IMMUNOCHEMISTRY OF STREPTOCOCCAL MEMBRANE PROTEINS

链球菌膜蛋白的免疫化学

• 批准号: 3129159
• 负责人: IVO J VAN DE RIJN
• 金额: $ 11.28万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-06-01 至 1986-11-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3129159
• 关键词: Streptococcus; enzyme linked immunosorbent assay; gel electrophoresis; genetic strain; glomerulonephritis; hybridomas; immunochemistry; membrane activity; membrane structure; microorganism culture; microorganism growth; microorganism immunology; monoclonal antibody; rheumatic fever

The association of acute rheumatic fever and acute glomerulonephritis with antecedent group A streptococcal infections has been well documented. Cross-reactions between membrane from different types and strains of streptococci and human heart, brain and kidney tissue have been shown. The main objective of this study is to continue an in depth analysis of the streptococal membrane with particular emphasis on the relation(s) of antigenic and chemical composition to pathogenesis. Initially we will determine the effect of various conditions of media and growth rate on the qualitative and quantitative appearance of components of the membrane. Since distinctions have been made between skin and throat strains as well as rheumatogenic and nephritogenic strains, the membranes from these groups will be compared by two dimensional electrophoresis and immunological analysis. In addition, the effect of selective pressure on the components of interest will be determined. Once the above studies are underway, monoclonal antibodies to specific antigens will be produced using hybridoma technology. Furthermore, the patients's humoral immune response to the membranes components will also be analyzed. Finally, the cross-reactive antigens, the hyaluronic acid biosynthesis complex, the lipoteichoic acid deacylase, and the components unique to each group of strains are to be studied and charaterized. This study will also generate data on the basic structure and function of the streptococcal membrane. Identification of antigenic and enzymatic components of the membrane has received little attention in other laboratories. This study should yield important new information on the similarities and/or dissimilarities between this pathogen and non-pathogens whose membranes have received closer scrutiny. In the United States, rheumatic heart disease is reported to affect about 100,000 children and 1.7 million adults with over 13,000 deaths per year. In the third world nations, rheumatic fever is still a major public health problem. While there is no absolute figure on the incidence of acute poststreptococcal glomerulonephritis, endemic cases of skin nephritis still occur in many places throughout the world.

急性风湿热和急性肾小球肾炎与 前A组A链球菌感染已得到充分证明。 来自不同类型和应变的膜之间的交叉反应 已经显示了链球菌和人心，脑和肾脏组织。 这 这项研究的主要目的是继续深入分析 链球菌膜，特别强调 发病机理的抗原和化学组成。 最初我们会的 确定各种媒体条件和增长率对 膜成分的定性和定量外观。 由于皮肤和喉咙菌株也有区别 作为风湿性和肾病性菌株，来自这些组的膜 将通过二维电泳和免疫学比较 分析。 另外，选择性压力对组件的影响 将确定感兴趣的。 一旦上述研究正在进行， 使用杂交瘤产生对特定抗原的单克隆抗体 技术。 此外，患者对 膜组件也将进行分析。 最后，交叉反应 抗原，透明质酸的生物合成络合物，脂甲酸 脱酰基酶和每组菌株独有的成分应为 研究和充满特色。 这项研究还将生成有关基本的数据 链球菌膜的结构和功能。 识别 膜的抗原和酶成分几乎没有收到 其他实验室的注意。 这项研究应产生重要的新 有关此之间的相似性和/或差异的信息 病原体和膜的非公主受到了更仔细的审查。 在美国，据报道，风湿性心脏病会影响 每年有100,000名儿童和170万成年人，死亡人数超过13,000。 在第三世界国家，风湿热仍然是主要的公共卫生 问题。 虽然没有绝对的急性发生率 链球菌后肾小球肾炎，皮肤肾炎的流行病例仍然 发生在世界各地的许多地方。