RECOMBINANT ANTIBODIES ENGINEERED FOR DNA ADDUCTS

针对 DNA 加合物设计的重组抗体

• 批准号: 2909990
• 负责人: SRINIVASAN CHANDRASEGARAN
• 金额: $ 22.49万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2909990
• 关键词: Escherichia coli; adduct; chimeric proteins; endonuclease; enzyme structure; hybrid antibody; hybridomas; protein engineering; recombinant proteins; synthetic enzyme; transfection

DESCRIPTION: (Adapted from the Investigator's Abstract) The investigators have obtained a one year starter grant and showed good progress toward Aim I. Aim I is to take antibody genes, genes that code for antibody recognition sites specific for adducts, from hybridomas. Then functional recombinant Ab fragments, ScFv fragments, are assembled as fusion proteins on the surface of bacteriophage M13 and ScFv fragment-gIIIp fusion proteins subsequently are overproduced in E. coli. The second aim is to alter the specificity and affinity of the proteins to adducts by genetic manipulation. The third aim is to construct chimeric endonucleases consisting of an ScFv fragment as the recognition domain and the FokI cleavage domain.

描述：（改编自调查员摘要）调查员 已获得一年的启动补助金，并在目标方面取得了良好的进展 I. 目标I是获取抗体基因，编码抗体识别的基因 来自杂交瘤的加合物的特异性位点。 那么功能性重组抗体 片段，ScFv片段，在表面组装为融合蛋白 随后噬菌体 M13 和 ScFv 片段-gIIIp 融合蛋白 在大肠杆菌中过量产生。 第二个目标是改变特异性和 通过基因操作使蛋白质与加合物的亲和力。 第三个目标 是构建由ScFv片段组成的嵌合核酸内切酶作为 识别域和 FokI 切割域。