SYNAPTIC MECHANISMS OF ETHANOL ACTION IN THE CEREBELLUM

小脑中乙醇作用的突触机制

• 批准号: 3111462
• 负责人: CHIMING HUANG
• 金额: $ 12.75万
• 依托单位: UNIVERSITY OF MISSOURI KANSAS CITY
• 依托单位国家: 美国
• 财政年份: 1988
• 资助国家: 美国
• 起止时间: 1988-04-01 至 1991-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3111462
• 关键词: alcoholic beverage consumption; cats; cerebellar Purkinje cell; cerebellum; dosage; drug abuse; drug tolerance; electrophysiology; ethanol; evoked potentials; histology; methanol poisoning; microelectrodes; neural transmission; neuroanatomy; neurons; stereotaxic techniques; synapses

The proposed studies will examined the temporal discharge properties of single cerebellar neurons at low (0-1 gm/kg), medium (1-2 gm/kg), and high (3-4 gm/kg) doses of ethanol. This information, together with the neuron's sensitivity toward ethanol, will be utilized to evaluate the role of reverberation from recurrent feedback loops in the formation of patches of ethanol-sensitive and ethanol-resistant cells. Anatomical substrates underlying the differential sensitivity shall be investigated by correlating the topographical distribution of cells displaying characteristic temporal discharge patterns with the topographic distribution of feedback collaterals from Purkinje cells. An important additional objective is to determine the correlation between cellular sensitivity to ethanol with the development of tolerance. Dose-response relationships will be monitored to document the onset as well as the kinetics of development of tolerance for single neurons in the cerebellum. These studies shall be carried out using the cat as a model. The overall objective is to determine the synaptic mechanisms involved in the loss of cerebellar function and the development of tolerance as a result of ethanol. This knowledge should be important to the understanding of intoxication, tolerance, and dependence due to alcohol abuse.

拟议的研究将检查时间放电 低（0-1 gm/kg）的单小脑神经元的特性， 培养基（1-2 gm/kg）和高（3-4 gm/kg）乙醇的高剂量。 这 信息，以及神经元对 乙醇将用于评估混响的作用 从复发反馈回路形成 乙醇敏感和耐乙醇的细胞。 解剖学 差异灵敏度的基础应为 通过将细胞的地形分布相关联 显示特征的时间放电模式 来自浦肯野的反馈抵押品的地形分布 细胞。 一个重要的另一个目标是确定 细胞对乙醇的敏感性与乙醇之间的相关性 宽容的发展。 剂量反应关系将是 受到监测以记录​​发作和动力学 小脑中单神经元的耐受性的发展。 这些研究应使用猫作为模型进行。 这 总体目标是确定突触机制 参与小脑功能的丧失和发展 乙醇的耐受性。 这些知识应该是 对理解中毒，宽容和 由于酗酒而导致的依赖。