EMBRYO-DERIVED TERATOCARCINOMA

胚胎衍生畸胎瘤

• 批准号: 3166034
• 负责人: IVAN DAMJANOV
• 金额: $ 13.95万
• 依托单位: THOMAS JEFFERSON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-12-01 至 1988-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3166034
• 关键词: adenosinetriphosphatase; alkaline phosphatase; angiogenesis; autoradiography; cell growth regulation; cell transformation; clone cells; developmental genetics; electron microscopy; embryo /fetus; embryo /fetus tissue /cell culture; freeze etching; gene expression; hematopoietic stem cells; histochemistry /cytochemistry; host neoplasm interaction; immunochemistry; lactation; mammalian embryology; neoplasm /cancer genetics; neoplasm /cancer transplantation; neoplastic growth; placental transfer; pregnancy; pregnancy immunology; scanning electron microscopy; teratoma; thymectomy; tumor antigens

The overall objective of this research is to study the epigenetic factors that favor, modify, or inhibit the expression of the malignant phenotype of undifferentiated embryonic cells and stem cells of teratocarcinoma. To this end teratocarcinomas are produced from 7-day mouse embryos transplanted under the kidney of immunologically compatible recipients. It has been shown that the yield of malignant tumors depends on host-related and embryo-related factors that either promote or inhibit teratocarcinogenesis. Among the host-related factors, the immune factors play a significant role. The genome of the embryo and the maternally transmitted factors also influence the teratocarcinogenesis. The malignant stem cells of murine teratocarcinomas, like their putative precursors in the embryo, express on their surface a fucopentaose moiety that can be recognized with monoclonal antibodies. Upon differentiation of either embryonic or tumor cells, this surface marker disappears from the cell surface. Antibodies recognizing this stage-specific embryonic antigen react with many tissues in the adult mouse and even humans and are thus not restricted or uniquely specific for mouse embryonal carcinoma cells. Human embryonal carcinoma cells, on the other hand, display surface characteristics that make them distinct from the equivalent mouse tumor cells. (M)

本研究的总体目标是研究表观遗传因素 有利于、改变或抑制恶性表型的表达 未分化胚胎细胞和畸胎癌干细胞。 到 最终，畸胎瘤是由 7 天的小鼠胚胎产生的 移植到免疫相容的受体的肾下。 它 研究表明，恶性肿瘤的发生率取决于宿主相关 以及促进或抑制的胚胎相关因素 畸胎癌发生。 在宿主相关因素中，免疫因素 发挥重要作用。 胚胎和母体的基因组 传播因素也影响畸胎癌的发生。 恶性的 鼠畸胎癌的干细胞，就像它们假定的前体一样 胚胎，在其表面表达岩藻五糖部分，该部分可以 用单克隆抗体识别。 区分任一 胚胎或肿瘤细胞，该表面标记从细胞中消失 表面。 识别这种阶段特异性胚胎抗原的抗体 与成年小鼠甚至人类的许多组织发生反应，因此不会 对小鼠胚胎癌细胞具有限制性或独特特异性。 人类 另一方面，胚胎癌细胞显示表面 使它们与同等小鼠肿瘤不同的特征 细胞。 (男)

项目成果

Rat serum promotes the in vitro development of mouse blastocysts during early somitic stages of embryogenesis.

大鼠血清促进胚胎发生早期体细胞阶段小鼠囊胚的体外发育。

• DOI: 10.1002/jez.1402170318
• 发表时间: 1981
• 期刊: The Journal of experimental zoology
• 作者: Wu,TC;Wan,YJ;Damjanov,I
• 通讯作者: Damjanov,I

Development of early somitic mouse embryos in static culture in vitro.

体外静态培养中早期体细胞小鼠胚胎的发育。

• DOI: 10.1002/jez.1402200210
• 发表时间: 1982
• 期刊: The Journal of experimental zoology
• 作者: Wan,YJ;Wu,TC;Damjanov,I
• 通讯作者: Damjanov,I

Antibody-affinity purification of novel alpha-L-fucosidase from mouse liver.

从小鼠肝脏中抗体亲和纯化新型 α-L-岩藻糖苷酶。

• DOI: 10.1042/bj2450589
• 发表时间: 1987
• 期刊: The Biochemical journal
• 作者: Laury-Kleintop,LD;Damjanov,I;Alhadeff,JA
• 通讯作者: Alhadeff,JA

Immunohistochemical localization of the mouse stage-specific embryonic antigen 1 in human tissues and tumors.

• 发表时间: 1983-02
• 期刊: Cancer research
• 影响因子: 11.2
• 作者: N. Fox;I. Damjanov;B. Knowles;D. Solter

Lectin histochemistry of classic and spermatocytic seminoma.

经典精原细胞瘤和精原细胞瘤的凝集素组织化学。

• 发表时间: 1985
• 期刊: Archives of pathology & laboratory medicine
• 影响因子: 4.6
• 作者: Lee,MC;Talerman,A;Oosterhuis,JW;Damjanov,I
• 通讯作者: Damjanov,I