ANESTHETIC ACTIONS ON INHIBITORY GABAERGIC INTERNEURONS

对抑制性伽巴能中间神经元的麻醉作用

• 批准号: 6019331
• 负责人: M. Bruce MacIver
• 金额: $ 19.74万
• 依托单位: STANFORD UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1997
• 资助国家: 美国
• 起止时间: 1997-08-01 至 2001-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6019331
• 关键词: GABA receptor; action potentials; general anesthesia; interneurons; pharmacokinetics; voltage /patch clamp

DESCRIPTION: (Adapted from the applicant's abstract) The goal of the investigators is to understand mechanisms of general anesthetic action. Attenuated neuronal excitability during anesthesia results in part from enhanced GABA-mediated synaptic inhibition. The major direction of this proposal is to investigate anesthetic actions on inhibitory GABAergic interneurons. Interneurons mediate synaptic inhibition by releasing GABA. Whole-cell recordings and infrared imaging make it now possible to study anesthetic actions on previous difficult to access interneurons. Unexpectedly, because general anesthetics usually depress transmitter release, is the preliminary finding that general anesthetics increase the spontaneous release of GABA from inhibitory interneurons. Enhanced GABA release can combine with anesthetic-postsynaptic GABA receptor actions to augment inhibition. The proposed study will identify the effects of anesthetics on inhibitory GABAergic interneurons, characterize the mechanisms underlying these actions, and determine the consequences of these effects on neuronal excitability. The investigators believe that these studies on inhibitory GABAergic interneurons will provide information regarding mechanisms of anesthetic action.

描述：（改编自申请人的摘要）该项目的目标 研究人员的目的是了解全身麻醉作用的机制。 麻醉期间神经元兴奋性减弱的部分原因是 增强 GABA 介导的突触抑制。 这个的主要方向 提案是研究对抑制性 GABA 的麻醉作用 中间神经元。 中间神经元通过释放 GABA 介导突触抑制。 全细胞记录和红外成像使研究成为可能 对先前难以接近的中间神经元进行麻醉作用。 出乎意料的是，因为全身麻醉药通常会抑制神经递质 释放，是初步发现，全身麻醉会增加 抑制性中间神经元自发释放 GABA。 增强型GABA 释放可以与麻醉突触后 GABA 受体作用结合 增强抑制作用。 拟议的研究将确定以下因素的影响： 麻醉剂对抑制性 GABA 能中间神经元的作用，表征 这些行为背后的机制，并确定这些行为的后果 对神经元兴奋性的影响。 调查人员认为，这些 抑制性 GABA 能中间神经元的研究将提供信息 关于麻醉作用机制。

项目成果

Agent-selective effects of volatile anesthetics on GABAA receptor-mediated synaptic inhibition in hippocampal interneurons.

挥发性麻醉剂对海马中间神经元 GABAA 受体介导的突触抑制的药物选择性影响。

• 发表时间: 2001-02
• 影响因子: 8.8
• 作者: Nishikawa, K;MacIver, M B
• 通讯作者: MacIver, M B

Multiple synaptic and membrane sites of anesthetic action in the CA1 region of rat hippocampal slices.

大鼠海马切片 CA1 区多个突触和膜麻醉作用位点。

• 发表时间: 2004-12-03
• 影响因子: 2.4
• 作者: Pittson, Sky;Himmel, Allison M;MacIver, M Bruce
• 通讯作者: MacIver, M Bruce

Membrane and synaptic actions of halothane on rat hippocampal pyramidal neurons and inhibitory interneurons.

氟烷对大鼠海马锥体神经元和抑制​​性中间神经元的膜和突触作用。

• 发表时间: 2000-08-15
• 作者: Nishikawa, K;MacIver, M B
• 通讯作者: MacIver, M B