SOLID STATE STRUCTURAL NMR OF LH1 PROTEIN

LH1 蛋白的固态结构核磁共振

• 批准号: 2805711
• 负责人: CRAIG C SCHENCK
• 金额: $ 3.7万
• 依托单位: COLUMBIA UNIV NEW YORK MORNINGSIDE
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-12-16 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/2805711
• 关键词: chemical models; chlorophyll; detergents; electron spin resonance spectroscopy; micelles; nuclear magnetic resonance spectroscopy; protein structure; solid state

The last decade has witnessed a remarkable growth of knowledge in structural biology. A class of protein particularly important for biological energy transduction, membrane proteins, has begun to yield structural information by high-resolution methods. These developments notwithstanding, our current knowledge-base of membrane-protein structure is very limited; 103 globular protein structures have been reported, but only 101 membrane protein structures are known. Furthermore, consider that approximately 30 percent of structural genes in a free-living microbe are believed to encode membrane proteins, and one concludes that membrane protein structure is an important area of structural biology that has been overlooked. Likewise, detailed examination of the molecular forces that fold and stabilize membrane proteins has lagged behind globular proteins. The Applicant proposes to undertake novel approaches to these problems with solid state NMR spectroscopy. The high resolution structure and dynamics of an oligomeric membrane protein, the LH1 light-harvesting complex from photosynthetic bacteria, will be studied.

最近十年见证了知识的显着增长 结构生物学。 一类蛋白质对于 生物能转导，膜蛋白已开始产生 高分辨率方法的结构信息。这些发展 尽管如此，我们目前的膜蛋白知识基础 结构非常有限； 103球蛋白结构已经 报道，但只有101种膜蛋白结构已知。 此外，考虑大约30％的结构基因 在自由生存的微生物中，据信编码膜蛋白，并且 一个人得出结论，膜蛋白结构是 被忽视的结构生物学。 同样，详细 检查折叠和稳定膜的分子力 蛋白质落后于球状蛋白。 申请人提出 为解决固态NMR的这些问题采取新颖的方法 光谱法。 高分辨率结构和动态 低聚的膜蛋白，LH1轻填充复合物 将研究光合细菌。