CORE--ADVANCED TECHNOLOGY LABORATORIES

核心--先进技术实验室

• 批准号: 5205325
• 负责人: DOUGLAS D RICHMAN
• 金额: --
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/5205325
• 关键词: AIDS; AIDS therapy; HIV infections; antiAIDS agent; antibody neutralization test; biomarker; biomedical facility; clinical research; cooperative study; cytokine; drug metabolism; human immunodeficiency virus 1; human subject; human therapy evaluation; immunity; immunology; neutralizing antibody; pathologic process; pharmacokinetics; pharmacology; polymerase chain reaction; serology /serodiagnosis; virus antigen

The UCSD Retrovirology Laboratory has made important contributions to the success of the UCSD ACTU and to the retrovirology effort of the overall ACTG program. Some of the accomplishments of the Retrovirology Laboratory have helped to expand the role of virologic markers in clinical trials and to influence the direction of specific ongoing and new ACTG protocols. The Laboratory has worked particularly closely with ACTG investigators participating in Phase I-II studies and has frequently played an important role in helping to guide investigators in future studies. Specimens from both the UCSD Adult and Pediatric ACTUs are processed by the Retrovirology Laboratory which has been consistently certified for HIV isolation and p24 antigen determinations. The Specific Aims of the UCSD Retrovirology Laboratory are: (1) To apply standardized, reproducible and quality- controlled assays of HIV infection as virologic markers in patients participating in ACTG protocols; (2) To provide essential virologic data to help guide clinical investigators in determining future directions of Phase I-II antiretroviral studies; (3) To evaluate the utility of new virologic assays for monitoring the response of patients participating in antiretroviral studies; and (4) To assist in the development of novel virologic assays which can be applied to monitor patients receiving antiretroviral therapy by providing well-characterized and properly stored specimens. Because of the size of the UCSD ACTU, the Retrovirology Laboratory proposes to process annually 500 standard HIV cultures, 350 quantitative cell dilution cultures, 350 plasma viremia cultures, and 24,000 plasma and culture supernatant p24 antigen determinations. The continued participation of the UCSD Retrovirology Laboratory in the UCSD ACTU will help contribute to the development of improved treatments for patients infected with HIV.

UCSD逆转录病毒学实验室已为 UCSD ACTU的成功和整体逆转录病毒学工作 ACTG程序。 逆转录病毒实验室的一些成就 有助于扩大病毒标记在临床试验中的作用， 影响特定持续和新ACTG协议的方向。 这 实验室与ACTG调查人员特别紧密合作 参加I-II期研究，经常发挥重要的作用 帮助指导研究人员将来的研究中的角色。 来自 UCSD成人和小儿ACTU都通过逆转录病毒来处理 实验室始终获得艾滋病毒隔离和p24认证 抗原测定。 UCSD逆转录病毒学的具体目的 实验室是：（1）应用标准化，可重现和质量 - 艾滋病毒感染作为患者的病毒标记的受控测定 参加ACTG协议； （2）提供必需的病毒学数据 帮助指导临床研究人员确定未来的相位方向 I-II抗逆转录病毒研究； （3）评估新病毒学的效用 监测参加患者反应的测定 抗逆转录病毒研究； （4）协助发展新颖 病毒学测定，可用于监测接受的患者 通过提供充分的特征且储存良好的抗逆转录病毒疗法 标本。 由于UCSD ACTU的大小，逆转录病毒学 实验室建议每年处理500种标准艾滋病毒文化，350 定量细胞稀释培养物，350个血浆病毒血症培养物和 24,000个血浆和培养上清液P24抗原测定。 这 UCSD逆转录病毒实验室继续参与UCSD Actu将有助于为改进的治疗方法做出贡献 感染HIV的患者。