Investigation of optimal gel conditions for stem cell preservation at room temperature and scaling up of selected methodology

研究室温下保存干细胞的最佳凝胶条件并扩大所选方法的规模

基本信息

批准号：
BB/K011111/2
负责人：
Che Connon
金额：
$ 57.51万
依托单位：
Newcastle University
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2014
资助国家：
英国
起止时间：
2014 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FK011111%2F2
关键词：
Investigation optimal gel conditions stem

项目摘要

For numerous reasons (financial, resource, scientific etc) the centres of excellence responsible for the preparation/manufacture (cell banking, growth, culture, purity and QA etc) of the Living Biologic (LB) in any Regenerative Therapeutic (RT) are geographically separated to that of the point of use. Therefore, a major challenge is to deliver to the clinic the LB in a validated, quality, 'fit for purpose' form. Across the global market place there is a pressing need to retain the key LB characteristic for at least several days to one week. Although suitable for longer term banking, Cryofreezing for the final product is not an option due to the acknowledged negative effects on the LB. As important, the system needs to be simple, rapid and easy to use at the point of care in order to ease uptake by the clinician. Our recent BRIC enabling grant has shown that an alginate gel cell encapsulation system meets many of these needs. We have a growing body of evidence that demonstrates it is effective at preserving a number of cell types (including stem cells) over extended periods in room temperature conditions. However, further research is needed to fully understand how it works and demonstrate that the technology can be implemented in an industrial setting (i.e. scalable).

出于许多原因（财务，资源，科学等），在任何再生治疗（RT）中，负责制备/制造（细胞库，生长，文化，纯度和质量质量等）的卓越中心（细胞库，生长，文化，纯度和质量质量等）都是地理位置上的分开到使用点。因此，一个主要的挑战是以经过验证的质量“适合目的”形式将LB送到诊所。在整个全球市场中，迫切需要将关键LB特性保留至少几天到一周。尽管适用于长期银行业务，但由于对LB的承认负面影响，最终产品的冷冻冻结尚无法选择。重要的是，该系统必须在护理点上简单，快速且易于使用，以便减轻临床医生的吸收。我们最近的新金砖化授予赠款表明，藻酸盐凝胶细胞封装系统满足了许多需求。我们越来越多的证据证明它在室温条件下长时间保存多种细胞类型（包括干细胞）有效。但是，需要进一步的研究以充分了解其工作原理，并证明该技术可以在工业环境（即可扩展）中实施。

项目成果

期刊论文数量（7）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Alginate hydrogels for the 3D culture and therapeutic delivery of cells

用于细胞 3D 培养和治疗递送的藻酸盐水凝胶

DOI：
发表时间：
2014
期刊：
RSC Soft Matter
影响因子：
0
作者：
Wright B.
通讯作者：
Wright B.

Oxidized alginate hydrogels as niche environments for corneal epithelial cells.

DOI：
10.1002/jbm.a.35011
发表时间：
2014-10
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A
影响因子：
4.9
作者：
Wright, Bernice;De Bank, Paul A.;Luetchford, Kim A.;Acosta, Fernando R.;Connon, Che J.
通讯作者：
Connon, Che J.

Keeping cells in their place: the future of stem cell encapsulation.

将细胞保持在适当的位置：干细胞封装的未来。