Investigating the role of a kinesin gene in butterfly mimicry

研究驱动蛋白基因在蝴蝶拟态中的作用

基本信息

批准号：
BB/H01439X/1
负责人：
Chris Jiggins
金额：
$ 57.04万
依托单位：
University of Cambridge
依托单位国家：
英国
项目类别：
Research Grant
财政年份：
2011
资助国家：
英国
起止时间：
2011 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=BB%2FH01439X%2F1
关键词：
Investigating role kinesin gene butterfly

项目摘要

Mimicry among butterfly species is a classic example of evolution and adaptation. The brightly coloured neotropical Heliconius butterflies are one of the best studied examples, but the molecular genetic basis of mimicry remains poorly understood. In particular mimicry offers an opportunity to study the repeatability of evolution, as the same patterns emerge again and again in divergent lineages. The project falls into two broad areas, first we use cutting edge sequencing technology to make a genetic map of the H. melpomene genome. This will be used to help assemble the genome sequence currently being generated. This will form the basis for a genome-wide survey of adaptive divergence between H. melpomene races. Divergent geographic populations of this species form narrow hybrid zones where they hybridise and exchange genes. Thus, narrow regions of the genome controlling wing patterns are genetically differentiated against a background of extensive recombination. This offers a powerful opportunity to identify changes responsible for wing pattern differentiation. We will first characterise variation within and between races by genome resequencing at low coverage. Then we will use novel 'sequence capture' technology to enrich genomic DNA for regions of interest from 96 individuals, taken from six phenotypic races of H. melpomene. The experiment will be designed to sample two chromosomal regions containing wing patterning genes, and a further 12,000 variable sites located across the genome. This will offer a unique genome-wide analysis of parallel divergence between the six populations sampled. In particular we aim to determine a) how much of the genome is involved in colour pattern divergence b) whether the same regions are implicated across independent hybrid zones c) estimate the age of the alleles involved in wing pattern divergence and d) identify putative functional sites for further analysis. The second major aim of the project is to investigate the kinesin gene that represents a strong candidate locus for controlling the red forewing band of H. melpomene. We will study the spatial distribution of kinesin gene expression patterns between divergent phenotypes, in order to test whether spatial regulation underlies pattern regulation. Many genes show different variants generated by alternative splicing, generating variant forms of the protein containing alternative forms of the exons. Alternative splicing is a potentially powerful but under-explored mechanism that could generate evolutionarily relevant variation. We have evidence for alternative splicing the Kinesin gene, and here will characterise the isoforms of this gene and test for correlations between isoform expression and wing phenotype. We will investigate the molecular function of the gene, including a search for other molecules that interact with the kinesin protein, and test to confirm its motor function. Finally, we will develop trangenics methods for explicitly testing the function of the kinesin gene in wing pattern specification in divergent races of H. melpomene. The major gene dominant control of the red band means that we expect to be able to generate a red-banded phenotype by expressing the 'red' kinesin allele in a yellow banded phenotype. This will provide the first explicit test of function for a gene causing pattern variation in any butterfly.

蝴蝶物种中的模仿是进化和适应的经典例子。鲜艳的新热带Heliconius蝴蝶是研究最佳的例子之一，但模仿的分子遗传基础仍然鲜为人知。特别是模仿提供了研究进化的重复性的机会，因为相同的模式在不同的谱系中一次又一次出现。该项目属于两个广泛的领域，首先我们使用尖端测序技术来制作梅尔托烯基因组的遗传图。这将用于帮助组装当前正在生成的基因组序列。这将构成全基因组对梅尔帕烯种族之间适应性差异的调查的基础。该物种的不同地理种群形成狭窄的杂种区，它们在其中杂交并交换基因。因此，基因组控制机翼模式的狭窄区域在广泛的重组背景下在遗传上有分化。这提供了一个有力的机会来确定负责机翼模式差异的变化。我们将首先通过在低覆盖范围内重新陈述种族组内和种族之间的变化。然后，我们将使用新颖的“序列捕获”技术来富含96个个体感兴趣区域的基因组DNA，从六个表型种族中获得。该实验将旨在采样两个含有机翼模式基因的染色体区域，并在整个基因组上进行另外12,000个可变位点。这将提供独特的全基因组分析，对所采样的六个种群之间的平行差异分析。特别是我们的目的是确定a）颜色模式差异涉及多少基因组涉及b）在独立的杂种区域中是否与相同的区域有关c）估计涉及机翼模式差异的等位基因的年龄和d）识别推定的功能位点以进行进一步分析。该项目的第二个主要目的是调查代表强大候选基因座的动力蛋白基因，用于控制梅尔托烯的红色前提带。我们将研究不同表型之间驱动蛋白基因表达模式的空间分布，以测试空间调节是否构成了模式调节的基础。许多基因显示出由替代剪接产生的不同变体，从而产生含有外显子替代形式的蛋白质的变体形式。替代剪接是一种潜在强大但探索不足的机制，可以产生进化相关的变化。我们有替代剪接驱动蛋白基因的证据，此处将表征该基因的同工型，并测试同工型表达和机翼表型之间的相关性。我们将研究基因的分子功能，包括搜索与驱动蛋白蛋白相互作用的其他分子，并测试以确认其运动功能。最后，我们将开发用于明确测试旋蛋白基因在旋翼种族中的机翼模式规范中的功能的跨齿方法。红色条带的主要基因主导控制意味着我们希望能够通过在黄色带状表型中表达“红色”驱动等位基因来产生红带表型。这将为引起任何蝴蝶的模式变化的基因的功能进行首次显式测试。

项目成果

期刊论文数量（10）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Speciation in Heliconius Butterflies: Minimal Contact Followed by Millions of Generations of Hybridisation

袖蝶的物种形成：最小的接触，随后是数百万代的杂交

DOI：
10.1101/015800
发表时间：
2015
期刊：
影响因子：
0
作者：
Martin S
通讯作者：
Martin S

Genome-wide patterns of divergence and gene flow across a butterfly radiation

蝴蝶辐射中的全基因组分歧和基因流模式

DOI：
10.1111/j.1365-294x.2012.05730.x
发表时间：
2012
期刊：
Molecular Ecology
影响因子：
4.9
作者：
Nadeau N
通讯作者：
Nadeau N

The diversification of Heliconius butterflies: what have we learned in 150 years?

DOI：
10.1111/jeb.12672
发表时间：
2015-08-01
期刊：
JOURNAL OF EVOLUTIONARY BIOLOGY
影响因子：
2.1
作者：
Merrill, R. M.;Dasmahapatra, K. K.;Yu, Q.
通讯作者：
Yu, Q.

Multilocus species trees show the recent adaptive radiation of the mimetic heliconius butterflies.

DOI：
10.1093/sysbio/syv007
发表时间：
2015-05
期刊：
Systematic biology
影响因子：
6.5
作者：
Kozak KM;Wahlberg N;Neild AF;Dasmahapatra KK;Mallet J;Jiggins CD
通讯作者：
Jiggins CD

Estimation of the spontaneous mutation rate in Heliconius melpomene.

DOI：
10.1093/molbev/msu302
发表时间：
2015-01
期刊：
Molecular biology and evolution
影响因子：
10.7
作者：
Keightley PD;Pinharanda A;Ness RW;Simpson F;Dasmahapatra KK;Mallet J;Davey JW;Jiggins CD
通讯作者：
Jiggins CD