MULTI-ANALYTE MICRO-DEVICES FOR BIOMEDICAL APPLICATIONS

用于生物医学应用的多分析物微型设备

• 批准号: 6920620
• 负责人: Joseph Andrade
• 金额: $ 31.95万
• 依托单位: UNIVERSITY OF UTAH
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-08-01 至 2007-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6920620
• 关键词: analytical chemistry; bioengineering /biomedical engineering; bioluminescence; biomedical equipment development; biosensor device; blood; charge coupled device camera; chemical structure; enzymes; fluid; galactose; galactosemias; hemodialysis; lactates; light emission; lyophilization; monitoring device; reagent /indicator; semiconduction; solutions; urine

DESCRIPTION (provided by applicant): The investigators propose to develop microdevices for the specific chemical analysis of multiple metabolites in small sample volumes of biological fluids. The specificity and sensitivity is provided by specific reactions that couple analytes to bioluminescent-based enzyme reactions and produce light proportional to the analyte concentration. Bioluminescent analytical assays, in a miniaturized and stable format, can measure sub-microMolar concentrations in microliter sample volumes. The goal of the first phase (R21) is to engineer microfluidic structures, develop enzyme packaging and stabilization techniques, and optimize optical detection systems in order to measure two model analyte solutions (galactose and lactate) using bioluminescent reactions. The goal of the second phase (R33) is to implement other bioluminescent assays in the microfluidic detection system, develop specific diagnostic panels, utilize practical biofluid samples and enhance analytical accuracy and precision. The proposed Micro-Analytical System (microAS) will be convenient to operate in point-of-care (POC) and home environments. It will likely evolve to measure up to 100 different metabolites in the submicroMolar to milliMolar range from one 1-100 microL biofluid sample, and include customized comprehensive diagnostic panels for basic research, clinical research, and for personal disease and health management. These systems would provide rapid results, facilitate patient empowerment, and reduce health care costs.

描述（由申请人提供）：研究人员提议开发微型发行版，以在少量的生物流体样本体积中对多种代谢产物进行特定化学分析。 特定反应提供了特异性和敏感性，这些反应将分析对基于生物发光的酶反应进行分析，并产生与分析物浓度成正比的光。 以微型和稳定格式的生物发光分析测定可以测量微氧样品体积中的微摩尔摩尔浓度。 第一阶段（R21）的目的是设计微流体结构，开发酶包装和稳定技术，并优化光学检测系统，以便使用生物发光反应测量两种模型分析物溶液（半乳糖和乳酸）。 第二阶段（R33）的目的是在微流体检测系统中实施其他生物发光测定，开发特定的诊断面板，利用实用的生物流体样本并提高分析精度和精度。 拟议的微分析系统（Microas）将方便在护理（POC）和家庭环境中运行。 它可能会进化，在亚摩尔尔摩尔极摩尔到毫米范围内测量多达100种不同的代谢产物，从一个1-100 microl生物流体样本，并包括用于基础研究，临床研究以及个人疾病和健康管理的定制综合诊断板。 这些系统将提供快速的结果，促进患者赋权并降低医疗保健成本。