CENTRAL VIRUS CORE

中央病毒核心

• 批准号: 3818967
• 负责人: GEORGE M SHAW
• 金额: --
• 依托单位: UNIVERSITY OF ALABAMA AT BIRMINGHAM
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/3818967
• 关键词: AIDS; biohazard control; biomedical facility; biomedical resource; genetic manipulation; human immunodeficiency virus; immunological substance; microorganism culture; monoclonal antibody; transposon /insertion element

The Central Virus Core (CVC) will play a pivotal role in the Birmingham CFAR. The CVC will provide a centralized, fully- equipped, and highly supervised Biosafety Level 3 (BSL 3) laboratory facility for all infectious HIV work by center members and will further serve as a Center resource for the maintenance and storage of thoroughly characterized stocks of HIV virus isolates, infected cell lines, and virus-specific monoclonal and polyclonal antibodies. Although all recombinant HIV plasmid and phage constructs will be catalogued, propagated, and provided to CFAR members through the Molecular Biology Core, the CVC will play an important role in the initial construction of these recombinant HIV containing vectors which are often derived initially from primary or passaged cultures of HIV virus. The CVC directors (Drs. Shaw and Hahn) have demonstrated the successful application of the concept of a CVC for provision of virus-specific reagents by their current ongoing contract with the National Institutes of Allergy and Infectious Diseases for the provision of HIV proviral clones, growing stocks, and relevant genetic information for the NIAID AIDS Reagent Program (Appendix). The six programmatic areas comprising the CFAR Basic Sciences Program and the three programmatic areas comprising the Clinical Sciences Program will maximally utilize the CVC as the primary site for all infectious HIV work, as a source for HIV related reagents, and as a resource for information and experimental design and development relevant to their programmatic areas. Thirty-three NIH funded CFAR members have been identified who will utilize the CVC services. The CVC facility will be centrally located to these investigators and will consist of a 2,000 sq.ft. containment facility having six individual culture rooms, a common shared equipment area containing centrifuges, automated cell counter, microscopes, freezers, and all other equipment required for virus culture and analysis. The CVC will be operated on an at-cost reimbursement basis from the research funds of its users. The CVC will limit potential exposure to infectious HIV to a single tightly controlled laboratory facility providing maximal safety and efficiency, obviate duplication of equipment, and make available to all CFAR members full facilities for HIV research and a complete spectrum of fully characterized, high quality HIV related reagents.

中央病毒核心（CVC）将在 伯明翰CFAR。 CVC将提供集中式的，充分的 配备和高度监督的生物安全级别3（BSL 3） 中心成员的所有感染HIV工作的实验室设施 并将进一步作为维护和维护的中心资源 储存彻底特征的HIV病毒分离株的库存， 感染的细胞系，病毒特异性单克隆和多克隆 抗体。 尽管所有重组HIV质粒和噬菌体 构造将被分类，传播并提供给CFAR 通过分子生物学核心成员，CVC将发挥 在这些重组HIV的初始结构中的重要作用 包含通常最初源自主要的向量 或艾滋病毒传递的培养物。 CVC导演（肖博士 和Hahn）证明了成功的应用 CVC用于提供病毒特异性试剂的概念 当前与美国国家过敏研究所的持续合同 以及为提供HIV病毒的克隆提供的传染病， 种植的库存和NIAID AIDS的相关遗传信息 试剂程序（附录）。 六个编程区域包括 CFAR基本科学计划和三个编程领域 包括临床科学计划将最大程度地利用 CVC作为所有感染HIV工作的主要网站，作为来源 对于艾滋病毒相关的试剂，作为信息的资源和 实验设计和开发与他们的程序化有关 区域。 已经确定了33个NIH资助的CFAR成员 谁将利用CVC服务。 CVC设施将是 位于这些调查人员的集中，将由 2,000平方英尺 遏制设施有六个个人文化 房间，一个共享设备区域，包含离心机， 自动细胞计数器，显微镜，冰柜和所有其他 病毒培养和分析所需的设备。 CVC会 从研究中以成本的报销基础进行操作 用户的资金。 CVC将限制潜在的接触 传染性艾滋病毒对一个紧密控制的实验室设施 提供最大的安全性和效率，消除了 设备，并向所有CFAR成员提供完整的设施 用于HIV研究和完全表征的完整范围 高质量艾滋病毒相关的试剂。