NEUROENDOCRINOLOGY OF REPRODUCTIVE AGING

生殖衰老的神经内分泌学

• 批准号: 3116754
• 负责人: S KALEEM QUADRI
• 金额: $ 6.59万
• 依托单位: KANSAS STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1987
• 资助国家: 美国
• 起止时间: 1987-08-01 至 1991-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3116754
• 关键词: aging; aromatic L aminoacid decarboxylase; catecholamine inhibitor; dihydroxyphenylalanine; dopamine; dopamine beta monooxygenase; epinephrine; estrus; gonadotropin releasing factor; high performance liquid chromatography; hormone metabolism; hormone regulation /control mechanism; hypothalamus; laboratory rat; neuroendocrine system; neurotransmitter metabolism; norepinephrine; perfusion; progesterone; prolactin; pseudopregnancy; serotonin; tyrosine 3 monooxygenase

This application's long-term objectives are investigation of neuroendocrine changes responsible for age-related loss of reproductive cycles. The specific aims of this application are to use the technique of high performance liquid chromatography to determine age-related changes in the activities of enzymes involved in the synthesis and degradation of catecholamines and serotonin in discrete areas of the hypothalamus. Age-related changes in the contents and release of catecholamines and serotonin and gonadotropin releasing hormone from discrete areas of the hypothalamus will also be determined. The technique of push-pull perfusion will be used to determine the release of neurotransmitters and gonadotropin releasing hormone from hypothalamic areas. The data on the activities of the enzymes and on the content and release of neurotransmitters will be collected on a long-term basis during various patterns of acyclicity and correlated with serum levels of luteinizing hormone, prolactin, estradiol and progesterone and with vaginal cytology. Changes in these parameters will also be determined after acyclic animals have been treated with -dopa and after normal cyclic animals have been on chronic treatment with estradiol. The data collected in this project will explain in detail the age- related changes in the activities of the enzymes involved in the synthesis and degradation of catecholamines and serotonin and in the hypothalamic content and release of these neurotransmitters. These data will be new and will add to our understanding of the primary causes of loss of reproductive cyclicity in aging.

该应用程序的长期目标是调查 神经内分泌变化导致与年龄相关的损失 生殖周期。 本应用的具体目的是使用 高性能液相色谱法确定与年龄相关的 涉及合成的酶的活动的变化和 在离散区域中儿茶酚胺和5-羟色胺的降解 下丘脑。 与年龄相关的内容变化和 释放儿茶酚胺，5-羟色胺和促性腺激素 从下丘脑离散区域释放激素将 也可以确定。 推扣灌注技术将是 用于确定神经递质的释放和 促性腺激素从下丘脑区域释放激素。 这 有关酶的活动以及内容的数据 将长期收集神经递质的释放 在各种环性模式中的基础，并与 黄体生成激素，催乳素，雌二醇和 孕酮和阴道细胞学。 这些变化 在无环动物具有后，也将确定参数 用-dopa治疗，在正常的循环动物具有 接受雌二醇的慢性治疗。 该项目中收集的数据将详细解释年龄 - 涉及的酶的活动的相关变化 儿茶酚胺和5-羟色胺的合成和降解以及 这些神经递质的下丘脑含量和释放。 这些数据将是新的，将增加我们对 衰老中生殖循环性丧失的主要原因。