ACTIVATION OF PRIMORDIAL GERM CELLS TO FORM TERATOMAS

激活原始生殖细胞形成畸胎瘤

• 批准号: 3186732
• 负责人: IVAN DAMJANOV
• 金额: $ 13.87万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-07-01 至 1987-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3186732
• 关键词: cell differentiation; cell transformation; embryo /fetus; germ cells; gestational age; growth factor; heterozygote; histochemistry /cytochemistry; immunochemistry; mature animal; membrane activity; neoplastic transformation; reproductive tissue transplantation; teratoma; tissue /cell culture

This project is the continuation of the studies aimed at defining and further characterizing the factors that control the activation, proliferation, and differentiation of normal primordial germ cells in the mouse and their transition into the stem cells of benign and malignant teratomas. In the previous granting period, we have established and defined the optimal conditions for isolation and in vitro culturing of germ cells and the production of tumors. We shall now continue studying the tumorigenesis in this system by using a new recombinant inbred strain with a high spontaneous tendency for germ cell activation in transplanted genital ridges and will define the role of the host-related factors in the control of tumor formation. Special attention will be paid to hormonal and immune factors and the control of malignancy of tumors. Using the monoclonal antibody techniques, we shall define the stages of ontogenesis of primordial germ cells in the mouse and define the changes that hallmark their transition into tumor cells or differentiated testicular gonocytes. The pregonadal stages of germ cell ontogenesis will be studied to define their phenotype characteristics, developmental, and tumorigenic potential. The ultimate goal of these studies is to better our understanding of germ cell tumorigenesis in the gonad and the normal ontogenesis of germ cells that preceeds tumor formation. Specifically, in the next year we will: (1)\continue studies on the production of teratocarcinomas from genital ridges transplanted to the adult host; (2)\concentrate on the activation of germ cells with lectins; (3)\determine the nature of lectin binding to germ cells and their derivatives; (4)\continue to produce and characterize monoclonal antibodies reactive with mouse germ cells and their malignant derivatives; and (5)\continue to study the human germ cell tumors with antibodies produced to cell surface specific markers. (M)

该项目是旨在定义和 进一步表征控制激活的因素， 正常原始生殖细胞的增殖和分化 小鼠及其向良性和恶性干细胞的转变 畸胎瘤。 在上一个授予期间，我们已经建立并 确定了细菌分离和体外培养的最佳条件 细胞和肿瘤的产生。 我们现在要继续研究 通过使用新的重组近交株在该系统中进行肿瘤发生 移植后生殖细胞活化的高度自发倾向 生殖脊并将定义宿主相关因素在 控制肿瘤的形成。 将特别关注荷尔蒙和 免疫因素与肿瘤恶性肿瘤的控制。 使用 单克隆抗体技术，我们将定义个体发生的阶段 小鼠原始生殖细胞的变化并定义标志性的变化 它们转变为肿瘤细胞或分化的睾丸生殖细胞。 将研究生殖细胞个体发生的性腺前阶段以确定 它们的表型特征、发育和致瘤潜力。 这些研究的最终目标是更好地了解细菌 性腺中的细胞肿瘤发生和生殖细胞的正常个体发生 在肿瘤形成之前。 具体来说，明年我们将： (1)\继续研究生殖器产生畸胎癌 移植到成年宿主的脊； (2)\集中精力激活 具有凝集素的生殖细胞； (3)\测定凝集素与细菌结合的性质 细胞及其衍生物； (4)\继续生产和表征 与小鼠生殖细胞及其恶性细胞发生反应的单克隆抗体 衍生品； (5)\继续研究人类生殖细胞肿瘤 产生针对细胞表面特异性标记的抗体。 (男)