Integrated genomic and proteomic characterisation of autotransporter proteins of obligate intracellular bacteria C. abortus and L. intracellularis
专性胞内细菌 C. abortus 和 L. intracellularis 的自转运蛋白的综合基因组和蛋白质组学特征
基本信息
- 批准号:BB/E018939/1
- 负责人:
- 金额:$ 181.81万
- 依托单位:
- 依托单位国家:英国
- 项目类别:Research Grant
- 财政年份:2008
- 资助国家:英国
- 起止时间:2008 至 无数据
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Many infectious diseases affect livestock and impact not only on the health and welfare of animals but also on the economic sustainability of our agriculture industry. Among important animal pathogens are some bacteria that live within and are entirely dependent upon host cells. These so-called 'obligate intracellular' bacteria are very difficult to work with hence gaining understanding to aid in improving animal health is very problematic. Since this type of pathogen must infect a host animal to survive they typically develop mechanisms to very efficiently enter and take over host cells and evade the host's defences. Among the obligate intracellular bacteria, Chlamydophila abortus and Lawsonia intracellularis are very highly prevalent in sheep and pigs, respectively. Since such a high proportion of animals are affected, these infections have a considerable effect on animal health, quality of life and productivity. Although vaccines to these pathogens are available these have shortcomings so ultimately, we would like to develop efficient methods to very effectively reduce the effects of these diseases. Since these bacteria are difficult to work with, use of high-technology genomic methods can be very cost-effective and productive ways to improve the understanding of components with important roles in infection and make new developments in controlling disease. Genome sequences provide a wealth of information about pathogens and from this information we have identified a family of components - so-called Type V secretion proteins - which are likely to be involved in causing disease. Analysis of genomes is very informative however this is of greatest value when combined with an understanding of the function of the protein products of genes. We intend to study this family of proteins using computer-based approaches and laboratory methodologies to gain comprehension of these Type V proteins. These proteins are present on the surface of bacteria and, typically, are recognised by host defences and can result in the host activating defences that protect against infection. The ability to vary these proteins can be an important way to 'camouflage' pathogens allowing them to escape from host defences. Both C. abortus and L. intracellularis have many members of these Type V proteins thus we consider it is very likely that the members of this family present on the bacteria surface will vary. By gaining an understanding of how the presence of these proteins varies in each of these species we will be in a position to define the family members that are most important to infection and therefore lead to improved detection, treatment or prevention of these important livestock diseases.
许多传染病不仅会影响牲畜和影响动物的健康和福利,还影响我们的农业行业的经济可持续性。重要的动物病原体中有一些生活在内部并完全取决于宿主细胞的细菌。这些所谓的“细胞内”细菌很难与获得理解以帮助改善动物健康的理解非常有问题。由于这种类型的病原体必须感染宿主动物以生存,因此通常会开发机制,以非常有效地进入和接管宿主细胞并逃避宿主的防御能力。在强制性细胞内细菌中,毛细血管菌和劳森氏菌分别在绵羊和猪中非常普遍。由于影响很高的动物受到影响,因此这些感染对动物健康,生活质量和生产力产生了相当大的影响。尽管可以使用这些病原体的疫苗,但最终存在这些缺点,我们希望开发有效的方法以非常有效地减少这些疾病的影响。由于这些细菌很难与之作用,因此使用高科技基因组方法可能是非常成本效益且有效的方法,可以提高对感染中重要作用的成分的理解,并在控制疾病中发展新的发展。基因组序列提供了有关病原体的大量信息,从这些信息中,我们已经确定了一个成分家族 - 所谓的V型分泌蛋白 - 可能与引起疾病有关。基因组的分析非常有用,但是当了解基因蛋白质产物的功能时,这是最大的值。我们打算使用基于计算机的方法和实验室方法来研究这种蛋白质家族,以了解V型蛋白质。这些蛋白质存在于细菌的表面上,通常是通过宿主防御识别的,可以导致宿主激活防御剂,以防止感染。改变这些蛋白质的能力可能是“伪装”病原体的重要方法,使其可以摆脱宿主防御能力。 abortus c. c. c.c。ellicellularis都有许多V型蛋白质中的许多成员,因此我们认为,存在于细菌表面上的该家族的成员很可能会有所不同。通过了解这些蛋白质的存在如何在这些物种中有何不同,我们将有能力定义对感染最重要的家庭成员,从而改善了检测,治疗或预防这些重要的牲畜疾病。
项目成果
期刊论文数量(10)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Therapeutics and Control of Sheep and Goat Diseases, An Issue of Veterinary Clinics: Food Animal Practice
绵羊和山羊疾病的治疗和控制,兽医诊所的一个问题:食用动物实践
- DOI:
- 发表时间:2011
- 期刊:
- 影响因子:0
- 作者:Fthenakis, George C.;Menzies, Paula
- 通讯作者:Menzies, Paula
RFLP-analysis of polymorphic outer membrane protein loci reveals intraspecies-specific variability among Chlamydophila abortus isolates
多态性外膜蛋白位点的 RFLP 分析揭示了流产衣原体分离株的种内特异性变异
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:Ewan Clark (Author)
- 通讯作者:Ewan Clark (Author)
Evidence of the association of Chlamydia-like organisms with cases of bovine abortion in the UK
类衣原体生物与英国牛流产病例相关的证据
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:David Longbottom (Author)
- 通讯作者:David Longbottom (Author)
Profiling antibody responses to infections by Chlamydia abortus enables identification of potential virulence factors and candidates for serodiagnosis.
- DOI:10.1371/journal.pone.0080310
- 发表时间:2013
- 期刊:
- 影响因子:3.7
- 作者:Forsbach-Birk V;Foddis C;Simnacher U;Wilkat M;Longbottom D;Walder G;Benesch C;Ganter M;Sachse K;Essig A
- 通讯作者:Essig A
Exposure to viral and bacterial pathogens among Soay sheep (Ovis aries) of the St Kilda archipelago.
- DOI:10.1017/s0950268816000017
- 发表时间:2016-07
- 期刊:
- 影响因子:4.2
- 作者:Graham AL;Nussey DH;Lloyd-Smith JO;Longbottom D;Maley M;Pemberton JM;Pilkington JG;Prager KC;Smith L;Watt KA;Wilson K;McNEILLY TN;Brülisauer F
- 通讯作者:Brülisauer F
共 6 条
- 1
- 2
David Longbottom其他文献
Subunit structure of calgranulins A and B obtained from sputum, plasma, granulocytes and cultured epithelial cells
- DOI:10.1016/0167-4838(92)90273-g10.1016/0167-4838(92)90273-g
- 发表时间:1992-04-081992-04-08
- 期刊:
- 影响因子:
- 作者:David Longbottom;Jean-Michel Sallenave;Veronica van HeyningenDavid Longbottom;Jean-Michel Sallenave;Veronica van Heyningen
- 通讯作者:Veronica van HeyningenVeronica van Heyningen
Edinburgh Research Explorer Presence of DNA from Chlamydia-like organisms in the nasal cavities of grey seal pups (Halichoerus grypus) and three different substrates present in a breeding colony
爱丁堡研究探索者灰海豹幼崽(Halichoerus grypus)鼻腔中存在类衣原体生物的 DNA 以及繁殖群体中存在的三种不同底物
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:M. P. Dagleish;A. Flockhart;J. Baily;Ailsa J. Hall;T. Simpson;David LongbottomM. P. Dagleish;A. Flockhart;J. Baily;Ailsa J. Hall;T. Simpson;David Longbottom
- 通讯作者:David LongbottomDavid Longbottom
Single channel analysis of recombinant major outer membrane protein porins from Chlamydia psittaci and Chlamydia pneumoniae
鹦鹉热衣原体和肺炎衣原体重组主要外膜蛋白孔蛋白的单通道分析
- DOI:10.1016/s0014-5793(99)00121-010.1016/s0014-5793(99)00121-0
- 发表时间:19991999
- 期刊:
- 影响因子:3.5
- 作者:Susan Wyllie;David Longbottom;A. J. Herring;Richard H. AshleySusan Wyllie;David Longbottom;A. J. Herring;Richard H. Ashley
- 通讯作者:Richard H. AshleyRichard H. Ashley
Identification of a multigene family coding for the 90 kDa proteins of the ovine abortion subtype of Chlamydia psittaci.
编码鹦鹉热衣原体绵羊流产亚型 90 kDa 蛋白的多基因家族的鉴定。
- DOI:
- 发表时间:19961996
- 期刊:
- 影响因子:2.1
- 作者:David Longbottom;Mary Russell;Gareth E. Jones;F. Lainson;A. J. HerringDavid Longbottom;Mary Russell;Gareth E. Jones;F. Lainson;A. J. Herring
- 通讯作者:A. J. HerringA. J. Herring
Inflammatory cytokine responses in a pregnant mouse model of <em>Chlamydophila abortus</em> infection
- DOI:10.1016/j.vetmic.2010.01.02510.1016/j.vetmic.2010.01.025
- 发表时间:2010-08-262010-08-26
- 期刊:
- 影响因子:
- 作者:Karen Kerr;Nicholas Wheelhouse;Morag Livingstone;Ian E. Anderson;Gary Entrican;Declan McKeever;David LongbottomKaren Kerr;Nicholas Wheelhouse;Morag Livingstone;Ian E. Anderson;Gary Entrican;Declan McKeever;David Longbottom
- 通讯作者:David LongbottomDavid Longbottom
共 5 条
- 1
David Longbottom的其他基金
Biological and pathological characterisation of novel plasmid-carrying avian Chlamydia abortus strain 84/2334
新型质粒携带禽流产衣原体菌株 84/2334 的生物学和病理学特征
- 批准号:BB/X016692/1BB/X016692/1
- 财政年份:2023
- 资助金额:$ 181.81万$ 181.81万
- 项目类别:Research GrantResearch Grant
Emerging Chlamydia-like organisms as novel causes of bovine reproductive failure
新兴的衣原体类生物体是牛繁殖失败的新原因
- 批准号:BB/J015083/1BB/J015083/1
- 财政年份:2013
- 资助金额:$ 181.81万$ 181.81万
- 项目类别:Research GrantResearch Grant
相似国自然基金
基于跨组学数据研究质体基因组中非同义突变对蛋白质结构的影响
- 批准号:32300539
- 批准年份:2023
- 资助金额:30 万元
- 项目类别:青年科学基金项目
大规模多组学数据识别人类基因组编码的暗蛋白质
- 批准号:
- 批准年份:2022
- 资助金额:30 万元
- 项目类别:青年科学基金项目
大规模多组学数据识别人类基因组编码的暗蛋白质
- 批准号:32200528
- 批准年份:2022
- 资助金额:30.00 万元
- 项目类别:青年科学基金项目
肾透明细胞癌新抗原的蛋白质基因组学筛选及其在免疫应答过程中机制研究
- 批准号:
- 批准年份:2022
- 资助金额:30 万元
- 项目类别:青年科学基金项目
肾透明细胞癌新抗原的蛋白质基因组学筛选及其在免疫应答过程中机制研究
- 批准号:32201215
- 批准年份:2022
- 资助金额:30.00 万元
- 项目类别:青年科学基金项目
相似海外基金
Risk prediction of heart failure using an integrated genomic-proteomic approach
使用集成基因组-蛋白质组学方法预测心力衰竭的风险
- 批准号:418971418971
- 财政年份:2020
- 资助金额:$ 181.81万$ 181.81万
- 项目类别:Operating GrantsOperating Grants
Risk prediction of cardio-metabolic diseases using an integrated genomic-proteomic approach
使用集成基因组-蛋白质组学方法预测心脏代谢疾病的风险
- 批准号:380342380342
- 财政年份:2018
- 资助金额:$ 181.81万$ 181.81万
- 项目类别:Operating GrantsOperating Grants
Proteomic-based integrated subject-specific networks in cancer
癌症中基于蛋白质组学的综合主题特定网络
- 批准号:95060279506027
- 财政年份:2018
- 资助金额:$ 181.81万$ 181.81万
- 项目类别:
An Integrated Genomic, Proteomic and Cytological Approach to Understand the Function of Cytoplasmic Localized, Cytoskeletal-Associated RNA-Binding Proteins in Rice
一种综合基因组学、蛋白质组学和细胞学方法来了解水稻细胞质定位、细胞骨架相关 RNA 结合蛋白的功能
- 批准号:06050160605016
- 财政年份:2006
- 资助金额:$ 181.81万$ 181.81万
- 项目类别:Continuing GrantContinuing Grant
The molecular basis of cold adaptation: an integrated genomic and proteomic study of Antarctic archaea.
冷适应的分子基础:南极古菌的基因组和蛋白质组学综合研究。
- 批准号:DP0662883DP0662883
- 财政年份:2006
- 资助金额:$ 181.81万$ 181.81万
- 项目类别:Discovery ProjectsDiscovery Projects