LIPID NUTRITION, MEMBRANE FLUIDITY, AND ENZYMES

脂质营养、膜流动性和酶

• 批准号: 3219693
• 负责人: SYED Q ALAM
• 金额: $ 5.7万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 1987-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219693
• 关键词: adenosinetriphosphatase; adenylate cyclase; cell membrane; cholesterol; dietary lipid; enzyme induction /repression; essential fatty acids; ion transport; linoleate; membrane lipids; nutrition related tag; ouabain; salivary glands; submandibular gland; unsaturated fats

The nature of dietary lipids and their levels can modify the structure and function of salivary glands in several different ways. A likely mode of action would be by virtue of their effect in inducing physiochemical modifications of plasma membrane lipids. Since membrane lipids play an important role in determining the activities of several membrane associated enzymes such as (Na+ + K+)-ATPase and adenyl cyclase in a number of tissues, it is quite likely that the activities of these two enzymes will be affected in salivary glands as well. If so, this may have a profound influence on salivary gland function because of the physiological importance of (Na+ + K+)-ATPase in active transport of ions and that of adenyl cyclase in the secretion of salivary proteins. The objective of the proposed studies is to test the above hypothesis using several nutritional models such as essential fatty acid deficiency, feeding of saturated versus unsaturated fats, cholesterol supplementation and trans fatty acids. Male weanling rats will be fed purified diets containing different levels of linoleic acid, trans fatty acids, cholesterol and two types of fats. At various time intervals, rats will be killed, their submandibular salivary glands (SMSG) will be dissected out and plasma membranes will be prepared. The activities of ouabain sensitive (Na+ +K+)-ATPase (EC 3.6.1.3) and adenyl cyclase (EC 4.6.1.1) will be determined in plasma membrane preparations and in gland homogenates. Fluidity of membranes will be determined by fluorescence spectroscopy using fluorescent probes. Aliquots of SMSG homogenates and plasma membranes will be extracted for lipids and the major phospholipids will be isolated by thin layer chromatography. The fatty acid composition of total lipids and phospholipids will be determined by gas chromatographic methods using packed and open tubular glass capillary columns. All data will be statistically analyzed in order to seek any correlations between the lipid composition, membrane fluidity and the activities of the two enzymes. Samples of submadibular saliva will also be obtained from some rats by duct cannulation. Salvia will be analyzed for total proteins, amylase, Na+ and K+. The effect of lipid nutrition on salivary gland plasma membrane lipid composition, fluidity, enzyme activities and saliva composition will be assessed.

饮食脂质及其水平的性质可以改变结构和 唾液腺的功能以几种不同的方式。 可能的模式 动作将是由于它们在诱导生理化学上的作用 质膜脂质的修饰。 由于膜脂质发挥 在确定几个膜相关的活动中的重要作用 酶，例如（Na++ K+） - ATPase和腺基环化酶在许多中 组织，这两种酶的活性很可能 也受到唾液腺的影响。 如果是这样，这可能会有深刻的 由于生理的影响 （Na++ K+）的重要性 - 离子主动运输中的ATPase和 唾液蛋白分泌中的腺基环化酶。 目的 拟议的研究是使用几种营养来检验上述假设 诸如必需脂肪酸缺乏，饱和与饱和度的模型 不饱和脂肪，补充胆固醇和反式脂肪酸。 男性 断奶大鼠将被喂食纯净的饮食，其中含有不同水平的饮食 亚油酸，反式脂肪酸，胆固醇和两种类型的脂肪。 在 各种时间间隔，将杀死大鼠，下颌下唾液 将剖析腺体（SMSG），并准备质膜。 Ouabain敏感（Na++K+）的活动 - ATPase（EC 3.6.1.3）和 将在质膜中确定腺基环化酶（EC 4.6.1.1） 制剂和腺体匀浆。 膜的流动性将是 使用荧光探针通过荧光光谱法确定。 等分试样 SMSG匀浆和质膜的含量将用于脂质和 主要的磷脂将通过薄层色谱分离。 这 将确定总脂质和磷脂的脂肪酸组成 通过使用包装和开放管状玻璃的气相色谱法 毛细管柱。 所有数据将进行统计分析，以便 寻求脂质成分，膜流动性和 两种酶的活动。 子菊花唾液的样本将 也可以通过管道插管从一些大鼠那里获得。 萨尔维亚会 分析了总蛋白，淀粉酶，Na+和K+。 脂质的效果 唾液腺质膜脂质组成，流动性，流动性，营养 将评估酶活性和唾液成分。