THE ENZYMOLOGY OF DIOXYGEN

双氧的酶学

基本信息

批准号：
3224522
负责人：
HOWARD S. MASON
金额：
$ 8.89万
依托单位：
OREGON HEALTH & SCIENCE UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1975
资助国家：
美国
起止时间：
1975-06-01 至 1988-05-31
项目状态：
已结题

项目摘要

We are seeking principles which govern biological activation of molecular oxygen. Our objective is a generalized understanding of mechanisms by which oxygen supports life in terms of structures of oxygenases, oxidases, oxygen, co-factors, and substrates. We propose to investigate selected dioxygenase, mixed function oxidases, and oxidase reactions in which copper, iron, (non-heme) and heme are prosthetic groups. We will continue our biochemical and biophysical studies by (1) determining the structures of active sites and related protein structures of copper, heme, and iron oxygenase and mixed function oxidases, (2) characterizing reactions which take place between these oxygenases, their substrates and O2, in terms of oxygen- and substrate-containing intermediates, (3) detecting and characterizing intermediates by specific transfer of (isotopic) oxygen to substrates, (5) establishing by kinetic and thermodynamic studies the sequence of formation and reaction of intermediates, and (6) separating the native subunits of beef heart cytochrome c oxidase, characterizing them chemically and physically, and reconstituting the functional aggregate oxidase with them both to establish the native character of the isolated subunits, and to determine the nature of the associations which occur when the functional enzyme is reconstituted from the subunits.

我们正在寻求控制分子生物激活的原则氧。我们的目标是对机制的广泛理解氧在氧化酶，氧化酶，氧气，氧气的结构方面支持寿命联合因素和底物。我们建议研究选定的二氧酶，混合功能氧化酶和氧化酶反应，其中铜，铁，（非血红素）血红素是假肢。我们将通过（1）确定活性位点的结构和铜，血红素和相关蛋白质结构的结构铁氧酶和混合功能氧化酶，（2）表征反应以这些加氧酶，其底物和O2发生在（3）检测和表征含氧和底物的中间体通过（同位素）氧对底物的特异性转移中间体，（5）通过动力学和热力学研究建立形成和中间体的反应，（6）分离牛肉心的天然亚基细胞色素C氧化酶，化学和物理表征它们，以及与它们重新建立功能聚集氧化酶以建立孤立亚基的本地特征，并确定当功能酶从从该功能酶重组时发生的关联亚基。