THE NATURE AND REPAIR OF A NEW FORM OF DNA DAMAGE

一种新形式 DNA 损伤的性质和修复

• 批准号: 3165154
• 负责人: ROSS S FELDBERG
• 金额: $ 12.86万
• 依托单位: TUFTS UNIVERSITY MEDFORD
• 依托单位国家: 美国
• 财政年份: 1976
• 资助国家: 美国
• 起止时间: 1976-06-30 至 1986-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3165154
• 关键词: DNA repair; Escherichia coli; X ray; chemical carcinogen; deToni Fanconi syndrome; density gradient ultracentrifugation; endonuclease; gel electrophoresis; human tissue; nucleic acid structure; oligonucleotides; pyrimidine dimers; radiation genetics; radiation recovery; relative biological effectiveness; tissue /cell culture; ultraviolet radiation; xeroderma pigmentosum

The role of DNA repair in protecting the cell against mutagens and carcinogens has been suggested by a variety of studies. Most of this work, however, has focused exclusively on the cytobutane pyrimidine dimer form of damage in ultraviolet (uv) irradiated DNA. The overall goal of this project is to extend our understanding of the effects of radiation and chemicals on DNA through the study and use of a damage-specific DNA binding protein isolated from human cells. This protein shows a broad specificity for monofunctional base damage in DNA, but does not recognize pyrimidine dimers, apurinic sites, single-strand breaks, single-strand regions or cross-links in DNA. As well as studying the physical and chemical properties of this protein, we have also utilized this protein as a probe to study the introduction of base damage into DNA by ionizing radiation, alkylating agents and a variety of other chemical mutagens. The role of this protein in cellular repair will be elucidated by studying its interaction with DNA-directed enzymes as well as by screening for its presence in cell lines characterized by abnormal sensitivity to radiation.

DNA 修复在保护细胞免受诱变剂和致癌物侵害中的作用 各种研究都提出了这一点。 然而，这项工作的大部分内容 专门关注细胞丁烷嘧啶二聚体形式的损伤 紫外线 (uv) 照射的 DNA。 该项目的总体目标是扩展 我们通过以下方式了解辐射和化学物质对 DNA 的影响 从人体中分离出的损伤特异性 DNA 结合蛋白的研究和使用 细胞。 该蛋白对单功能碱基损伤表现出广泛的特异性 DNA，但不识别嘧啶二聚体、无嘌呤位点、单链 DNA 中的断裂、单链区域或交联。 以及学习 这种蛋白质的物理和化学性质，我们也利用了这个 蛋白质作为探针研究通过电离将碱基损伤引入 DNA 辐射、烷化剂和各种其他化学诱变剂。 角色 该蛋白质在细胞修复中的作用将通过研究其来阐明 与 DNA 定向酶的相互作用以及筛选其存在 在对辐射异常敏感的细胞系中。

项目成果

On the substrate specificity of a damage-specific DNA binding protein from human cells.

人类细胞损伤特异性 DNA 结合蛋白的底物特异性。

• DOI: 10.1093/nar/8.5.1133
• 发表时间: 1980
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Feldberg,RS

Recognition of a cytosine base lesion by a human damage-specific DNA binding protein.

人类损伤特异性 DNA 结合蛋白对胞嘧啶碱基损伤的识别。

• DOI: 10.1093/nar/13.1.303
• 发表时间: 1985
• 期刊: Nucleic acids research
• 影响因子: 14.9
• 作者: Carew,JA;Feldberg,RS
• 通讯作者: Feldberg,RS

Probing photodynamic damage in nucleic acids with a damage-specific DNA binding protein: a comparison of the B and Z DNA conformations.

使用损伤特异性 DNA 结合蛋白探测核酸中的光动力损伤：B 和 Z DNA 构象的比较。

• DOI: 10.1111/j.1751-1097.1983.tb04511.x
• 发表时间: 1983
• 期刊: Photochemistry and photobiology
• 影响因子: 3.3
• 作者: Feldberg,RS;Brown,C;Carew,JA;Lucas,JL
• 通讯作者: Lucas,JL

A damage-specific DNA binding protein. Large scale purification from human placenta and characterization.

一种损伤特异性 DNA 结合蛋白。

• 发表时间: 1982
• 期刊: The Journal of biological chemistry
• 作者: Feldberg,RS;Lucas,JL;Dannenberg,A
• 通讯作者: Dannenberg,A