NEONATAL PROXIMAL TUBULAR ACIDIFICATION

新生儿近端肾小管酸化

• 批准号: 2905395
• 负责人: MICHEL GERARD BAUM
• 金额: $ 19.41万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-02-01 至 2000-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2905395
• 关键词: SDS polyacrylamide gel electrophoresis; angiotensin II; biological signal transduction; catecholamines; dopamine; embryo /fetus chemotherapy; embryo /fetus drug adverse effect; glucocorticoids; ion transport; kidney; laboratory rabbit; nephrectomy; newborn animals; parathyroid hormones; phosphates; polymerase chain reaction; postnatal growth disorder; renal tubular transport; renal tubule acidosis; respiratory acidosis; sodium chloride; statistics /biometry; western blottings

Glucocorticoids are often administered to pregnant women to accelerate lung maturation and prevent respiratory distress syndrome. The effect of glucocorticoids on the developing kidney is unknown. The first aim of this proposal is to examine whether prenatal glucocorticoids affect nephrogenesis in fetal rabbits. Glucocorticoids have also been shown to play a role in the maturation of several organs. We plan to examine if the postnatal increase in glucocorticoid levels are responsible for the maturational increase in proximal tubule acidification and decrease in proximal tubule phosphate transport. Premature neonates have marked urinary losses of NaCl and are in negative salt balance causing dehydration and hyponatremia. The majority of NaCl transport in adult animals is by the proximal tubule. However, the mechanism of proximal tubule NaCl transport by the premature and term neonate is unknown. The second aim of this proposal is to use in vitro microperfusion to examine the mechanism and regulation of NaCl transport and factors which may play a role in maturation of NaCl transport with renal development. The final aim of this proposal focuses on the signal transduction system in neonatal proximal tubule. Several hormones which regulate adult proximal tubule transport have a blunted or no response in the neonatal proximal tubule. The reason, for this block in signal transduction is unknown. We will examine why the response to PTH and dopamine are attenuated in the neonate and if glucocorticoids affect the maturation of signal transduction.

孕妇经常服用糖皮质激素以加速妊娠 促进肺部成熟并预防呼吸窘迫综合征。的效果 糖皮质激素对发育中肾脏的影响尚不清楚。本次活动的第一个目的 建议检查产前糖皮质激素是否会影响 胎兔肾发生。糖皮质激素也被证明可以 在多个器官的成熟中发挥作用。我们计划检查是否 产后糖皮质激素水平的增加是造成 近曲小管酸化的成熟增加和 近曲小管磷酸盐转运。 早产儿尿液中氯化钠明显流失，呈阴性 盐平衡导致脱水和低钠血症。大部分是氯化钠 成年动物的运输是通过近端小管进行的。然而， 早产儿和足月儿近曲小管 NaCl 转运机制 新生儿未知。该提案的第二个目标是在体外使用 微灌注研究 NaCl 转运的机制和调节 以及哪些因素 可能在肾脏发育过程中 NaCl 运输的成熟中发挥作用。 该提案的最终目标集中在信号转导系统上 在新生儿近曲小管中。调节成人的几种激素 近曲小管运输在新生儿中反应迟钝或无反应 近端小管。信号转导中的这种阻碍的原因是 未知。我们将研究为什么对 PTH 和多巴胺的反应是 在新生儿中减弱，并且如果糖皮质激素影响成熟 信号转导。