MECHANISMS OF APOPTOSIS IN BLOOD CELLS

血细胞凋亡机制

• 批准号: 2871453
• 负责人: Roberta A. Gottlieb
• 金额: $ 27.33万
• 依托单位: SCRIPPS RESEARCH INSTITUTE, THE
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-02-10 至 2001-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2871453
• 关键词: DNA damage; acidity /alkalinity; antiport; apoptosis; blood cells; chloride channels; clinical research; clone cells; complementary DNA; cystic fibrosis; endonuclease; enzyme activity; gene deletion mutation; genetic library; genetic promoter element; human subject; hydrogen; membrane transport proteins; mitochondria; molecular cloning; neutrophil; protein purification; sodium

DESCRIPTION (Adapted From the Applicant's Abstract): Apoptosis is the name of the stereotyped program by which cells commit suicide. It is characterized by destruction of chromatin and degradation of the DNA, usually to nucleosomal fragments, accompanied by cell shrinkage and loss of cell substance through blabbing. Apoptosis is of wide physiological and pathological significance, important in embryogenesis, control of tissue growth and the regulation of the immune system, as well as in the elimination of cells that have sustained serious damage, particularly to their genomes. The Investigators have shown that in several lines of cells exposed to a variety of apoptosis-inducing agents, apoptosis with its accompanying genome destruction is preceded by intracellular acidification, and that the genome is preserved if this acidification is prevented. They propose to investigate the mechanism of acidification in cells undergoing apoptosis, and to study a ubiquitous acid endonuclease that may be responsible for the degradation of DNA during the apoptosis program.

描述（改编自申请人的摘要）：细胞凋亡是 细胞自杀的定型程序的名称。 这是 其特点是染色质破坏和 DNA 降解， 通常为核小体碎片，伴有细胞收缩和损失 通过吹出细胞物质。 细胞凋亡具有广泛的生理学意义 和病理意义，在胚胎发生、控制中很重要 组织生长和免疫系统的调节，以及 消除遭受严重损伤的细胞，特别是 他们的基因组。 研究人员已经表明，在几行中 细胞暴露于多种凋亡诱导剂后，细胞凋亡 其伴随的基因组破坏发生在细胞内 酸化，并且如果这种酸化是基因组被保存 阻止了。 他们建议研究酸化机制 在经历凋亡的细胞中，并研究普遍存在的酸 核酸内切酶可能是 DNA 降解的原因 细胞凋亡程序。