ANALYSIS OF VARIANT STREPTOCOCCI FROM ENDOCARDITIS

心内膜炎变异链球菌的分析

• 批准号: 3341134
• 负责人: IVO J VAN DE RIJN
• 金额: $ 11.67万
• 依托单位: WAKE FOREST UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1982
• 资助国家: 美国
• 起止时间: 1982-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3341134
• 关键词: Streptococcus mitis; antibacterial antibody; bacterial antigens; bacterial endocarditis; bacterial polysaccharides; bacterial proteins; bactericidal immunity; carbohydrate sequence; cell adhesion; cell wall; chemical structure function; disease /disorder model; genetic strain; heart valves; host organism interaction; human tissue; laboratory mouse; laboratory rabbit; membrane activity; microorganism culture; microorganism immunology; monoclonal antibody; protein structure; serotyping; surface antigens; teichoate; virulence

Infective endocarditis is still an important disease that accounts for 0.3-3.0/1000 of all hospital admissions and the viridans streptococci remain the most frequent cause of subacute bacterial endocarditis. In this group of streptococci, the nutritionally variant streptococci account for 6-7% of the disease in man. To date, this is the first comprehensive analysis of the surface components of these strains. The overall objective of this research proposal is to continue a systematic study of the surface components of the nutritionally variant streptococci (NVS) in order to better understand the interaction of these bacteria with the heart valves in infective subacute bacterial endocarditis. Toward this objective we plan to determine the identity of the antigen(s) responsible for protection using the rabbit endocarditis model. The remaining surface components still to be analyzed in serotype I organisms for this function are the cell wall protein and ribitol teichoic acid. In addition we will characterize our present set of monoclonal antibodies against serotype I components. Produce further monoclonals against the serotype-specific antigens and the molecule(s) involved in adherence and/or colonization from serotype II and III organisms. Furthermore the identity of the antigen(s) responsible for protection using the rabbit endocarditis model for strains from serotypes II and III will be determined. Next, the NVS serotype II and III antigens if different from the respective protective antigens will be isolated and characterized. Finally, the analysis of antibody present in patients with NVS endocarditis will continue. The knowledge gained from the proposed studies will permit investigators to study the interaction between the surface of the NVS and the cardiac valves. In addition, it continues to lay the ground work for the rapid identification of these organisms upon isolation from blood culture. Finally, completion of these specific aims possibly could lead to a vaccine which would be effective in the population at high risk to this disease. Infective endocarditis is a serious disease in that if not created rapidly, secondary complications arise including renal and neurological damage. However 60% of the deaths caused by this disease is due to congestive heart failure. The NVS are important in that patients with endocarditis caused by these organisms have high rates of antimicrobial failure, relapse, and fatality.

感染性心内膜炎仍然是一种重要疾病 所有医院和Viridans的0.3-3.0/1000 链球菌仍然是亚急性最常见的原因 细菌性心内膜炎。 在这组链球菌中 营养变异链球菌占该疾病的6-7％ 在男人。 迄今为止，这是对 这些菌株的表面成分。 总体目标 研究建议是继续对表面进行系统的研究 营养变异链球菌（NVS）的成分 为了更好地了解这些细菌与 感染性亚急性细菌心内膜炎中的心脏瓣膜。 朝向这个目标，我们计划确定 使用兔心内膜炎负责保护的抗原 模型。 其余的表面成分仍待分析 该功能的血清型I生物是细胞壁蛋白和 核糖二甲酸。 此外，我们将描述我们现在 针对血清型I组分的一组单克隆抗体。 针对血清型特异性产生进一步的单克隆 抗原和涉及依从性和/或的分子 血清型II和III生物的定殖。 此外 使用该抗原的身份 血清型II和III菌株的兔心内膜炎模型将 可以确定。 接下来，NVS血清型II和III抗原 与各自的保护性抗原不同 并描述了。 最后，存在于 NVS心内膜炎患者将继续。 从拟议的研究中获得的知识将允许 研究人员研究表面之间的相互作用 NVS和心脏瓣膜。 此外，它继续铺设 以快速识别这些生物的地面工作 从血液培养中分离。 最后，完成这些特定的 目的可能导致疫苗有效 这种疾病高风险的人口。 感染性心内膜炎 是一种严重的疾病，因为如果不是迅速产生的次要 并发症发生在内，包括肾脏和神经系统损害。 然而，由于这种疾病造成的死亡中有60％是由于 充血性心力衰竭。 NV很重要，因为患者 这些生物引起的心内膜炎的发生率很高 抗菌衰竭，复发和死亡。