MULTIDIMENSIONAL SLIT-SCAN PRESCREENING SYSTEM

多维狭缝扫描预筛选系统

• 批准号: 3169308
• 负责人: LEON L WHEELESS
• 金额: $ 22.11万
• 依托单位: UNIVERSITY OF ROCHESTER
• 依托单位国家: 美国
• 财政年份: 1981
• 资助国家: 美国
• 起止时间: 1981-09-01 至 1987-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3169308
• 关键词: biomedical automation; body fluids; cellular oncology; cervical /vaginal smear; cervix neoplasms; clinical biomedical equipment; computer assisted diagnosis; diagnosis quality /standard; early diagnosis; flow cytometry; fluorescent dye /probe; human subject; mass screening; neoplasm /cancer diagnosis; prognosis; tissue /cell preparation; urinary bladder epithelium; urinary tract neoplasms

The goal is to develop an automated prescreening system for gynecological cytology specimens. A multidimensional slit-scan flow system was developed to serve as an automated prescreening instrument for gynecological cytology. A single blind clinical study was carried out to evaluate system performance. Cellular material was collected by scraping the uterine cervix and stained in suspension with acridine orange. We analyzed 856 specimens including 195 abnormal specimens representing a broad spectrum of abnormality. Approximately 50,000 cells were analyzed for each specimen. The system false positive rate was 17%, while the false negative rate was 3.9%. All misclassified abnormals but one, were specimens with cellular changes consistent with a slight dysplasia of nonkeratinizing type. The instrument in its present configuration appeared sensitive to the entire spectrum of abnormality existing in the female genital tract and classified as abnormal any specimen containing on the order of 0.1% (or greater) abnormal cells. An alternate system has been fabricated to provide similar multidimensional slit-scan information from a less complex instrument. This two-station instrument provides a conventional one-dimensional slit-scan measurement station followed by a three-dimensional measurement station that provides a "second look" at any desired cell. Parallel microprocessor contour processing electronics have been fabricated and system testing is in progress. (3)

目的是为妇科开发自动预筛选系统 细胞学标本。 开发了多维裂隙扫描流系统 妇科细胞学的自动预筛选仪器。 一个 进行了盲目的临床研究以评估系统性能。 通过刮子宫颈并染色来收集细胞材料 悬浮在Acridine Orange中。 我们分析了856个标本 195代表广泛异常的异常标本。 每个样品分析了大约50,000个细胞。 系统 误报率为17％，而假负率为3.9％。 全部 错误分类的异常，但一个是带有细胞变化的标本 与非旋转类型的轻微发育不良一致。 乐器 在目前的配置中，似乎对整个光谱敏感 女性生殖道存在异常，并被归类为异常 任何包含0.1％（或更高）异常细胞阶的标本。 已经制造了替代系统，以提供类似的多维 从不太复杂的仪器中缝制信息。 这个两个站 仪器提供常规的一维缝隙扫描测量 车站，然后是一个三维测量站 “第二看”任何所需的单元格。 平行微处理器轮廓 处理电子设备已制造，系统测试已在 进步。 （3）