PRECISION UNIVERSAL COMPENSATOR FOR IMAGING POLARIMETRY

用于成像旋光测量的精密通用补偿器

• 批准号: 2459591
• 负责人: CLIFFORD C HOYT
• 金额: $ 19.48万
• 依托单位: CAMBRIDGE RESEARCH AND INSTRUMENTATION
• 依托单位国家: 美国
• 财政年份: 1994
• 资助国家: 美国
• 起止时间: 1994-07-01 至 1999-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2459591
• 关键词: biomedical equipment development; birefringences; computer program /software; digital imaging; light microscopy; liquid crystal; optical polarization; polarimetry

Researchers at the Marine Biological Laboratory, Woods Hole, MA, are developing a polarized light microscope (pol-scope) with substantial advantages over traditional pol-scopes in speed, resolution, and versatility. The microscope, with a new liquid crystal (LC) universal compensator, produces images in under one second that measure optical anisotropies irrespective of sample orientation and at the spatial resolution of the microscope. While traditional pol-scopes are typically used to measure optical anisotropy in a single specimen point, the new microscope provides measurements over a large area with high spatial and temporal resolution. We propose to build on successful Phase I results to complete the development of an enhanced LC universal compensator with software that implements MBL's algorithm for calculating retardance magnitude and orientation. These developments will make available to the scientific community the first capability to visualize and analyze quantitatively the dynamic organization of macromolecular assemblies, independent of their orientation, in living cells. Examples of such assemblies include the mitotic spindle, the contractile ring during cell cleavage or the microtubule organization during cell interphase. We anticipate that an attractively priced accessory based on this research will find solid niche markets for scientific investigations, as well as clinical and industrial applications. PROPOSED COMMERCIAL APPLICATION: This research will lead to a universal compensator with significant advantages over conventional devices, at a comparable price. Applications in microscopic and macroscopic imaging range from cell biological studies, such as the dynamics of the cytoskeleton, stack membranes, and DNA tertiary structures, to clinical routing investigations and industrial quality control.

马萨诸塞州伍兹霍尔海洋生物实验室的研究人员 开发偏光显微镜（pol-scope） 与传统偏振镜相比，在速度、分辨率和 多功能性。 配备新型通用液晶 (LC) 的显微镜 补偿器，在一秒内生成测量光学的图像 各向异性与样品方向和空间无关 显微镜的分辨率。虽然传统的偏振镜通常是 用于测量单个样本点的光学各向异性，新 显微镜提供大面积的测量，具有高空间和 时间分辨率。 我们建议在第一阶段成功结果的基础上完成 开发增强型 LC 通用补偿器，其软件 实现 MBL 的算法来计算延迟幅度和 方向。 这些进展将为科学界提供便利 社区第一个可视化和定量分析的能力 大分子组装体的动态组织，独立于 它们在活细胞中的方向。 此类组件的示例包括 有丝分裂纺锤体，细胞分裂期间的收缩环或 细胞间期的微管组织。 我们预计， 基于这项研究的价格有吸引力的配件将会找到可靠的 科学研究以及临床和临床的利基市场 工业应用。 拟议的商业应用：这项研究将导致普遍的 补偿器与传统装置相比具有显着优势， 可比价格。 在显微和宏观成像中的应用 范围从细胞生物学研究，例如细胞动力学 细胞骨架、叠层膜和 DNA 三级结构，以应用于临床 路线调查和工业质量控制。