GENETICS OF VIRULENCE DETERMINANTS OF BLACK-PIGMENTED BACTEROIDES

黑色拟杆菌毒力决定因素的遗传学

• 批准号: 3854360
• 负责人: DAVID DYER
• 金额: --
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3854360
• 关键词: Bacteroides gingivalis; Escherichia coli; bacterial pigment; cell adhesion; chemical structure function; endonuclease; germ free condition; laboratory mouse; laboratory rat; membrane activity; microorganism culture; microorganism genetics; oral bacteria; periodontium disorder; pilus; plasmids; restriction fragment length polymorphism; saliva; transposon /insertion element

Black-pigmented members of the bacterial genus Bacteroides (BPB's) are colonists of the oral cavity, and are frequently found in association with oro-facial infections. Colonization of the oral cavity by these organisms must involve their adhesion to some component of the environment in order to resist being flushed away by saliva or the flow of crevicular fluid. Almost all strains of BPB's examined to date carry fimbriae: polymers of a protein subunit (fimbrilin) which form filaments that project from the cell surface. Considerable evidence has been accumulated from other bacterial species to show that fimbriae mediate specific adherence to a variety of surfaces such as epithelial cells or other bacteria. We proposed to test this hypothesis by determining the adhesive properties of BPB fimbriae. Since the BPB's form a group of related species, the question arises as to the evolutionary relationship of their fimbriae. Thus, we wish to determine if the BPB fimbriae are ancestrally related. If so, do they perform a conserved function or different functions? If they are unrelated but perform similar functions, is this due to convergent evolution? Relatedness will be determined by comparison of the nucleotide sequences of the cloned genes encoding the fimbrial protein subunits. Completion of these studies will provide, at the molecular level, an understanding of the strategies used by BPB's for fimbrial mediated adhesions. Information will be gained on the evolutionary pathways leading to these strategies and their potential roles in colonization of the oral cavity.

细菌属细菌的黑色色素成员 （BPB）是口腔的殖民者，经常被发现 与原始感染有关。 口腔殖民 这些生物的腔必须涉及对某些生物的粘附 环境的组成部分以抗拒被冲洗 通过唾液或刺流的流动。 几乎所有的菌株 BPB迄今已检查的携带纤维膜：蛋白质的聚合物 亚基（Fimbrilin），形成从细胞发射的细丝 表面。 来自其他证据已有大量证据 细菌物种表明fimbriae介导了特异性 遵守各种表面，例如上皮细胞或其他 细菌。 我们建议通过确定 BPB Fimbriae的粘附特性。 由于BPB的形式A 相关物种群，关于进化的问题 他们的fimbriae的关系。 因此，我们希望确定是否 BPB Fimbriae与祖先相关。 如果是这样，他们是否执行 保守的功能还是不同的功能？ 如果他们无关 但是执行类似的功能，这是否是由于收敛性进化而引起的？ 相关性将通过核苷酸的比较来确定 编码纤维蛋白的克隆基因的序列 亚基。 这些研究的完成将提供 分子水平，对BPB使用的策略的理解 用于纤维化介导的粘附。 信息将获得 导致这些策略及其的进化途径 在口腔定植中的潜在作用。