MOLECULES MEDIATING LYMPHOCYTE ATTACHMENT

介导淋巴细胞附着的分子

• 批准号: 3176454
• 负责人: MICHAEL D PIERSCHBACHER
• 金额: $ 17.35万
• 依托单位: SANFORD BURNHAM PREBYS MEDICAL DISCOVERY INSTITUTE
• 依托单位国家: 美国
• 财政年份: 1984
• 资助国家: 美国
• 起止时间: 1984-03-15 至 1991-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3176454
• 关键词: T lymphocyte; affinity chromatography; antibody formation; bone marrow; cell adhesion; cell cell interaction; cell differentiation; cell migration; cellular immunity; chemical structure function; collagen; density gradient ultracentrifugation; enzyme linked immunosorbent assay; fibronectins; human subject; immunofluorescence technique; lymphatic tissue; lymphoma; macromolecule; neoplastic cell; radiotracer; thymus; tissue /cell culture

Preliminary data are presented which demonstrate the attachment of lymphoma cells to attachment-promoting molecules such as fibronectin and serum spreading factor. Data are also discussed which indicate that normal lymphocytes from the thymus interact with at least one of these molecules--serum spreading factor. It is proposed here to study the interactions of lymphoid cells with known adhesion molecules. A plan is also presented for the identification of novel molecules involved in mediating the attachment of normal lymphocytes. The fact that normal bone marrow cells attach to a monolayer of thymic epithelium indicates that such molecules exist. These molecules will be examined for structure, function, and tissue distribution. The hypothesis is that the selective attachment of immature lymphocytes to molecules within the thymus probably modulates their differentiation, path of migration, and response to external stimuli. Understanding these interactions will give insight into the behavior and development of lymphocytes as well as possibly provide a better understanding of the behavior of lymphoid tumors. New methods which involve the study of cell attachment to electrophoretically separated proteins are proposed for studying this problem. (LB)

提供了初步数据，证明了淋巴瘤的附着 细胞对附着的促进分子，例如纤连蛋白和血清 扩散因子。 还讨论了数据表明正常的数据 胸腺中的淋巴细胞与至少一种相互作用 分子 - 毛扩散因子。 在这里提议研究 淋巴样细胞与已知粘附分子的相互作用。 一个计划是 还提出了鉴定涉及的新分子 介导正常淋巴细胞的附着。 正常骨的事实 骨髓细胞附着在胸皮上皮的单层上，表明这种 存在分子。 这些分子将检查结构，功能， 和组织分布。 假设是选择性附件 胸腺内未成熟的淋巴细胞可能会调节 它们的区分，迁移路径和对外部的反应 刺激。 了解这些互动将使您深入了解 淋巴细胞的行为和发育以及可能提供的 更好地了解淋巴样肿瘤的行为。 新方法 涉及细胞附着在电泳分离的 提出了用于研究此问题的蛋白质。 （磅）