Emerging Chlamydia-like organisms as novel causes of bovine reproductive failure

新兴的衣原体类生物体是牛繁殖失败的新原因

基本信息

批准号：
BB/J015083/1
负责人：
David Longbottom
金额：
$ 78.34万
依托单位：
Moredun Research Institute
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2013
资助国家：
英国
起止时间：
2013 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FJ015083%2F1
关键词：
Emerging Chlamydia like organisms novel

项目摘要

Many infectious diseases affect livestock, impacting not only on the health and welfare of the animals but also on the economic sustainability of the agricultural industry and future food security. Reproductive failure in cattle is one area of great concern to the agricultural sector, as it has a major impact on productivity in UK cattle herds. While there are many factors contributing to reduced rates of reproduction in livestock systems, infection plays a key role, with 77% of diagnosed cases of bovine fetal death reported as resulting from infectious causes. However, diagnosis of the infectious causes of pre-natal death in cattle is poor, with 80% of cases remaining undiagnosed (according to DEFRA's Veterinary Investigation Surveillance reports for 2002-09). This can be explained in part by a failure to detect the presence of other unidentified disease causing organisms.In recent years, there has been an increase in the identification of a group of new emerging bacterial organisms that are found in the environment and have been shown to be associated with a variety of conditions in humans, such as pneumonia and miscarriage. These organisms, which share similar biological characteristics to Chlamydia species that are known to cause a broad range of infections in humans and animals, such as sexually-transmitted infections, pneumonia, blindness and fetal death, are referred to as Chlamydia-like organisms. These Chlamydia-like organisms are also increasingly becoming recognized as potential disease causing organisms of livestock, being particularly associated with the pre-natal death of calves. Indeed, they have been found in over a quarter of the cases analyzed in the UK, and thus could account for some of the 80% unaccounted, undiagnosed cases reported by DEFRA.To date, the only studies that have been carried out have relied upon the analysis of tissue samples, which have been submitted to veterinary laboratories for disease diagnosis. While these studies have been vital in demonstrating the presence of the organisms in samples for which no alternative diagnoses could be reached, they have been performed on a relatively small number of samples. In addition, in general, little information is obtained on the disease and production histories of the farms from which the animals originated. Thus, this study aims to investigate the presence of these organisms on dairy farms across the UK and how this relates to animal production performance. We will isolate the organisms from clinical samples to allow us to characterise and assess how many different types of Chlamydia-like organisms are present in the UK cattle herds, as well as investigate their potential spread from animal to animal by analyzing environmental samples, such as drinking water and bedding. We will also develop experimental model systems to allow us to investigate how the organisms cause infection and disease, and determine the immune response to infection.The combination of these studies will greatly increase our understanding of the disease causing potential and role of this group of emerging bacteria in cattle reproduction. The outcomes will lead to improved diagnoses of cattle reproductive failure, inform and educate the industry to the presence of these organisms, lead to improved management systems and allow an evaluation of the potential benefits of future vaccine strategies to prevent disease incidence.

许多传染病影响牲畜，不仅影响动物的健康和福利，还影响农业的经济可持续性和未来的粮食安全。牛的繁殖障碍是农业部门高度关注的领域之一，因为它对英国牛群的生产力产生重大影响。虽然导致畜牧系统繁殖率降低的因素有很多，但感染起着关键作用，据报告，77% 的牛胎死亡确诊病例是由感染原因造成的。然而，对牛产前死亡感染原因的诊断很差，80%的病例仍未确诊（根据 DEFRA 2002-09 年兽医调查监测报告）。这可以部分解释为未能检测到其他不明致病生物体的存在。近年来，对环境中发现的一组新出现的细菌生物体的识别有所增加，这些细菌生物体已被证明与人类的多种疾病有关，例如肺炎和流产。这些生物体与已知会引起人类和动物广泛感染（例如性传播感染、肺炎、失明和胎儿死亡）的衣原体物种具有相似的生物学特征，被称为类衣原体生物体。这些衣原体样生物体也越来越被认为是潜在的导致牲畜疾病的生物体，尤其与小牛的产前死亡有关。事实上，在英国超过四分之一的分析病例中都发现了它们，因此 DEFRA 报告的 80% 下落不明、未确诊病例中可能有一部分是由它们引起的。迄今为止，唯一进行的研究依赖于对组织样本进行分析，并将其提交给兽医实验室进行疾病诊断。虽然这些研究对于证明无法进行替代诊断的样本中存在微生物至关重要，但它们是在相对较少的样本上进行的。此外，一般来说，关于动物来源农场的疾病和生产历史的信息很少。因此，本研究旨在调查英国各地奶牛场中这些生物体的存在情况以及其与动物生产性能的关系。我们将从临床样本中分离出这些生物体，以便我们能够表征和评估英国牛群中存在多少种不同类型的衣原体样生物体，并通过分析环境样本来调查它们在动物之间的潜在传播，例如饮用水和床上用品。我们还将开发实验模型系统，使我们能够研究生物体如何引起感染和疾病，并确定对感染的免疫反应。这些研究的结合将大大增加我们对这组新兴细菌的致病潜力和作用的理解。牛繁殖中的细菌。研究结果将改进对牛繁殖失败的诊断，向行业通报和教育这些生物体的存在，改进管理系统，并允许评估未来预防疾病发生的疫苗策略的潜在益处。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Pathogenesis of Greek variant strains of Chlamydia abortus in sheep

羊流产衣原体希腊变异株的发病机制

DOI：
发表时间：
2013
期刊：
影响因子：
0
作者：
Longbottom D
通讯作者：
Longbottom D

Genotypic identification of the Chlamydia abortus vaccine strain in the placentas of aborted sheep vaccinated against ovine enzootic abortion

羊地方性流产疫苗流产羊胎盘流产衣原体疫苗株的基因型鉴定

DOI：
发表时间：
2013
期刊：
影响因子：
0
作者：
Livingstone M
通讯作者：
Livingstone M

Occurence of chlamydial 16S rDNA sequences in environmental sediment samples and in nasal swab material from grey seal (Halichoreus grypus) pups on the Isle of May in Scotland

环境沉积物样本和苏格兰五月岛灰海豹 (Halichoreus grypus) 幼崽的鼻拭子材料中存在衣原体 16S rDNA 序列

DOI：
发表时间：
2015
期刊：
影响因子：
0
作者：
Flockhart A
通讯作者：
Flockhart A

Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS.

DOI：
10.1371/journal.pone.0177653
发表时间：
2017
期刊：
PloS one
影响因子：
3.7
作者：
Livingstone M;Wheelhouse N;Ensor H;Rocchi M;Maley S;Aitchison K;Wattegedera S;Wilson K;Sait M;Siarkou V;Vretou E;Entrican G;Dagleish M;Longbottom D
通讯作者：
Longbottom D

Presence of DNA from Chlamydia-like organisms in the nasal cavities of grey seal pups (Halichoerus grypus) and three different substrates present in a breeding colony.