MINORITY SCHOOL FACULTY DEVELOPMENT AWARD

少数族裔学校教师发展奖

• 批准号: 3088276
• 负责人: DIANE A BLAKE
• 金额: $ 6.77万
• 依托单位: MEHARRY MEDICAL COLLEGE
• 依托单位国家: 美国
• 财政年份: 1985
• 资助国家: 美国
• 起止时间: 1985-09-30 至 1990-09-29
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3088276
• 关键词: basement membrane; cell adhesion; cell cell interaction; cell growth regulation; extracellular matrix; gel filtration chromatography; heparan sulfate; membrane reconstitution /synthesis; membrane structure; proteoglycan; radiotracer; tissue /cell culture; vascular endothelium

The basement membrane synthesized by microvascular endothelial cells is thought to play an important role in the proper physiological functioning of this cell type. Preliminary evidence obtained in vitro from a number of laboratories has indicated that microvascular endothelial cells and their underlying basement membrane have an interactive relationship; changes in the molecular composition of the growth substratum induce changes in the growth, morphology, and biosynthetic capacity of the overlying cell layer. At present, however, there are no systematic studies available which detail how specific changes in the basement membrane may "feedback" and influence the associated cell layer. It is the ultimate goal of this research to determine the biological function of complex glycoconjugates in this interactive relationship. The methodology described in this proposal provides for systematic and controlled modification of the glycoconjugate structures present in basement membrane. Microvascular endothelial cells will subsequently be plated onto control or modified matrices and their capacity to synthesize three specific basement membrane components, collagen, laminin, and heparan sulfate proteoglycan, will be assessed. Any changes in the biosynthetic capacity of cells cultured on control versus modified matrices may then be attributed directly to the structural changes in the proteoglycan of glycoprotein components of extracellular matrix. it is hoped that the systematic modification experiments described in this proposal will be useful in identifying those portions of the complex glycoconjugate structure that are recognized as biologically significant by the overlying cell layer.

微血管内皮细胞合成的基底膜是 被认为在正常的生理功能中发挥重要作用 这种细胞类型。 从许多体外实验中获得的初步证据 实验室表明微血管内皮细胞及其 下层基底膜之间存在相互作用关系；的变化 生长基质的分子组成引起变化 上覆细胞层的生长、形态和生物合成能力。 但目前尚无系统研究详细阐述 基底膜的具体变化如何“反馈”并影响 相关的细胞层。 这项研究的最终目标是 确定复杂糖复合物的生物学功能 互动关系。 本提案中描述的方法 提供糖复合物的系统和受控修饰 存在于基底膜中的结构。 微血管内皮细胞 随后将被镀到对照或修改的基质上及其 合成三种特定基底膜成分的能力， 将评估胶原蛋白、层粘连蛋白和硫酸乙酰肝素蛋白多糖。 任何 对照培养细胞与对照培养细胞生物合成能力的变化 修改后的矩阵可以直接归因于结构变化 存在于细胞外基质糖蛋白成分的蛋白多糖中。 它 希望本文中描述的系统修改实验 提案将有助于确定综合体的这些部分 被认为具有生物学意义的复合糖结构 上覆的细胞层。