B-LACTAMASE AND DEVELOPMENT OF MYXOCOCCUS XANTHUS

B-内酰胺酶和黄色粘球菌的发育

• 批准号: 6018424
• 负责人: JOHN R KIRBY
• 金额: $ 3.17万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-07-01 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/6018424
• 关键词: Myxococcus; bacterial genetics; beta lactamase; cell wall; gene expression; gene mutation; microorganism growth; polymerase chain reaction

The goal of this research project is to describe the role of the inducible beta-lactamase system in the developmental program of Myxococcus xanthus. Recent evidence indicates that M.xanthus will undergo starvation- independent sporulation with concomitant induction of a chromosomally encoded beta-lactamase (ampC). Components of the inducible beta-lactamase system in the enterobacteria have been shown to be required for cell wall recycling. Because sporulation in M. xanthus requires cell wall remodeling, this leads to the hypothesis that components of the inducible beta-lactamase system are required for normal development. The following approach will be taken to test this hypothesis: 1) identify the genes involved in beta-lactamase induction by PCR and transposon mutagenesis, 2) examine regulation of these genes during development by monitoring expression of operon fusions, and 3) analyze biochemically the role of gene products in cell wall remodeling by following turnover of murein components in various mutant backgrounds.

该研究项目的目标是描述诱导剂的作用 黄色粘球菌发育程序中的β-内酰胺酶系统。 最近的证据表明 M.xanthus 将经历饥饿- 独立孢子形成，同时诱导染色体 编码的β-内酰胺酶（ampC）。诱导型β-内酰胺酶的成分 肠细菌中的系统已被证明是细胞壁所必需的 回收。因为 M. xanthus 中的孢子形成需要细胞壁 重塑，这导致了这样的假设：诱导成分 正常发育需要β-内酰胺酶系统。下列 将采取方法来检验这个假设：1）识别基因 通过 PCR 和转座子诱变参与 β-内酰胺酶诱导，2) 通过监测来检查这些基因在发育过程中的调节 操纵子融合体的表达，3) 生化分析 胞壁蛋白周转后细胞壁重塑中的基因产物 各种突变体背景中的成分。