REACTION CENTER DYNAMICS

反应中心动力学

• 批准号: 3072909
• 负责人: CRAIG C SCHENCK
• 金额: $ 6.44万
• 依托单位: COLORADO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1989
• 资助国家: 美国
• 起止时间: 1989-07-01 至 1994-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/3072909
• 关键词: bacterial proteins; conformation; crosslink; electron transport; fluorescence spectrometry; genetic manipulation; hydrogen bond; infrared spectrometry; molecular dynamics; nucleic acid sequence; oxidation reduction reaction; photosynthesis; photosynthetic bacteria; protein structure; protein structure function; site directed mutagenesis; tautomer; thermodynamics

This proposal requests a Research Career Development Award for C. Schenck. The long-term research objective of the candidate is to understand the relationship between structure and function of membrane proteins. Other long term career objectives include participation in the establishment of a stimulating training environment for graduate and post-doctoral study in biochemistry and biophysics at Colorado State University. A multidisciplinary approach to problem solving will be encouraged. A first step toward these goals is the development of a vigorous independent research program. An RCDA would allow the candidate to focus complete attention on research and research-related activities for a five year period. Specifically, an RCDA would: 1) Decrease non- research related teaching and administrative activities; 2) Allow the candidate to develop expertise in two research areas closely related to his own research, but requiring further training; 3) Benefit the Department by encouraging collaborative research interactions with at least two other faculty members in a growing research-oriented department. The bacterial reaction center is an interesting system for testing theories of biological electron transfer and protein dynamic behavior. The Research Plan describes the Candidate's short-term research goals for assessing the relationships between structure and function in this protein. Over a five-year period, the Candidate is funded to 1) generate site-directed mutations in bacterial reaction centers, 2) characterize photochemical effects of structural perturbations, 3) characterize the nature of structural perturbations using indirect spectroscopic and biochemical techniques and 4) interpret these observations using physical theories.

该提案要求为C颁发研究职业发展奖。 Schenck。 候选人的长期研究目标是 了解膜的结构和功能之间的关系 蛋白质。 其他长期职业目标包括参与 为研究生建立刺激的培训环境 科罗拉多州生物化学和生物物理学博士后研究 大学。 解决问题的多学科方法将是 鼓励。 朝着这些目标迈出的第一步是发展 有力的独立研究计划。 RCDA将允许候选人 将完全关注与研究和研究相关的活动集中 五年。 具体而言，RCDA将：1）减少非 - 研究相关的教学和行政活动； 2）允许 候选人在两个研究领域发展专业知识，与 他自己的研究，但需要进一步的培训； 3）受益 通过鼓励与AT协作研究互动的部门 在越来越多的研究方面，至少有另外两名教职员工 部门。 细菌反应中心是一个有趣的测试系统 生物电子转移和蛋白质动态行为的理论。 研究计划描述了候选人的短期研究目标 评估结构与功能之间的关系 蛋白质。 在五年的时间内，候选人被资助为1） 细菌反应中心中的位置突变，2）表征 结构扰动的光化学效应，3）表征 使用间接光谱和结构扰动的性质 生化技术和4）使用物理来解释这些观察 理论。