REPLICATION OF RUBELLA VIRUS

风疹病毒的复制

• 批准号: 3070964
• 负责人: TERYL K FREY
• 金额: $ 6.86万
• 依托单位: GEORGIA STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-09-01 至 1994-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3070964
• 关键词: Rubivirus; cellular immunity; gel electrophoresis; gene expression; genetic manipulation; genetic translation; immunofluorescence technique; in situ hybridization; laboratory rabbit; molecular cloning; nucleic acid probes; nucleic acid sequence; rubellas; site directed mutagenesis; tissue /cell culture; transposon /insertion element; virus genetics; virus infection mechanism; virus protein; virus replication

The applicant, Teryl K. Frey, has been at his position as an Assistant Professor in the Biology Department at Georgia State University for almost six years. In that time, he has been able to develop in his laboratory a research program on the molecular biology of rubella virus. Rubella virus is a major human pathogen. Although controlled in the United States by a vaccination program, the virus is endemic in the rest of the world. Complication arising from rubella virus infection are birth defects and persistent infection. The vaccine strain virus has been shown to have the capacity of persisting. Persistent rubella virus infection has been associated with vaccine failure, some forms of chronic arthritis, and a rare, fatal neurodegenerative disorder. The molecular biology of rubella virus has only recently been elucidated in any detail. A major aim of the proposed research is to continue study of the molecular biology of this virus. A professional goal of the applicant is to apply the information elucidated from the study of the molecular biology of viruses to gaining understanding of viral pathogenesis in humans, particularly with regards to persistent infection, and to resolving some of the health problems caused by viruses. Therefore, the second major aim of the proposed research is to initiate studies directed toward the goal of approaching human health problems associated with rubella virus with molecular biological methods. An RCDA award is being applied for to allow the applicant the extra time needed to pursue this latter research aim which represents an amplification of the applicant's current research scope. The specific experimental initiatives to be undertaken in this effort are, first, to introduce the structural proteins of rubella virus into expression vectors with which candidate vaccines or diagnostic reagents can be designed. The second is to develop an assay with which to distinguish the wild type and vaccine viruses to determine if certain complications such as birth defects and chronic disease due to persistent rubella virus infection are caused by wild type infection, vaccination, or both. Thirdly, background studies will be done which eventually will lead to a study of rubella virus persistence in human. This involves developing vectors with which to study the cell mediated immune response to rubella virus and designing probes for the detection of persistent rubella virus infection in humans.

申请人Teryl K. Frey一直担任助手 佐治亚州立大学生物学系教授 将近六年。 那时，他能够在他的 实验室关于风疹病毒分子生物学的研究计划。 风疹病毒是主要的人类病原体。 虽然在 美国通过疫苗接种计划，该病毒在其余 世界。 风疹病毒感染引起的并发症是 出生缺陷和持续感染。 疫苗菌株病毒具有 被证明具有持久能力。 持续的风疹病毒 感染与疫苗衰竭有关，某些形式 慢性关节炎和一种罕见的致命神经退行性疾病。 这 风疹病毒的分子生物学直到最近才阐明 任何细节。 拟议研究的主要目的是继续研究 该病毒的分子生物学。 一个专业目标 申请人将应用从研究的研究中阐明的信息 病毒的分子生物学，以了解病毒 人类的发病机理，特别是关于持续的 感染，并解决由 病毒。 因此，拟议研究的第二个主要目的是 启动针对接近人类健康的目标的研究 与分子生物学的风疹病毒相关的问题 方法。 正在申请RCDA奖励，以允许申请人 追求后一个研究目标所需的额外时间，该目标代表 申请人当前的研究范围的放大。 具体 在这项工作中要进行的实验计划首先是 将风疹病毒的结构蛋白引入表达 可以使用候选疫苗或诊断试剂的向量是 设计。 第二个是开发一个区分的测定法 野生型和疫苗病毒以确定某些并发症是否 例如由于风疹持续存在的出生缺陷和慢性疾病 病毒感染是由野生型感染，疫苗接种或两者引起的。 第三，将进行背景研究，最终将导致 对人的风疹病毒持久性研究。 这涉及发展 研究细胞介导的对风疹的免疫反应的向量 病毒和设计用于检测持续风疹病毒的探针 人类感染。