Studies of E. coli Min proteins

大肠杆菌 Min 蛋白的研究

基本信息

批准号：
6780584
负责人：
LAWRENCE I ROTHFIELD
金额：
$ 36.25万
依托单位：
UNIVERSITY OF CONNECTICUT SCH OF MED/DNT
依托单位国家：
美国
项目类别：
财政年份：
2000
资助国家：
美国
起止时间：
2000-02-01 至 2009-03-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/6780584
关键词：
Escherichia coli bacterial genetics bacterial pigment bacterial proteins biochemistry cell cycle chromosome movement cytoskeleton fluorescence microscopy gene mutation protein localization protein protein interaction protein structure protein structure function

项目摘要

DESCRIPTION (provided by applicant): The long-range goal of this project is to determine the mechanism used by bacteria to select the proper cell division site at midcell. The three Min proteins, MinC, MinD and MinE, are required for proper site selection. The proteins have a unique cellular localization pattern and undergo a unique pole-to-pole oscillatory cycle. We have recently shown that the MinCDE proteins are organized into spiral filaments that wind around the cell between the two poles in a cytoskeletal-like structure that appears distinct from a coiled structure formed by the MreB protein. During the proposed grant period we will use a combination of fluorescence microscopy, biochemistry and genetics to achieve the following aims: i. Min protein structure and function-- To determine the effects of mutations that interfere with the topological specificity function of the MinCDE system, ii. Long-range organization of the Min system-- To define the organization of the MinDE cytoskeletal-like elements within the cell, to determine whether the Min and MreB spiral filaments are separate structures, to characterize proteins that interact with MinD and MinE, and to determine whether there is a relationship between the Min spiral filaments and chromosome segregation, iii. MreB-- To isolate the MreB and Min cytoskeletal-like structures, to characterize proteins that interact with MreB, and to define the behavior of the MreB cytoskeletal-like structures during the course of the cell cycle.

描述（由申请人提供）：该项目的远程目标是确定细菌在Midcell选择适当的细胞分裂位点的机制。正确选择现场需要三分钟蛋白，即Minc，Mind和Mine。蛋白质具有独特的细胞定位模式，并经历了独特的杆到极振荡周期。我们最近表明，MinCDE蛋白被组织成螺旋丝中，在细胞骨骼样结构中，两极之间的细胞周围绕着细胞缠绕，这些结构似乎与MREB蛋白形成的盘绕结构不同。在拟议的赠款期间，我们将使用荧光显微镜，生物化学和遗传学的组合来实现以下目的：i。最小蛋白质结构和功能 - 确定干扰Mincde系统拓扑特异性功能的突变的影响，ii。最小系统的远程组织 - 定义细胞内的Minde细胞骨架状元素的组织，以确定最小值和MREB螺旋丝是否是单独的结构，以表征与思想和我的互动的蛋白质，并确定是否在最小螺旋细胞和染色体播种的蛋白质之间存在关系。 MREB- - 分离MREB和最小细胞骨架样结构，以表征与MREB相互作用的蛋白质，并在细胞周期过程中定义MREB细胞骨架样结构的行为。