SUMO proteomics in C. elegans; germ line, meiosis and the DNA damage response
线虫中的相扑蛋白质组学;
基本信息
- 批准号:BB/J015199/1
- 负责人:
- 金额:$ 38.62万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2013
- 资助国家:英国
- 起止时间:2013 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Proteins need to be regulated to properly fulfil their function in cells and living organisms. Specialized sets of proteins are required to convey a variety of signals within living cells. Such signalling proteins confer signals by attaching distinct sets of molecules to their target proteins. The attachment of such molecules, referred to as modification serves as a 'cellular language'. Modern molecular biology research focuses on such signalling proteins and tries to address their importance in modifying the activity of their targets. Modifications that confer signals such as phosphorylation or ubiquitylation have been known for a long time. However, only in the past decade, it became clear that sumoylation provides an important way of intracellular communication. The Hay laboratory elaborated important molecular details pertaining to basic biochemical reactions that are needed for SUMO modification. SUMO by itself is a sizable protein, that is attached to certain residues of its target proteins and several proteins are needed to confer sumoylation. So called 'systems approaches' aim to provide a comprehensive analysis and understanding of the 'SUMO language'. Such analysis requires the identification, of all SUMO modified proteins, and a detailed analysis of how and to which extend SUMO modification changes in response to various physiological and pathophysiological conditions. First experiments to assess the global role of SUMO modification were done in with cell cultures. While those systems have obvious experimental advantages, these cells do not necessarily reflect the behaviour of cells in intact organisms. This is why we plan to use the small roundworm C. elegans as a model system to globally asses the extend, and importance of sumoylation in a living organism. C. elegans was chosen as the simplest possible animal system. Studies are facilitated by the simplicity of the organism at the developmental and anatomical level, by the ease of its maintenance, as well as by the availability of methods that allow for manipulating its genetic information procedures necessary to study the importance of SUMO modification. Despite of its simplicity, C. elegans is a multicellular organism that shares many fundamental genetic programs with humans. Thus results obtained in the C. elegans system are likely to be applicable to humans and working with worms does not raise ethical concerns. As part of our studies we want to express modified versions of SUMO in the worm, such that we can best apply the advanced methods we have developed to isolate all SUMO modified proteins. To identify those proteins, but also to assess the extent of SUMO modification we will use a method we recently developed allowing for the identification and quantification of thousands of worm proteins. We not only want to find all SUMO modified proteins, but also want to assess the importance of SUMO modification. To start with this, we chose an important biological processes extensively studied in our labs. 1) Meiosis is a specialised cell division, needed for the generation of germ cells. During this division the duplicated set of chromosomes occurring in normal cells is reduced to just one copy. The subsequent fusion of germ cell restores a duplicated set of chromosomes one coming from the father, another one coming from the mother. Not only this, during meiosis a process referred to as recombination also mixes individual chromosomes such that chromosomes contain elements from both grandparents. Recombination ensures genetic diversity. 2) Recombination pertains to the breaking and linking DNA molecules. Recombination is used when DNA damaging agents such as ionizing irradiation breaks chromosomes. We already have know that SUMO modification is important for meiosis and recombination and we now want perform a systematic analysis of this. Our studies are important to understand how the genetic information is passed on from one generation to the next.
Proteins need to be regulated to properly fulfil their function in cells and living organisms. Specialized sets of proteins are required to convey a variety of signals within living cells. Such signalling proteins confer signals by attaching distinct sets of molecules to their target proteins. The attachment of such molecules, referred to as modification serves as a 'cellular language'.现代分子生物学研究的重点是这种信号蛋白,并试图解决它们在修改目标活性方面的重要性。 Modifications that confer signals such as phosphorylation or ubiquitylation have been known for a long time. However, only in the past decade, it became clear that sumoylation provides an important way of intracellular communication. The Hay laboratory elaborated important molecular details pertaining to basic biochemical reactions that are needed for SUMO modification. Sumo本身是一种相当大的蛋白质,它附着在其靶蛋白的某些残基上,需要几种蛋白来赋予Sumoylation。 So called 'systems approaches' aim to provide a comprehensive analysis and understanding of the 'SUMO language'.这样的分析需要对所有SUMO修饰蛋白的识别,以及详细的分析,分析如何以及向Sumo修饰的变化而变化,以响应各种生理和病理生理条件。 First experiments to assess the global role of SUMO modification were done in with cell cultures. While those systems have obvious experimental advantages, these cells do not necessarily reflect the behaviour of cells in intact organisms.这就是为什么我们计划将小round虫秀丽隐杆线虫作为模型系统,以在全球范围内扩展和在活生物体中的sumoylation的重要性。 C. elegans was chosen as the simplest possible animal system.由于生物体在发育和解剖学水平上的简单性,通过其维护的简化以及允许操纵其基因信息修改重要性所必需的遗传信息程序的可用性来促进研究。 Despite of its simplicity, C. elegans is a multicellular organism that shares many fundamental genetic programs with humans. Thus results obtained in the C. elegans system are likely to be applicable to humans and working with worms does not raise ethical concerns.作为研究的一部分,我们希望在蠕虫中表达Sumo的修改版本,以便我们可以最好地应用我们开发的先进方法来隔离所有Sumo修饰的蛋白质。为了识别这些蛋白质,但为了评估Sumo修饰的程度,我们将使用最近开发的方法,允许识别和定量数千种蠕虫蛋白。我们不仅想找到所有Sumo修饰的蛋白质,而且还希望评估Sumo修饰的重要性。首先,我们选择了在实验室中广泛研究的重要生物学过程。 1)减数分裂是一种专门的细胞分裂,需要生成生殖细胞。在此划分期间,在正常细胞中发生的重复的染色体将减少为仅一个拷贝。随后的生殖细胞融合恢复了一套重复的染色体,其中一个来自父亲,另一个来自母亲。不仅如此,在减数分裂过程中,称为重组的过程还将单个染色体混合在一起,因此染色体含有两个祖父母的元素。重组确保遗传多样性。 2)重组与断裂和连接DNA分子有关。当DNA损坏剂(例如电离辐射断裂)染色体时,使用重组。我们已经知道,Sumo修饰对于减数分裂和重组很重要,现在我们希望对此进行系统分析。我们的研究对于了解如何从一代传递到另一代的遗传信息很重要。
项目成果
期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
The SMC-5/6 Complex and the HIM-6 (BLM) Helicase Synergistically Promote Meiotic Recombination Intermediate Processing and Chromosome Maturation during Caenorhabditis elegans Meiosis.
- DOI:10.1371/journal.pgen.1005872
- 发表时间:2016-03
- 期刊:
- 影响因子:4.5
- 作者:Hong Y;Sonneville R;Agostinho A;Meier B;Wang B;Blow JJ;Gartner A
- 通讯作者:Gartner A
LEM-3 is a midbody-tethered DNA nuclease that resolves chromatin bridges during late mitosis.
- DOI:10.1038/s41467-018-03135-w
- 发表时间:2018-02-20
- 期刊:
- 影响因子:16.6
- 作者:Hong Y;Sonneville R;Wang B;Scheidt V;Meier B;Woglar A;Demetriou S;Labib K;Jantsch V;Gartner A
- 通讯作者:Gartner A
Erratum: Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans.
- DOI:10.1038/ncomms7352
- 发表时间:2015-02-12
- 期刊:
- 影响因子:16.6
- 作者:Pelisch, Federico;Sonneville, Remi;Pourkarimi, Ehsan;Agostinho, Ana;Blow, J. Julian;Gartner, Anton;Hay, Ronald T.
- 通讯作者:Hay, Ronald T.
Dynamic SUMO modification regulates mitotic chromosome assembly and cell cycle progression in Caenorhabditis elegans.
- DOI:10.1038/ncomms6485
- 发表时间:2014-12-05
- 期刊:
- 影响因子:16.6
- 作者:Pelisch, Federico;Sonneville, Remi;Pourkarimi, Ehsan;Agostinho, Ana;Blow, J. Julian;Gartner, Anton;Hay, Ronald T.
- 通讯作者:Hay, Ronald T.
A SUMO-Dependent Protein Network Regulates Chromosome Congression during Oocyte Meiosis.
- DOI:10.1016/j.molcel.2016.11.001
- 发表时间:2017-01-05
- 期刊:
- 影响因子:16
- 作者:Pelisch F;Tammsalu T;Wang B;Jaffray EG;Gartner A;Hay RT
- 通讯作者:Hay RT
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Anton Gartner其他文献
signatures related to carcinogens and DNA repair deficiency whole genome sequencing reveals mutational
与致癌物和 DNA 修复缺陷相关的特征全基因组测序揭示了突变
- DOI:
- 发表时间:
2014 - 期刊:
- 影响因子:0
- 作者:
S. Cooke;Joerg Weiss;A. Bailly;L. Alexandrov;J. Marshall;K. Raine;M. Maddison;Elizabeth Anderson;Michael R. Stratton;Anton Gartner;Peter J. Campbell - 通讯作者:
Peter J. Campbell
DETEKSI AFLATOKSIN B 1 PADA JENIS MAKANAN OLAHAN JAGUNG MENGGUNAKAN ENZYME-LINKED IMMUNOSORBENT ASSAY ( ELISA ) Detection of Aflatoxin B 1 in Processed Foods Corn by Enzyme-Linked Immunosorbent Assay ( ELISA )
DETEKSI AFLATOKSIN B 1 PADA JENIS MAKANAN OLAHAN JAGUNG MENGGUNAKAN 酶联免疫吸附测定 ( ELISA ) 通过酶联免疫吸附测定 ( ELISA ) 检测加工食品玉米中的黄曲霉毒素 B 1
- DOI:
- 发表时间:
2015 - 期刊:
- 影响因子:0
- 作者:
S. Cooke;Joerg Weiss;A. Bailly;L. Alexandrov;J. Marshall;K. Raine;M. Maddison;Elizabeth Anderson;Michael R. Stratton;Anton Gartner;Peter J. Campbell - 通讯作者:
Peter J. Campbell
Tardigrades: Trained to be hardy in the face of DNA damage
缓步动物:经过训练,能够抵御 DNA 损伤
- DOI:
10.1016/j.cub.2024.04.030 - 发表时间:
2024 - 期刊:
- 影响因子:9.2
- 作者:
Stéphane G.M. Rolland;N. Memar;Anton Gartner - 通讯作者:
Anton Gartner
Analysis of mutational signatures in <em>C. elegans</em>: Implications for cancer genome analysis
- DOI:
10.1016/j.dnarep.2020.102957 - 发表时间:
2020-11-01 - 期刊:
- 影响因子:
- 作者:
Bettina Meier;Nadezda V Volkova;Moritz Gerstung;Anton Gartner - 通讯作者:
Anton Gartner
Anton Gartner的其他文献
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