POLYPHENOL OXIDASES AS ANTIADHESINS

多酚氧化酶作为抗粘附素

• 批准号: 2722673
• 负责人: RONALD J DOYLE
• 金额: $ 10.8万
• 依托单位: UNIVERSITY OF LOUISVILLE
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2002-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2722673
• 关键词: Actinomyces; Bacteroides gingivalis; Basidiomycetes; Streptococcus mutans; Streptococcus pyogenes; adhesin; antibacterial agents; cell line; collagen; fibronectins; hexosyltransferase; lectin; microorganism hemagglutinin; miscellaneous oxidoreductase; oral bacteria

DESCRIPTION (adapted from the applicant's abstract): Several years of research have shown that many oral diseases require bacteria. The bacteria may produce toxins or may elicit host inflammatory responses. In order for bacteria to produce oral diseases, particularly dental caries and periodontal diseases, there is a requirement that the pathogens adhere to and subsequently colonize host tissues. This research is directed to the discovery of a new class of microbial anti- adhesins. It has been observed that plant polyphenol oxidases (PPOs) can effectively abolish adhesion of oral streptococci and other pathogens to various substrata. The research will concentrate on, but not be restricted to, microbial lectin targets on oral pathogens. The proposed research will focus on S. mutans and S. sobrinus, bacteria known to be involved in human dental caries, as well as selected periodontopathogens. By use of a protease-free mushroom PPO, this research will: 1. Define how the PPO inhibits the glucan-binding lectins and glucosyltransferases of the streptococci. 2. Determine the conditions in which PPO will prevent coaggregation reactions between selected coaggregating pairs, including streptococci, actinomyces and gram-negative periodontopathogens. 3. Show that PPO reduces the ability of several oral pathogens to hemagglutinate. 4. Determine if protease activities of Porphyromonas gingivalis are reduced simultaneously with loss of adhesin function upon incubation of the cells with PPO. 5. Define the conditions describing the ability of PPO to reduce bacterial adhesion to collagen and fibronectin, extracellular matrix receptors for many oral bacteria. 6. Study how PPO abolishes the ability of Streptococcus pyogenes to bind a human laryngeal cell line and to bind to soluble fibronectin. A goal of this research is to define how PPO(s) can inactivate adhesins of oral pathogens. A long term goal is to determine if PPO can be utilized in preventing or treating oral microbial diseases.

描述（根据申请人的摘要改编）：几年的 研究表明，许多口腔疾病需要细菌。这 细菌可能产生毒素或可能引起宿主炎症反应。 为了使细菌产生口腔疾病，尤其是牙齿 龋齿和牙周疾病，有一个要求 病原体粘附并随后定居于宿主组织。这 研究针对发现新的微生物抗 - 粘合剂。已经观察到植物多酚氧化酶（PPO）可以 有效废除口服链球菌和其他病原体的粘附 到各种基质。这项研究将集中于但不是 仅限于口腔病原体上的微生物凝集素靶标。提议 研究将侧重于S. mutans和S. sobrinus，已知的细菌 参与人类龋齿，并选择 牙周病。通过使用无蛋白酶的蘑菇PPO， 研究将： 1。定义PPO如何抑制葡萄糖结合凝集素和 链球菌的葡萄糖基转移酶。 2。确定PPO阻止凝聚的条件 选定的共聚集对之间的反应，包括链球菌， 放线肌和革兰氏阴性牙周病原。 3。证明PPO降低了几种口腔病原体的能力 血凝素酸。 4.确定牙龈牙龈毒死的蛋白酶活性是否为 孵育后同时减少与粘附素功能的丧失 带有PPO的细胞。 5。定义描述PPO能力降低能力的条件 细菌粘附于胶原蛋白和纤连蛋白，细胞外基质 许多口腔细菌的受体。 6。研究PPO如何废除链球菌为链球菌结合的能力 人喉细胞系并与可溶性纤连蛋白结合。 这项研究的目标是定义PPO如何使粘附素失活 口腔病原体。一个长期目标是确定PPO是否可以 用于预防或治疗口腔微生物疾病。

项目成果

Sensitivity of bacterial coaggregation to chelating agents.

细菌共聚集对螯合剂的敏感性。

• DOI: 10.1111/j.1574-695x.2000.tb01496.x
• 发表时间: 2000
• 期刊: FEMS immunology and medical microbiology
• 作者: Taweechaisupapong,S;Doyle,RJ
• 通讯作者: Doyle,RJ

Crystallization and preliminary X-ray diffraction analysis of lectin-1 from Pseudomonas aeruginosa.

铜绿假单胞菌凝集素 1 的结晶和初步 X 射线衍射分析。

• DOI: 10.1107/s0907444903008710
• 发表时间: 2003
• 期刊: Acta crystallographica. Section D, Biological crystallography
• 作者: Karaveg,Khanita;Liu,ZhiJie;Tempel,Wolfram;Doyle,RonaldJ;Rose,JohnP;Wang,BiCheng
• 通讯作者: Wang,BiCheng

Virulence factors of Porphyromonas gingivalis are modified by polyphenol oxidase and asparaginase.

牙龈卟啉单胞菌的毒力因子被多酚氧化酶和天冬酰胺酶修饰。

• DOI: 10.1034/j.1399-302x.2003.00092.x
• 发表时间: 2003
• 期刊: Oral microbiology and immunology
• 作者: Budu,CE;Luengpailin,J;Reyes,G;Doyle,RJ;Cowan,MM
• 通讯作者: Cowan,MM