DEVELOPMENT OF AN ELECTRONIC TONGUE

电子舌头的发展

• 批准号: 2910393
• 负责人: Eric V. Anslyn
• 金额: $ 24.75万
• 依托单位: UNIVERSITY OF TEXAS AT AUSTIN
• 依托单位国家: 美国
• 财政年份: 1998
• 资助国家: 美国
• 起止时间: 1998-05-01 至 2001-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/2910393
• 关键词: analytical chemistry; biomedical equipment development; charge coupled device camera; colorimetry; fluorescence spectrometry; peptide library; taste

DESCRIPTION: This grant application is focused upon fabricating arrays of sensor elements that will create specific patterns when exposed to mixtures of analytes. These patterns will allow for the simultaneous identification of the individual analytes in the mixture. As a proof of concept, we will use the sensor arrays to target common analytes in blood and urine samples. The inspiration for our sensing strategy derives from the mammalian sense of taste The sense of taste in mammals derives from both specific and nonspecific molecular recognition events at hundreds to thousands of receptor sites. Only four different types of signals are created: sweet, sour, salty, and bitter. Yet, the different combinations resulting from the absence and presence as wel as the intensity of these four signals is enough to create patterns that are recognized by the brain to be specific for different foods. It is the ability to use molecular recognition in a nonspecific manner to create a fingerprint for each taste that allows mammals to be very sensitive and selective with respect to food differentiation. The "electronic tongue" will be composed of polystyrene/polyethylene glycol resin beads that have been derivatized with libraries of nucleotides, peptides, thioureas, and guanidiniums. The libraries will also possess monomers that have been shown to induce secondary structures Further, fluorescent monomers that are exquisitely sensitive to local microenvironment will be incorporated into the libraries. The libraries will b screened for interactions with specific guests, and any members of the library showing specific or nonspecific recognition events will be incorporated into a micromachined platform. This platform will be comprised of wells for depositio of the beads, flow channels, UV/vis filters, and a charged-coupled device (CCD). Irradiation of the entire library with excitation light will create colorimetric/fluorescent signals resulting in patterns specific for the analyt solutions. These patterns will be stored in a computer for future reference, and for identification and quantitation of analytes in unknown samples.

描述：此赠款应用的重点是制造 传感器元素将在暴露于混合物时会产生特定模式 分析物。 这些模式将允许同时识别 混合物中的单个分析物。 作为概念的证明，我们将 使用传感器阵列靶向血液和尿液样品中的常见分析物。 我们的感应策略的灵感来自于哺乳动物的意义 品尝哺乳动物的味道感来自特定和 非特异性分子识别事件在数百到数千个 受体位点。 仅创建了四种不同类型的信号：甜， 酸，咸和苦。 然而，由 缺乏和存在像这四个信号的强度一样的温度就足够了 创建大脑认可的模式特定于 不同的食物。 这是在A中使用分子识别的能力 非特异性的方式，为每种味道创建指纹 哺乳动物对食物非常敏感和有选择性 分化。 “电子舌头”将由 聚苯乙烯/聚乙烯乙二醇树脂珠已被衍生 核苷酸，肽，thioureas和鸟肾的文库。 这 图书馆也将拥有已显示诱导的单体 进一步的二级结构，精美的荧光单体 对局部微环境敏感的库将纳入库中。 图书馆将B筛选与特定客人的互动，以及任何 图书馆的成员显示特定或非特异性识别事件 将合并到微机械平台中。 这个平台将是 由用于珠子，流通通道，紫外线/VIS的井组成 过滤器和带电耦合的设备（CCD）。 整个辐照 带有激发灯的库将产生比色/荧光信号 导致针对分析解决方案的模式。 这些模式 将存储在计算机中以备将来参考和识别 和未知样品中分析物的定量。