LIPID NUTRITION, MEMBRANE FLUIDITY AND SALIVARY ENZYMES

脂质营养、膜流动性和唾液酶

• 批准号: 3219696
• 负责人: SYED Q ALAM
• 金额: $ 14.63万
• 依托单位: LSU HEALTH SCIENCES CENTER
• 依托单位国家: 美国
• 财政年份: 1983
• 资助国家: 美国
• 起止时间: 1983-09-01 至 1994-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3219696
• 关键词: G protein; adenosinetriphosphatase; adenylate cyclase; adrenergic receptor; biological fluid transport; cell membrane; cholesterol; dietary lipid; enzyme activity; enzyme induction /repression; essential fatty acids; fluoridation; forskolin; guanine nucleotide binding protein; ion transport; laboratory rat; linoleate; membrane lipids; membrane proteins; nutrition related tag; ouabain; potassium channel; salivary glands; sodium channel; submandibular gland; unsaturated fats

We have been studying the basic biochemical mechanisms whereby the type of dietary lipids can affect the salivary gland cell function. The working hypothesis being investigated is that dietary lipids can induce changes in structural lipids and fluidity of the cell membranes, which results in modification of membrane function. In vivo changes can be induced in membrane lipids by essential fatty acid deficiency, cholesterol-rich diets and diets containing high levels of omega-3 fatty acids. Changes in membrane lipids result in altering beta-receptor adenylate cyclase system, which consists of the receptor, G-proteins and adenylate cyclase. Further studies will be conducted using these nutritional models to determine their effects on each of the 3 components of the adenylate cyclase system in the rat submandibular salivary gland (SMSG). Studies will also be conducted with dispersed SMSG cells from rats fed various diets in order to determine if the diet-induced changes in membrane lipids and adenylate cyclase system would result in. functional changes such as the secretion of glycoproteins. Diet-induced changes in membrane fluidity will be assessed by measuring fluorescence polarization and lifetimes of the excited states for diphenylhexatriene and 16-anthroyloxy palmitate after their incorporation into the SMSG membranes. Because of the microsomal fatty acid desaturases are considered to be important in the regulation of membrane fluidity and in the metabolism of essential fatty acids, their specific activities in the SMSG, along with their dietary modulation, will be studied.

我们一直在研究基本的生化机制 饮食脂质的大量会影响唾液腺细胞功能。 工作 研究假设是饮食脂质可以引起变化 细胞膜的结构脂质和流动性，这导致 修改膜功能。 可以诱导体内变化 膜脂质通过必需脂肪酸缺乏，富含胆固醇的饮食 和含有高水平的omega-3脂肪酸的饮食。 变更 膜脂质导致β受体腺苷酸环化酶系统改变， 由受体，G蛋白和腺苷酸环化酶组成。 更远 研究将使用这些营养模型来确定其 对腺苷酸环化酶系统的三个组件中的每个组成部分的影响 大鼠下颌唾液腺（SMSG）。 还将进行研究 从大鼠分散的SMSG细胞中，喂养各种饮食以确定 如果饮食引起的膜脂质和腺苷酸环化酶系统的变化 会导致。功能变化，例如分泌糖蛋白。 饮食引起的膜流动性变化将通过测量来评估 激发态的荧光极化和寿命 掺杂后，二苯基甲酰胺和16片棕榈酸酯 进入SMSG膜。 由于微粒体脂肪酸去饱和酶 被认为在调节膜流动性和 在必需脂肪酸的代谢中，它们在 将研究SMSG及其饮食调节。