WOUND HEALING ACCELERATION USING NATURAL PRODUCTS

使用天然产品加速伤口愈合

• 批准号: 2810705
• 负责人: GERALD B HAMMOND
• 金额: $ 10.75万
• 依托单位: UNIVERSITY OF MASSACHUSETTS DARTMOUTH
• 依托单位国家: 美国
• 财政年份: 1999
• 资助国家: 美国
• 起止时间: 1999-05-01 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2810705
• 关键词: DNA topoisomerases; Peru; antineoplastics; bioassay; cell migration; cell proliferation; chemical structure function; drug discovery /isolation; enzyme inhibitors; medicinal plants; plant extracts; wound healing

This renewal proposal describes an interdisciplinary, systematic and targeted study of Peruvian plants with cicatrizant (wound-healing) activity. Although cicatrizant plants constitute the main focus of this proposal, extracts will also be screened for potential antineoplasic activity. The diverse topography, semitropical climate and unique cultural heritage of Peru contribute to making this country a desirable place for bioprospecting. Cicatrizant agents could help patients with illness such as diabetes, gastric ulcers or varicose ulcers, whose wounds do not scar easily. Notwithstanding these medical benefits, the scientific study of wound-healing plants may help uncover whether a connection can be established between injuries, healing and the treatment of proliferative disorders. Recent work has uncovered evidence that strengthens a correlation between injury and carcinogenesis. The study of cicatrizant plants and the mechanisms by which their active principle(s) operate, will reveal natural agents capable of promoting tissue regeneration. This investigation will shed light on externally promoted healing mechanisms triggered by acute and, perhaps chronic, injury. A five-step approach to wound-healing natural products has been designed: 1. Characterization of the active principle from Anredera diffusa and bioassay-guided fractionation of Jatropa curcas latex. The former was isolated during the previous grant period. 2. Structure-activity relationship studies on taspine and (+)-anymol, the two cicatrizant principles found in Croton lechleri and Peperomia galioides, respectively. The latter was isolated and identified during the previous grant period. 3. Cell migration and cell proliferation studies to probe mechanism of action for cicatrizant principles found in Peperomia galioides, Anredera diffusa and Jatropa curcas. 4. Characterization of the active principle from an extract who failed to show in vivo wound-healing activity during the previous grant period but tested positive in our anticancer screening assay. This corresponds to a potential topoisomerase II inhibitor from Campsiandra angustifolia. 5. Scientific evaluation of crude extracts from newly identified Peruvian plants with cicatrizant and anticancer activity. The long-term objective of this research is to strengthen the international and multidisciplinary collaboration between scientists from Peru and the United States, with the aim of linking conservation of biological diversity with sustained economic management of medicinal plants needed for the discovery and development of pharmaceuticals to improve human health.

该更新提案描述了秘鲁植物具有cicatrizant（伤口 - 修复）活性的跨学科，系统和有针对性的研究。 虽然美卫生植物构成 该提案的主要重点，还将筛选提取物以进行潜在的抗肿瘤活性。 秘鲁的各种地形，半趋化气候和独特的文化遗产 为使这个国家成为生物培训的理想场所。 甲状腺素剂可以帮助患有疾病的患者，例如糖尿病，胃溃疡或静脉曲张溃疡，其伤口不容易疤痕。 尽管有这些医疗益处， 伤口治疗植物的科学研究可能有助于发现是否可以在伤害，愈合和增殖性疾病治疗之间建立联系。 最近的工作发现了加强损伤与癌变之间相关性的证据。 核植物的研究及其主动原理运行的机制将 揭示能够促进组织再生的天然药物。 这项调查将揭示出急性和慢性伤害触发的外部促进的愈合机制。五步方法是伤口愈合天然产品 设计：1。从Anredera diffusa和Jatropa Curcas乳胶的生物测定指导分馏的活性原理的表征。 前者是在上一个赠款中孤立的 时期。 2。对taspine和（+）的结构活性关系研究，分别在Croton Lechleri和Galioides中分别发现的两种环化原理。 后者是 在上一个赠款期间被隔离和确定。 3。细胞迁移和细胞增殖研究，以探测胸膜细胞中发现的作用机理 Galioides，Anredera diffusa和Jatropa Curcas。 4。从未能在上一个未能显示体内伤口愈合活性的提取物中表征活性原理的表征 赠款期，但在我们的抗癌筛查测定中测试呈阳性。 这对应于Campsiandra angustifolia的潜在拓扑异构酶II抑制剂。 5。对新鉴定出的具有果酱和抗癌活性的秘鲁植物的粗提物的科学评估。 这项研究的长期目标是加强秘鲁和美国科学家之间的国际和多学科合作，目的 将生物多样性的保护与发现和开发药物以改善人类健康所需的药用植物的持续经济管理联系起来。