MOLECULAR GENETIC ANALYSIS OF T. CRUZI NEURAMINIDASE

T. Cruzi 神经氨酸酶的分子遗传学分析

• 批准号: 3134748
• 负责人: MAGDALENE Y SO
• 金额: $ 10.58万
• 依托单位: SCRIPPS CLINIC AND RESEARCH FOUNDATION
• 依托单位国家: 美国
• 财政年份: 1986
• 资助国家: 美国
• 起止时间: 1986-01-01 至 1988-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3134748
• 关键词: Trypanosoma cruzi; exo alpha sialidase; gel electrophoresis; gene expression; genetic manipulation; genetic regulation; genetic strain; high performance liquid chromatography; life cycle; microorganism genetics; molecular biology; molecular pathology; tropical medicine; trypanosomiasis; virulence

Many protozoa which are members of the Kinetoplastida family have complicated life cycle involving several stages of morphogenesis. To date little is known about the genetic regulation of expression of stage specific genes in these microorganisms or the contribution each stage specific surface protein makes to the biology of the organism (with the exception of the VSG genes of Trypanosoma brucei). The neuraminidase is a developmentally regulated enzyme in Trypanosoma cruzi and a possible virulence factor. We will use a combination of recombinant DNA technology, protein biochemistry, and immunological techniques to characterize the enzyme and to study the regulatory mechanism governing the expression of the gene. Our experiments will serve as a model for future studies aimed at identifying the components important for intracellular survival of this organism.

许多原生动物是Kinetoplastida家族的成员 复杂的生命周期涉及形态发生的几个阶段。 迄今为止 关于阶段表达的遗传调节知之甚少 这些微生物或每个阶段的贡献中的特定基因 特定的表面蛋白使生物体的生物学使 Brucei锥虫的VSG基因除外。 神经氨酸酶是锥虫瘤的发育调节酶 克鲁兹和可能的毒力因子。 我们将结合 重组DNA技术，蛋白质生物化学和免疫学 表征酶并研究调节机制的技术 管理基因的表达。 我们的实验将作为 未来研究的模型旨在确定重要的组件 该生物的细胞内生存。