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-12-01 至 1992-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3221956
• 关键词: Actinomyces; Bacteroides; Streptococcus mutans; antibacterial antibody; bactericidal immunity; enzyme linked immunosorbent assay; genetic strain; high performance liquid chromatography; host organism interaction; immunoregulation; infant human (0-1 year); laboratory mouse; longitudinal human study; microorganism culture; monoclonal antibody; oral bacteria; preschool child (1-5); saliva

The goal of this proposal is to determine the salivary immune response to selected commensal oral bacteria. The majority of diseases that afflict man are caused by exogenous pathogens. However, the principal oral diseases, dental caries and periodontal disease, are caused be endogenous bacteria that are members of the normal oral flora. Although it can be shown that the salivary immune system recognizes and responds to oral bacteria the extent to which the immune system plays a role in the regulation of the indigenous oral flora is unclear. An understanding of the mechanisms by which host protection against cariogenic and periodontopathic bacteria is mediated is essential in the development of vaccines to control these opportunistically pathogenic commensal bacteria. The bacteria chosen for study are Streptococcus mutants, Actinomyces viscosus and A. naeslundii and Bacteroides intermedius. These bacteria were selected because a) they have been implicated in coronal and root surface caries and periodontal disease and b) they represent genera that are either unique to the mouth or are oral species of general that predominate at other mucosal surfaces of the body. It is proposed to conduct a longitudinal study in infants from birth to age two years. The specific aims of this study are to 1) determine the kinetics of colonization of S. muttons, A. viscosus, A. naeslundii and B. intermedius using selective media and fluorescent antibodies, 2) determine the levels and isotypes of salivary antibodies induced in response to the colonization of the selected bacteria using an enzyme-linked immunosorbent assay (ELISA), 3) determine the specificity of antibodies induced in response to colonization by Western blot analysis 4) identify and characterize immunodominant surface antigens by use of monoclonal antibodies and high performance liquid chromatography and 5) correlate the levels, isotypes and specificity of salivary antibodies with colonization of the selected bacteria by comparing ELISA titers and Western blot profiles with the appearance and concentration of the selected bacteria in the mouth.

该提议的目的是确定唾液免疫反应 选定的共生口服细菌。 大多数受苦的疾病 人是由外源性病原体引起的。 但是，校长口头 疾病，龋齿和牙周疾病是内源性引起的 细菌是正常口腔菌群的成员。 虽然可以 表明唾液免疫系统认可并响应口服 细菌免疫系统在 土著口腔菌群的调节尚不清楚。 对 宿主保护致癌和的机制 牙周病细菌正在介导，对开发至关重要 疫苗控制这些机会性致病性共生细菌。 选择用于研究的细菌是链球菌突变体，放线菌 Viscosus和A. naeslundii和Bacteroides Intermedius。 这些细菌 之所以选择是因为a）它们与冠状和根有关 表面龋齿和牙周疾病以及b）它们代表了属 是口腔独有的，或者是一般的口腔种类 在人体的其他粘膜表面占主导地位。 提议 从出生到两岁的婴儿进行纵向研究。 这 这项研究的具体目的是1）确定动力学 S. uttons的定植，A。Viscosus，A。Naeslundii和B. Intermedius 使用选择性培养基和荧光抗体，2）确定水平 以及响应定植诱导的唾液抗体的同种型 使用酶连接的免疫吸附测定法 （ELISA），3）确定响应于 通过蛋白质印迹分析殖民化4）识别和表征 通过使用单克隆抗体和高的免疫优势表面抗原 性能液相色谱和5）将水平，同种型和 唾液抗体的特异性与选定的定殖 通过比较ELISA滴度和Western印迹轮廓与细菌 选定细菌在口中的外观和浓度。