AGING AND CENTRAL AUDITORY SYSTEM MORPHOLOGY

衰老与中枢听觉系统形态

基本信息

批准号：
2413308
负责人：
JAMES F WILLOTT
金额：
$ 10万
依托单位：
NORTHERN ILLINOIS UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
1988
资助国家：
美国
起止时间：
1988-05-01 至 1998-04-30
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/2413308
关键词：
aging animal age group auditory nuclei auditory pathways brain stem cell type dendrites genotype glia histology inferior colliculus laboratory mouse neuroanatomy presbycusis psychoacoustics sound frequency

项目摘要

Central anatomical correlates of aging and age-related hearing loss (presbycusis) will be studied in two mouse models: the C57BL/6J, which demonstrates progressive sensorineural hearing loss (SNHL) with onset during young adulthood to middle age; and the CBA/J, which maintains good auditory sensitivity until late in life. Several age groups will be studied, ranging from young (2-months old) to very old (over 30-months). Two groups of CBA mice with induced chronic SNHL (similar to that occurring in C57 mice) will also be employed. Quantitative morphometric evaluations will be made in subdivisions of the cochlear nucleus (CN) and inferior colliculus (IC) using Nissl-stained, fiber-stained, and Golgi- impregnated tissue. A variety of cellular features will be assessed including cell number (neurons and glia), neuronal soma size and shape, dendritic dimensions and orientation, presence of spines, signs of degeneration, etc. Data will be statistically analyzed as a function of age, brainstem subnucleus, and when appropriate, cell type or location with regard to tonotopic organization. Peripheral input to the brainstem will be evaluated using quantitative counts of spiral ganglion cells. The final product will be a comprehensive, detailed anatomical description of two major auditory brainstem regions in two mouse models of presbycusis. In addition, by comparisons among data from the various subject groups the contributions of aging per se, the occurrence and chronicity of SNHL, and genotype will be elucidated.

衰老和年龄相关性听力损失的中心解剖学相关性（老年性耳聋）将在两种小鼠模型中进行研究：C57BL/6J、这表明进行性感音神经性听力损失（SNHL）在青年期至中年期间发病；和 CBA/J，直到晚年仍保持良好的听觉敏感性。将研究几个年龄组，从年轻的（2个月大的老）到非常老（超过 30 个月）。两组 CBA 小鼠诱导的慢性 SNHL（类似于 C57 小鼠中发生的情况）将也能就业。定量形态测量评估将由耳蜗核 (CN) 和下核细分而成使用尼氏染色、纤维染色和高尔基染色的丘 (IC) 浸渍的组织。各种蜂窝功能将评估包括细胞数量（神经元和神经胶质细胞）、神经元胞体尺寸和形状、枝晶尺寸和方向、存在刺、退化迹象等。数据将进行统计分析为年龄、脑干亚核以及何时与音位相关的适当的细胞类型或位置组织。将评估脑干的外周输入使用螺旋神经节细胞的定量计数。最终产品将是一个全面、详细的解剖学两只小鼠的两个主要听觉脑干区域的描述老年性耳聋模型。另外，通过数据对比各个主题组老龄化本身的贡献 SNHL 的发生和慢性性以及基因型将得到阐明。