HUMAN PAPILLOMAVIRUSES IN GENITAL TRACT DYSPLASIAS

生殖道发育不良中的人乳头瘤病毒

• 批准号: 3182987
• 负责人: FELIX O WETTSTEIN
• 金额: $ 12.21万
• 依托单位: UNIVERSITY OF CALIFORNIA LOS ANGELES
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-04-01 至 1990-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3182987
• 关键词: DNA methylation; Papillomavirus; aneuploidy; antiviral antibody; cervix neoplasms; genetic manipulation; genetic mapping; histology; human papillomavirus; human tissue; laboratory rabbit; lysogeny; nucleic acid hybridization; oncogenic virus; plasmids; preneoplastic state; serology /serodiagnosis; synthetic antigens; viral carcinogenesis; virus DNA; virus RNA; virus antigen; virus protein; virus related neoplasm /cancer; warts

The long term goals of this research are to provide evidence for and an understanding of the role of human papilloma viruses in the development of cervical dysplasias and invasive carcinomas. The specific aims include: 1) Establish a correlation between HPV types DNA ploidy patterns and morphometric parameters seen in histologic sections. 2) a) Characterize the state of viral DNA. b) Map viral transcripts. c) Identify "early" viral proteins and elucidate their function in cervical carcinomas and carcinoma derived cell lines. 3) Investigate aspects of the immune response in papillomavirus infections. a) Determine the presence of antibodies to "early" viral proteins. b) Attempt to construct type specific antigens for "oncogenic" HPV"s and search for antibodies to these antigens in human sera which may provide an indication about the spread of these viruses in the human population.

这项研究的长期目标是提供证据和 了解人类乳头状瘤病毒在发展中的作用 宫颈发育不良和侵入性癌。 具体目的包括： 1）建立HPV类型的DNA酶模式与 在组织学部分中看到的形态计量参数。 2）a）表征病毒DNA的状态。 b）地图病毒转录本。 c）识别“早期”病毒蛋白并阐明其在宫颈中的功能 癌和癌衍生的细胞系。 3）研究乳头瘤病毒感染中免疫反应的各个方面。 a）确定对“早期”病毒蛋白的抗体的存在。 b）试图构建“致癌” hpv” s的特定抗原和 在人类血清中寻找对这些抗原的抗体，这可能会提供 这些病毒在人群中的传播的指示。

项目成果

DNA ploidy analysis of cervical condyloma and intraepithelial neoplasia in specimens obtained by punch biopsy.

穿刺活检标本中宫颈湿疣和上皮内瘤变的 DNA 倍体分析。

• 发表时间: 1989
• 期刊: Analytical and quantitative cytology and histology
• 作者: Fu,YS;Cheng,L;Huang,I;Huang,S;Wiesmeier,E;Wettstein,F;Weissman,M
• 通讯作者: Weissman,M

Correlative study of human papillomavirus DNA, histopathology and morphometry in cervical condyloma and intraepithelial neoplasia.

宫颈尖锐湿疣和上皮内瘤变中人乳头瘤病毒DNA、组织病理学和形态学的相关性研究。

• DOI: 10.1097/00004347-198812000-00001
• 发表时间: 1988
• 期刊: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists
• 作者: Fu,YS;Huang,I;Beaudenon,S;Ionesco,M;Barrasso,R;deBrux,J;Orth,G
• 通讯作者: Orth,G

Immunoreactivity of involucrin in cervical condyloma and intraepithelial neoplasia.

宫颈尖锐湿疣和上皮内瘤变中外皮蛋白的免疫反应性。

• 发表时间: 1986
• 期刊: International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists
• 作者: Saito,K;Saito,A;Fu,YS;Cheng,L;Hilborne,LH
• 通讯作者: Hilborne,LH