PROLIFERATION AND DIFFERENTIATION OF STEM CELLS

干细胞的增殖和分化

• 批准号: 3163409
• 负责人: PETER M MAUCH
• 金额: $ 40.98万
• 依托单位: HARVARD MEDICAL SCHOOL
• 依托单位国家: 美国
• 财政年份: 1977
• 资助国家: 美国
• 起止时间: 1977-09-01 至 1987-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3163409
• 关键词: Hodgkin's disease; X ray; bleomycin; bone marrow transplantation; cell death; cell differentiation; cellular oncology; combination cancer therapy; cyclophosphamide; drug adverse effect; erythrocytes; erythropoiesis; graft versus host disease; hematopoiesis; hematopoietic growth factor; hematopoietic stem cells; human subject; immunofluorescence technique; leukocyte activation /transformation; lymphocyte; methotrexate; myeloid stem cell; myeloproliferative neoplasm; neoplasm /cancer chemotherapy; neoplasm /cancer immunotherapy; neoplasm /cancer radiation therapy; nitrogen mustard; procarbazine; radiation dosage; radiation therapy dosage; radiobiology; radiotracer; relative biological effectiveness; scintillation counter; synchronous cell division; tissue /cell culture; tumor antigens; vinblastine

The bone marrow is an important dose limiting cell renewal tissue for both chemotherapy and wide field irradiation. The purpose of this project is to study the stem cell compartment of the bone marrow; specifically its organization and what implications its organization has in the clinical use of chemotherapeutic agents and irradiation. We shall test our theory of stem cell organization as it relates to late effects of chemotherapy and to bone marrow transplantation. We shall study the use of in vitro techniques to best prepare bone marrow for transplantation while avoiding graft versus host disease. We shall determine whether we can use the in vitro technique to develop functional marrow made resistant to chemotherapeutic agents. We shall study the best mechanism of preparing the host for bone marrow transplantation to reduce the toxicity of such treatment yet not limit its efficacy. These projects have obvious clinical implications in cancer chemotherapy, radiation therapy and bone marrow transplantation.

骨髓是两者的重要剂量限制细胞更新组织 化学疗法和广泛的辐射。 这个项目的目的是 研究骨髓的干细胞室；特别是它的 组织以及其组织对临床使用的影响 化学治疗剂和辐照。 我们将测试我们的理论 干细胞组织与化学疗法的后期作用有关， 骨髓移植。 我们将研究体外技术的使用 最好准备骨髓进行移植，同时避免移植 宿主病。 我们将确定是否可以使用体外技术 为了发展功能性并且从化学治疗剂具有抗性。 我们 应研究为骨髓准备宿主的最佳机制 移植以降低这种治疗的毒性，但不限制 功效。 这些项目对癌症具有明显的临床意义 化学疗法，放射治疗和骨髓移植。