ANTIBIOTIC RESISTANCE OF BACTEROIDES

拟杆菌的抗生素耐药性

• 批准号: 3126657
• 负责人: Donald G. Guiney
• 金额: $ 13.61万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1980
• 资助国家: 美国
• 起止时间: 1980-02-01 至 1990-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3126657
• 关键词: Bacteroides; Enterobacteriaceae; Escherichia coli; R factors; antibiotics; antiinfective agents; bacterial DNA; bacterial genetics; gel electrophoresis; gene expression; genetic library; genetic regulation; host organism interaction; microorganism classification; microorganism interaction; microorganism metabolism; virulence

This project will provide a detailed understanding of the genetic mechanisms responsible for the spread of antibiotic resistance in Bacteroides. These anaerobic gram-negative bacteria are the predominant micro-organisms in the human gastro-intestianl tract, and function to maintain the normal microbial ecology of the bowel. In addition, Bacteroides are the most important isolates from anaerobic infections, and increasing antibiotic resistance in Bacteroides has complicated the treatment of these infections. Both plasmid and non-plasmid mediated transfer of antibiotic resistance has been described in Bacteroides, and indirect evidence for transposition of resistance markers has been reported. This project will utilize a novel gene transfer system recently developed to transfer plasmids from E. coli into Bacteroides. The system consists of a bifunctional shuttle vector capable of replication in both E. coli and Bacteroides, with transfer between E. coli and B. fragilis mediated by the broad host range plasmid RK2. This genetic system will be used to accomplish the following specific aims: 1) to locate and study the replication, transfer, and drug resistance genes on the Bacteroides R plasmid pCPl, 2) to characterize the mechanism of differential drug resistance gene expression in E. coli and Bacteroides, 3) to establish a gene library of Bacteroides DNA in E. coli, 4) to use the library to study the mechanism of the novel gene transfer systems that operate in Bacteroides in the absence of plasmids, and 5) to elucidate the mechanism of clindamycin resistance transposition in Bacteroides. This project will establish a genetic system in Bacteroides using current recombinant DNA technology. Such a system will facilitate the analysis not only of antibiotic resistance, but of physiology, metabolism, and virulence properties of these important anaerobes.

该项目将提供对遗传的详细理解 负责抗生素耐药性传播的机制 细菌。 这些厌氧革兰氏阴性细菌是主要的 人类胃肠道的微生物，并发挥作用 保持肠道正常的微生物生态学。 此外， 菌糖是来自厌氧感染的最重要的分离株，并且 菌列中抗生素耐药性的增加已使其复杂化 治疗这些感染。 质粒和非质粒介导 在细菌下已经描述了抗生素耐药性的转移，并且 抗电阻标记的间接证据已经是 报告。 该项目最近将利用一个新型的基因转移系统 开发是将质粒从大肠杆菌转移到菌孢子中。 系统 由双方班车向量组成，能够在两个E中复制。 大肠杆菌和细菌，在大肠杆菌和脆弱的大肠杆菌之间转移 由广泛的宿主范围质粒RK2介导。 这个遗传系统将是 用于完成以下特定目的：1）定位和研究 菌孢子上的复制，转移和耐药性基因 质粒PCPL，2）表征差异药物的机制 大肠杆菌和细菌的抗性基因表达，3）建立 大肠杆菌中菌烯型DNA的基因库，4）使用该文库研究 新型基因转移系统的机制 在没有质粒的情况下菌孢子，5）阐明了机制 菌霉素的抗性抗性转座。 这个项目将 使用电流重组DNA在细菌下建立遗传系统 技术。 这样的系统不仅会促进 抗生素耐药性，但生理，代谢和毒力 这些重要厌氧的特性。